Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJIW_RS16695 Genome accession   NZ_CP170554
Coordinates   4019078..4020178 (+) Length   366 a.a.
NCBI ID   WP_386066171.1    Uniprot ID   -
Organism   Tahibacter sp. UC22_41     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4014078..4025178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIW_RS16675 (ACFJIW_16675) - 4014268..4015395 (-) 1128 WP_386066167.1 cupin domain-containing protein -
  ACFJIW_RS16680 (ACFJIW_16680) kduD 4015437..4016195 (-) 759 WP_386066168.1 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD -
  ACFJIW_RS16685 (ACFJIW_16685) kduI 4016203..4017051 (-) 849 WP_386066169.1 5-dehydro-4-deoxy-D-glucuronate isomerase -
  ACFJIW_RS16690 (ACFJIW_16690) - 4017490..4019073 (+) 1584 WP_386066170.1 GspE/PulE family protein -
  ACFJIW_RS16695 (ACFJIW_16695) pilU 4019078..4020178 (+) 1101 WP_386066171.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIW_RS16700 (ACFJIW_16700) - 4020213..4021271 (-) 1059 WP_386066172.1 LacI family DNA-binding transcriptional regulator -
  ACFJIW_RS16705 (ACFJIW_16705) - 4021417..4022697 (-) 1281 WP_386066173.1 TRAP transporter large permease -
  ACFJIW_RS16710 (ACFJIW_16710) - 4022697..4023029 (-) 333 WP_386066174.1 TRAP transporter small permease subunit -
  ACFJIW_RS16715 (ACFJIW_16715) - 4023282..4024274 (-) 993 WP_386066175.1 TRAP transporter substrate-binding protein -
  ACFJIW_RS16720 (ACFJIW_16720) - 4024271..4025095 (-) 825 WP_386066176.1 2-keto-4-pentenoate hydratase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40826.92 Da        Isoelectric Point: 6.0929

>NTDB_id=1057967 ACFJIW_RS16695 WP_386066171.1 4019078..4020178(+) (pilU) [Tahibacter sp. UC22_41]
MPELTSYMQLMARKKASDCFLSVGAPPSIKIEGETTHLGETALTAEDVRGMAYSIMSEKQQKEFEATLEMNLAVALADGS
RFRINVYRQRDTVAIAVRYISSDIPSLDQLSLPPKLKDLIMLPRGLVLVVGSTGSGKSTTLASMIDHRNHHRTGHILTIE
EPIEFIHRHDKSVVDQREIGLDTLSYENALKNAMREAPDVILIGEIRDRETMKHAIAYAETGHLCLSTLHANNANQTLDR
IINFFPDSARHQLLIDLSLNLQAVVAMRLVKGLDGRRTPAVELLLRTAYVADLIEKGEIDQLKEAMKQGVNIGMQTFDEA
LYRLYALGRISLAEALDHADSRTDLSLRVRLSNPVEAMSDLRMEDI

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1057967 ACFJIW_RS16695 WP_386066171.1 4019078..4020178(+) (pilU) [Tahibacter sp. UC22_41]
ATGCCCGAACTGACGAGCTACATGCAGCTGATGGCGCGCAAGAAGGCTTCCGACTGCTTTCTCAGCGTCGGCGCGCCGCC
GTCGATCAAGATCGAGGGCGAGACCACGCACCTGGGCGAGACCGCGCTGACCGCCGAGGACGTGCGCGGCATGGCGTACT
CGATCATGAGCGAAAAGCAGCAGAAGGAATTCGAGGCGACCCTGGAGATGAATCTGGCCGTCGCGCTGGCCGACGGCAGC
CGCTTCCGCATCAACGTCTACCGGCAGCGCGACACCGTCGCGATCGCCGTGCGCTACATCAGCAGCGACATCCCGTCGCT
GGACCAGCTGTCGCTGCCGCCCAAGCTCAAGGATCTGATCATGCTGCCGCGCGGGCTCGTGCTCGTCGTCGGCAGCACCG
GCTCGGGCAAGTCGACGACCCTGGCGTCGATGATCGACCACCGCAACCATCACCGTACCGGCCATATCCTGACCATCGAG
GAGCCGATCGAATTCATCCACCGCCACGACAAGTCGGTCGTCGACCAGCGCGAGATCGGCCTGGACACGCTGTCCTACGA
GAACGCGCTCAAGAACGCCATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGAAGC
ACGCGATCGCCTATGCGGAAACCGGTCATCTGTGCCTGTCGACCCTGCACGCCAACAACGCCAACCAGACCTTGGACCGC
ATCATCAACTTCTTTCCGGACTCGGCGCGCCACCAGCTGCTGATCGACCTGTCCCTGAACCTGCAGGCCGTCGTCGCGAT
GCGCCTGGTCAAGGGCCTGGACGGCCGGCGCACGCCGGCCGTGGAACTGCTGCTGCGTACCGCCTATGTGGCCGACCTCA
TCGAGAAGGGCGAGATCGACCAGCTCAAGGAGGCCATGAAACAAGGGGTCAACATCGGCATGCAGACCTTCGACGAGGCG
CTGTACCGTCTCTACGCGCTGGGCAGGATCAGCCTGGCCGAAGCGCTGGATCACGCCGACTCGCGCACCGACCTGTCGCT
GCGCGTACGCCTGTCCAATCCGGTCGAAGCCATGAGCGACCTGCGCATGGAGGACATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

51.558

96.448

0.497
  pilU Pseudomonas stutzeri DSM 10701

50.281

97.268

0.489
  pilU Vibrio cholerae strain A1552

50.43

95.355

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.539

97.268

0.423
  pilT Vibrio cholerae strain A1552

40.173

94.536

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.173

94.536

0.38
  pilT Neisseria gonorrhoeae MS11

39.884

94.536

0.377
  pilT Neisseria meningitidis 8013

39.884

94.536

0.377
  pilT Legionella pneumophila strain ERS1305867

42.547

87.978

0.374
  pilT Legionella pneumophila strain Lp02

42.547

87.978

0.374
  pilT Acinetobacter baylyi ADP1

40.841

90.984

0.372
  pilT Pseudomonas aeruginosa PAK

40.059

92.077

0.369
  pilT Pseudomonas stutzeri DSM 10701

39.763

92.077

0.366
  pilT Acinetobacter baumannii strain A118

40.426

89.891

0.363
  pilT Acinetobacter baumannii D1279779

40.426

89.891

0.363
  pilT Acinetobacter nosocomialis M2

40.122

89.891

0.361

Multiple sequence alignment