Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFYH6_RS08070 Genome accession   NZ_CP170704
Coordinates   1690092..1691147 (+) Length   351 a.a.
NCBI ID   WP_416179506.1    Uniprot ID   -
Organism   Clostridium sp. HCS.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1685092..1696147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFYH6_RS08050 (ACFYH6_08050) - 1685569..1686804 (+) 1236 WP_416179497.1 peptidase U32 family protein -
  ACFYH6_RS08055 (ACFYH6_08055) udk 1686826..1687443 (+) 618 WP_316342771.1 uridine kinase -
  ACFYH6_RS08060 (ACFYH6_08060) - 1687505..1689121 (+) 1617 WP_416179496.1 penicillin-binding transpeptidase domain-containing protein -
  ACFYH6_RS08065 (ACFYH6_08065) sigK 1689287..1689982 (+) 696 WP_291708114.1 RNA polymerase sporulation sigma factor SigK -
  ACFYH6_RS08070 (ACFYH6_08070) pilT 1690092..1691147 (+) 1056 WP_416179506.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFYH6_RS08075 (ACFYH6_08075) ftsZ 1691646..1692767 (+) 1122 WP_316342773.1 cell division protein FtsZ -
  ACFYH6_RS08080 (ACFYH6_08080) - 1692992..1693795 (+) 804 WP_416179495.1 sigma-E processing peptidase SpoIIGA -
  ACFYH6_RS08085 (ACFYH6_08085) sigE 1693792..1694499 (+) 708 WP_316342775.1 RNA polymerase sporulation sigma factor SigE -
  ACFYH6_RS08090 (ACFYH6_08090) sigG 1694580..1695353 (+) 774 WP_291631424.1 RNA polymerase sporulation sigma factor SigG -
  ACFYH6_RS08095 (ACFYH6_08095) - 1695420..1695731 (+) 312 WP_316342776.1 YlmC/YmxH family sporulation protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39024.18 Da        Isoelectric Point: 6.9837

>NTDB_id=1058604 ACFYH6_RS08070 WP_416179506.1 1690092..1691147(+) (pilT) [Clostridium sp. HCS.1]
MYVLNELLKETITRGASDLHLTVGLPPTIRVNGKLLKIGEEKLTPIALRNFAEEILEDKYKEYDDYGEMDTSYSVAGTGR
FRVNIFKQRNSDAIAIRVIALKIPTLDELKHPKIIKNIIEKQRGLVLVTGPTGSGKSTTLAAMINEINNNREAHIITLED
PIEYLHKHNKCIINQREIGKDSKSYQNALKAILREDPDVILVGEMRDLETISIAITAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQIRIQLAAVLQGVISQQLVPLVNNDGRVAALEIMTTTPGIQNLIREGKTHQIESAIQTGGKYGMKTMDMA
LADLVKKGSITEETALTYSVDNETIKRIMML

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1058604 ACFYH6_RS08070 WP_416179506.1 1690092..1691147(+) (pilT) [Clostridium sp. HCS.1]
ATTTATGTGCTAAATGAACTATTGAAAGAGACAATAACTAGAGGAGCCTCAGATTTACATTTAACTGTGGGATTGCCACC
AACAATTAGAGTTAATGGAAAGCTATTAAAGATTGGTGAAGAAAAATTAACTCCTATAGCATTAAGAAACTTTGCAGAAG
AAATATTAGAAGATAAATATAAAGAGTACGATGATTATGGTGAAATGGATACATCTTATTCAGTAGCAGGTACTGGAAGA
TTTAGGGTGAATATATTTAAACAAAGAAATAGTGATGCTATAGCTATTAGAGTAATAGCATTAAAAATACCCACATTAGA
TGAATTAAAACATCCAAAGATAATAAAGAACATAATTGAAAAACAAAGAGGATTAGTACTAGTAACTGGACCAACTGGAA
GTGGGAAAAGCACTACCTTAGCAGCTATGATTAATGAAATAAATAATAATAGGGAAGCTCACATCATTACCTTAGAAGAT
CCAATAGAATACTTGCATAAGCATAATAAATGCATTATTAATCAAAGAGAAATAGGAAAAGACAGTAAAAGCTATCAAAA
TGCTTTAAAAGCAATTTTAAGAGAAGACCCAGATGTAATACTTGTAGGTGAGATGAGGGATTTAGAGACAATTTCAATTG
CAATTACTGCAGCAGAAACTGGACATCTAGTGTTCTCCACACTACATACAATTGGTGCTGCTAAAACTATAGATAGAATA
ATTGATGTTTTTCCACCTCATCAACAGCAACAAATTAGAATCCAATTAGCTGCTGTATTACAAGGGGTTATTTCACAGCA
ATTAGTACCCTTAGTAAATAATGATGGAAGAGTAGCTGCCTTAGAAATAATGACAACAACTCCTGGAATTCAGAATTTAA
TTAGAGAAGGAAAAACTCACCAAATTGAATCTGCAATTCAAACTGGTGGAAAATATGGAATGAAGACAATGGATATGGCC
TTAGCTGATTTAGTTAAGAAAGGTAGCATTACTGAAGAAACAGCGTTAACATATTCAGTAGATAATGAAACAATAAAAAG
AATAATGATGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

51.312

97.721

0.501
  pilT Acinetobacter baumannii D1279779

51.312

97.721

0.501
  pilT Acinetobacter baumannii strain A118

51.312

97.721

0.501
  pilT Acinetobacter baylyi ADP1

52.553

94.872

0.499
  pilT Pseudomonas aeruginosa PAK

52.553

94.872

0.499
  pilT Legionella pneumophila strain Lp02

51.497

95.157

0.49
  pilT Legionella pneumophila strain ERS1305867

51.497

95.157

0.49
  pilT Pseudomonas stutzeri DSM 10701

51.051

94.872

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.84

92.877

0.481
  pilT Vibrio cholerae strain A1552

51.84

92.877

0.481
  pilT Neisseria gonorrhoeae MS11

49.699

94.587

0.47
  pilT Neisseria meningitidis 8013

49.699

94.587

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.821

98.575

0.462
  pilU Vibrio cholerae strain A1552

42.241

99.145

0.419
  pilU Pseudomonas stutzeri DSM 10701

41.667

99.145

0.413
  pilU Acinetobacter baylyi ADP1

40.237

96.296

0.387

Multiple sequence alignment