Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6M93_RS00865 Genome accession   NZ_CP170503
Coordinates   185194..186210 (+) Length   338 a.a.
NCBI ID   WP_039465942.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain BSRLOP2CAB     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180194..191210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6M93_RS00835 (V6M93_00835) hemW 180908..182050 (-) 1143 WP_039526648.1 radical SAM family heme chaperone HemW -
  V6M93_RS00840 (V6M93_00840) - 182043..182636 (-) 594 WP_014916538.1 XTP/dITP diphosphatase -
  V6M93_RS00845 (V6M93_00845) yggU 182654..182944 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  V6M93_RS00850 (V6M93_00850) - 182941..183495 (-) 555 WP_039521477.1 YggT family protein -
  V6M93_RS00855 (V6M93_00855) proC 183544..184365 (-) 822 WP_039521701.1 pyrroline-5-carboxylate reductase -
  V6M93_RS00860 (V6M93_00860) - 184460..185173 (-) 714 WP_039465940.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6M93_RS00865 (V6M93_00865) pilT 185194..186210 (+) 1017 WP_039465942.1 type IV pilus twitching motility protein PilT Machinery gene
  V6M93_RS00870 (V6M93_00870) - 186179..188011 (-) 1833 WP_039521474.1 diguanylate cyclase -
  V6M93_RS00875 (V6M93_00875) - 188541..190112 (-) 1572 WP_040038285.1 methyl-accepting chemotaxis protein -
  V6M93_RS00880 (V6M93_00880) nsrR 190456..190881 (+) 426 WP_040038284.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36642.86 Da        Isoelectric Point: 6.4934

>NTDB_id=1057798 V6M93_RS00865 WP_039465942.1 185194..186210(+) (pilT) [Pectobacterium brasiliense strain BSRLOP2CAB]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLTTLQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRCCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1057798 V6M93_RS00865 WP_039465942.1 185194..186210(+) (pilT) [Pectobacterium brasiliense strain BSRLOP2CAB]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCAGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCCGCTCAGCGCGAGCAACTACGGCAGGCTGGGCAGGTAGATGGGGCATTAATGCTGCCTGATGGACAA
CGCCTGCGGGTGAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTGTTATTCCGTCCGTTCAGCCTTCATT
GACGACGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGATGTTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGCGCATCGTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGGGAATTGCGTGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTATCCACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCAGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACTTGTTTGCAGGCGGTAGTGACGCA
AAAACTGCTGCCCGCTGCGCAAGGTGGCCGAATCGCGCTTTATGAAGTGCTGACGGCGACTGCGGCGGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAACGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment