Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BCU28_RS14620 Genome accession   NZ_CP170022
Coordinates   3268725..3269762 (-) Length   345 a.a.
NCBI ID   WP_016791042.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.45.B7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3263725..3274762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU28_RS14590 (BCU28_14590) rsmE 3264431..3265162 (+) 732 WP_016783757.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BCU28_RS14595 (BCU28_14595) gshB 3265172..3266122 (+) 951 WP_004735482.1 glutathione synthase -
  BCU28_RS14600 (BCU28_14600) - 3266239..3266802 (+) 564 WP_016791041.1 YqgE/AlgH family protein -
  BCU28_RS14605 (BCU28_14605) ruvX 3266862..3267284 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  BCU28_RS14610 (BCU28_14610) - 3267383..3267544 (+) 162 WP_016785941.1 hypothetical protein -
  BCU28_RS14615 (BCU28_14615) pilU 3267601..3268710 (-) 1110 WP_010435664.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BCU28_RS14620 (BCU28_14620) pilT 3268725..3269762 (-) 1038 WP_016791042.1 type IV pilus twitching motility protein PilT Machinery gene
  BCU28_RS14625 (BCU28_14625) - 3269789..3270493 (+) 705 WP_010435657.1 YggS family pyridoxal phosphate-dependent enzyme -
  BCU28_RS14630 (BCU28_14630) proC 3270606..3271424 (+) 819 WP_010435654.1 pyrroline-5-carboxylate reductase -
  BCU28_RS14635 (BCU28_14635) - 3271501..3272058 (+) 558 WP_010435653.1 YggT family protein -
  BCU28_RS14640 (BCU28_14640) yggU 3272088..3272378 (+) 291 WP_010435651.1 DUF167 family protein YggU -
  BCU28_RS14645 (BCU28_14645) - 3272409..3272840 (+) 432 WP_010435648.1 DUF4426 domain-containing protein -
  BCU28_RS14650 (BCU28_14650) - 3272889..3273488 (+) 600 WP_010435646.1 XTP/dITP diphosphatase -
  BCU28_RS14655 (BCU28_14655) hemW 3273488..3274675 (+) 1188 WP_010435644.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38192.73 Da        Isoelectric Point: 6.0495

>NTDB_id=1054709 BCU28_RS14620 WP_016791042.1 3268725..3269762(-) (pilT) [Vibrio cyclitrophicus strain 10N.286.45.B7]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRLACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGLVDSDEVEKKIEIQASMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1054709 BCU28_RS14620 WP_016791042.1 3268725..3269762(-) (pilT) [Vibrio cyclitrophicus strain 10N.286.45.B7]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTACATCGTTTAGTTTTTGAGA
TCATGGACGATTCACAACGCGGTGAGTTTGAGGAAAAGTTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTTAATGCTTTTAATCAAGCTCGTGGGTGTGCTGCTGTCTTTCGAACTATCCCTGTAGAGATCCCGACTTTAGA
TCAGCTTGGTGCGCCAGAAATCTTTGAAAAGATTGCGAACTACGAGAAAGGTTTAGTGCTTGTCACTGGCCCTACAGGTT
CTGGTAAATCGACAACTTTGGCGGCGATGGTGGATTACGTAAACCGCAATCACAACAAACACATTTTGACGATTGAAGAT
CCAATTGAATTCGTTCACACCAATAATAAATGCCTAGTAAACCAGCGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGTTGCGCAGTGCATTACGTGAGGATCCGGACGTGATTCTTGTTGGTGAACTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACCGCAGCTGAAACGGGTCACTTAGTTTTTGGTACTTTGCATACAAGCTCTGCGGCAAAAACTATCGACCGTATT
ATCGACGTGTTCCCAGGTAGCGACAAAGATATGGTTCGTTCAATGTTATCTGAATCACTACGCTCGGTGATTGCTCAGAA
GCTGTTAAAACGTGTTGGTGGTGGTCGTTTGGCTTGTCATGAAATAATGATGGCGACACCTGCAATTAGAAACTTGATCC
GTGAAGACAAAGTCGCGCAGATGTACTCGATCATTCAAACGGGTGCCGCACATGGCATGCAAACCATGGAGCAAAATGCG
AAGCAATTGATCGCGCAAGGTTTGGTTGATTCGGACGAGGTCGAGAAAAAGATCGAAATTCAAGCCTCTATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.34

95.362

0.69
  pilT Acinetobacter baumannii strain A118

72.34

95.362

0.69
  pilT Acinetobacter nosocomialis M2

72.036

95.362

0.687
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Pseudomonas aeruginosa PAK

68.235

98.551

0.672
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

62.319

100

0.623
  pilT Neisseria gonorrhoeae MS11

62.029

100

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.159

95.072

0.391
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment