Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFK9X_RS19975 Genome accession   NZ_CP170629
Coordinates   4085441..4086421 (-) Length   326 a.a.
NCBI ID   WP_045901825.1    Uniprot ID   A0A5T3MD10
Organism   Salmonella sp. WC3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4080441..4091421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFK9X_RS19945 (ACFK9X_19950) endA 4080893..4081600 (+) 708 WP_151117489.1 deoxyribonuclease I -
  ACFK9X_RS19950 (ACFK9X_19955) rsmE 4081677..4082408 (+) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACFK9X_RS19955 (ACFK9X_19960) gshB 4082428..4083375 (+) 948 WP_000593248.1 glutathione synthase -
  ACFK9X_RS19960 (ACFK9X_19965) - 4083591..4084154 (+) 564 WP_001053167.1 YqgE/AlgH family protein -
  ACFK9X_RS19965 (ACFK9X_19970) ruvX 4084154..4084570 (+) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  ACFK9X_RS19970 (ACFK9X_19975) - 4084617..4085309 (-) 693 WP_151117492.1 IclR family transcriptional regulator -
  ACFK9X_RS19975 (ACFK9X_19980) pilT 4085441..4086421 (-) 981 WP_045901825.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFK9X_RS19980 (ACFK9X_19985) - 4086439..4087143 (+) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFK9X_RS19985 (ACFK9X_19990) - 4087162..4087728 (+) 567 WP_001094848.1 YggT family protein -
  ACFK9X_RS19990 (ACFK9X_19995) yggU 4087725..4088015 (+) 291 WP_001277205.1 DUF167 family protein YggU -
  ACFK9X_RS19995 (ACFK9X_20000) - 4088023..4088616 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  ACFK9X_RS20000 (ACFK9X_20005) hemW 4088609..4089745 (+) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  ACFK9X_RS20005 (ACFK9X_20010) - 4089837..4090844 (-) 1008 WP_151117494.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36137.38 Da        Isoelectric Point: 8.0973

>NTDB_id=1058237 ACFK9X_RS19975 WP_045901825.1 4085441..4086421(-) (pilT) [Salmonella sp. WC3]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1058237 ACFK9X_RS19975 WP_045901825.1 4085441..4086421(-) (pilT) [Salmonella sp. WC3]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCAGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5T3MD10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362

Multiple sequence alignment