Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFIOZ_RS01920 Genome accession   NZ_CP170448
Coordinates   391816..392859 (+) Length   347 a.a.
NCBI ID   WP_386081869.1    Uniprot ID   -
Organism   Vreelandella sp. F11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386816..397859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOZ_RS01890 (ACFIOZ_01890) - 387142..387591 (-) 450 WP_386081862.1 DUF4426 domain-containing protein -
  ACFIOZ_RS01895 (ACFIOZ_01895) metW 387633..388226 (-) 594 WP_088702231.1 methionine biosynthesis protein MetW -
  ACFIOZ_RS01900 (ACFIOZ_01900) metX 388226..389452 (-) 1227 WP_386081865.1 homoserine O-succinyltransferase MetX -
  ACFIOZ_RS01905 (ACFIOZ_01905) - 389539..390132 (-) 594 WP_088702216.1 YggT family protein -
  ACFIOZ_RS01910 (ACFIOZ_01910) proC 390187..391011 (-) 825 WP_386081867.1 pyrroline-5-carboxylate reductase -
  ACFIOZ_RS01915 (ACFIOZ_01915) - 391067..391783 (-) 717 WP_386081868.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFIOZ_RS01920 (ACFIOZ_01920) pilT 391816..392859 (+) 1044 WP_386081869.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFIOZ_RS01925 (ACFIOZ_01925) - 392898..394037 (-) 1140 WP_386081871.1 anhydro-N-acetylmuramic acid kinase -
  ACFIOZ_RS01930 (ACFIOZ_01930) tyrS 394177..395376 (+) 1200 WP_386081873.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38170.77 Da        Isoelectric Point: 6.1841

>NTDB_id=1057486 ACFIOZ_RS01920 WP_386081869.1 391816..392859(+) (pilT) [Vreelandella sp. F11]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYAIMNDRQRRDYEEHLEIDFSFEVPGVAR
FRVNAFTQARGAGAVFRTIPNQVLSMQALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHMSKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAVKTIDRI
IDVFPGEERAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDAAL
TRLVKEGTVSLDEAQLSAKGALALKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1057486 ACFIOZ_RS01920 WP_386081869.1 391816..392859(+) (pilT) [Vreelandella sp. F11]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTACCACCCAT
GATACGTGTTGATGGCGACATTCGTCGGCTCAATGTGCCCGCTATCGATAATAATGATGTTCGCAAATTGATCTACGCCA
TCATGAATGATCGACAGCGGCGCGACTACGAAGAGCACCTGGAAATTGATTTTTCCTTTGAAGTCCCTGGGGTAGCTCGC
TTTCGTGTTAACGCGTTCACCCAGGCCCGCGGGGCGGGGGCGGTATTTCGTACCATTCCCAATCAAGTGCTCTCCATGCA
AGCCCTGGGGCTCGGCGAGGTGTTCGAGCGTCTGGCGATGTTACCGCGTGGCCTGGTGCTGGTGACAGGGCCAACGGGGT
CGGGTAAGAGCACCACGTTGGCCGCGATGATCGACTACATTAACGACCACCGCTACGAGCACATTCTGACCATCGAAGAC
CCGGTTGAGTTTGTTCACATGAGCAAGCGCTGCTTGATTAACCAGCGCGAAGTGCATCGCGATACCCATAGCTTTGCCGA
CGCGTTACGTAGTGCGTTGCGTGAAGACCCGGATGTGATTCTGGTCGGTGAGCTGCGCGATCTGGAAACCATTCGATTAG
CGCTGACCGCCGCGGAGACAGGTCACTTGGTGCTGGGAACGCTGCATACCACCTCTGCCGTCAAGACAATCGACCGGATC
ATCGATGTTTTTCCCGGCGAAGAGAGAGCCATGGTGCGCTCCATGCTGTCGGAGTCACTCCAGGCAGTGGTATCGCAATC
GCTGCTTAAACGACAGGGGGGCGGACGCGTAGCGGCCCATGAAATCCTCATTGCCACTTCTGCAGTGCGCAACTTAATCC
GCGAAGACAAAGTGGCGCAGATGTACTCGGCCATTCAAACCGGCGGCAGCCTGGGCATGCAAACCCTGGATGCCGCGCTT
ACTCGGTTAGTGAAAGAGGGTACGGTAAGTTTGGATGAGGCGCAGCTGAGTGCGAAGGGAGCGCTGGCATTGAAAGATGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.286

97.695

0.755
  pilT Pseudomonas aeruginosa PAK

77.286

97.695

0.755
  pilT Acinetobacter nosocomialis M2

75.221

97.695

0.735
  pilT Acinetobacter baumannii D1279779

75.221

97.695

0.735
  pilT Acinetobacter baumannii strain A118

75.221

97.695

0.735
  pilT Acinetobacter baylyi ADP1

74.336

97.695

0.726
  pilT Legionella pneumophila strain Lp02

71.893

97.406

0.7
  pilT Legionella pneumophila strain ERS1305867

71.893

97.406

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.851

96.542

0.674
  pilT Vibrio cholerae strain A1552

69.851

96.542

0.674
  pilT Neisseria meningitidis 8013

64.62

98.559

0.637
  pilT Neisseria gonorrhoeae MS11

63.768

99.424

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.627

96.542

0.527
  pilU Pseudomonas stutzeri DSM 10701

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

41.618

99.712

0.415
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395

Multiple sequence alignment