Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BCU99_RS13160 Genome accession   NZ_CP170015
Coordinates   2955187..2956224 (-) Length   345 a.a.
NCBI ID   WP_010435660.1    Uniprot ID   A0AAN0LU04
Organism   Vibrio cyclitrophicus strain 10N.286.52.F6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2950187..2961224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU99_RS13130 (BCU99_13130) rsmE 2950904..2951635 (+) 732 WP_016783757.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BCU99_RS13135 (BCU99_13135) gshB 2951645..2952595 (+) 951 WP_004735482.1 glutathione synthase -
  BCU99_RS13140 (BCU99_13140) - 2952712..2953275 (+) 564 WP_016768176.1 YqgE/AlgH family protein -
  BCU99_RS13145 (BCU99_13145) ruvX 2953324..2953746 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  BCU99_RS13150 (BCU99_13150) - 2953845..2954006 (+) 162 WP_016785941.1 hypothetical protein -
  BCU99_RS13155 (BCU99_13155) pilU 2954063..2955172 (-) 1110 WP_010435664.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BCU99_RS13160 (BCU99_13160) pilT 2955187..2956224 (-) 1038 WP_010435660.1 type IV pilus twitching motility protein PilT Machinery gene
  BCU99_RS13165 (BCU99_13165) - 2956251..2956955 (+) 705 WP_010435657.1 YggS family pyridoxal phosphate-dependent enzyme -
  BCU99_RS13170 (BCU99_13170) proC 2957068..2957886 (+) 819 WP_010435654.1 pyrroline-5-carboxylate reductase -
  BCU99_RS13175 (BCU99_13175) - 2957963..2958520 (+) 558 WP_010435653.1 YggT family protein -
  BCU99_RS13180 (BCU99_13180) yggU 2958550..2958840 (+) 291 WP_010435651.1 DUF167 family protein YggU -
  BCU99_RS13185 (BCU99_13185) - 2958871..2959302 (+) 432 WP_016768173.1 DUF4426 domain-containing protein -
  BCU99_RS13190 (BCU99_13190) - 2959351..2959950 (+) 600 WP_010435646.1 XTP/dITP diphosphatase -
  BCU99_RS13195 (BCU99_13195) hemW 2959950..2961137 (+) 1188 WP_016788326.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38178.70 Da        Isoelectric Point: 6.0495

>NTDB_id=1054572 BCU99_RS13160 WP_010435660.1 2955187..2956224(-) (pilT) [Vibrio cyclitrophicus strain 10N.286.52.F6]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGLVDSDEVEKKIEIQASMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1054572 BCU99_RS13160 WP_010435660.1 2955187..2956224(-) (pilT) [Vibrio cyclitrophicus strain 10N.286.52.F6]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTACATCGTTTAGTTTTTGAGA
TCATGGACGATTCACAACGCGGTGAGTTTGAGGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTTAATGCTTTTAATCAAGCTCGTGGGTGTGCTGCTGTCTTTCGAACTATCCCTGTAGAGATCCCGACTTTAGA
TCAGCTTGGTGCGCCAGAAATCTTTGAAAAGATTGCGAACTACGAGAAAGGTTTAGTGCTTGTCACTGGCCCTACAGGTT
CTGGTAAATCGACAACTTTGGCGGCGATGGTGGATTACGTAAACCGCAATCACAACAAACACATTTTGACGATTGAAGAT
CCAATTGAATTCGTTCACACCAATAATAAATGCCTAGTAAACCAGCGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGTTGCGCAGTGCATTACGTGAGGATCCGGACGTGATTCTTGTTGGTGAACTTCGTGACCAAGAGACGATCAGCTTAG
CGTTAACCGCAGCTGAAACGGGTCACTTAGTTTTTGGTACTTTGCATACAAGCTCTGCGGCAAAAACTATCGACCGTATT
ATCGACGTTTTCCCAGGTAGCGACAAAGATATGGTTCGTTCAATGTTATCTGAATCACTACGCTCGGTGATTGCTCAAAA
GCTGTTAAAGCGTGTTGGTGGTGGTCGTGTGGCTTGTCATGAAATAATGATGGCGACACCTGCAATTAGAAACTTGATCC
GTGAAGACAAAGTCGCGCAGATGTACTCGATCATTCAAACGGGTGCCGCACATGGCATGCAAACCATGGAGCAAAATGCG
AAGCAATTGATCGCGCAAGGTTTGGTTGATTCGGACGAGGTCGAGAAAAAGATCGAAATTCAAGCCTCTATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

72.644

95.362

0.693
  pilT Acinetobacter baumannii strain A118

72.644

95.362

0.693
  pilT Acinetobacter nosocomialis M2

72.34

95.362

0.69
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Legionella pneumophila strain Lp02

68.085

95.362

0.649
  pilT Legionella pneumophila strain ERS1305867

68.085

95.362

0.649
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

98.841

0.499
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.159

95.072

0.391
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment