Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACETK2_RS17475 Genome accession   NZ_CP169640
Coordinates   3897349..3898365 (-) Length   338 a.a.
NCBI ID   WP_015841590.1    Uniprot ID   C6DE29
Organism   Pectobacterium aroidearum strain Pc3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3892349..3903365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACETK2_RS17460 nsrR 3892712..3893137 (-) 426 WP_015841587.1 nitric oxide-sensing transcriptional repressor NsrR -
  ACETK2_RS17465 - 3893454..3895025 (+) 1572 WP_015841588.1 methyl-accepting chemotaxis protein -
  ACETK2_RS17470 - 3895554..3897380 (+) 1827 WP_015841589.1 diguanylate cyclase -
  ACETK2_RS17475 pilT 3897349..3898365 (-) 1017 WP_015841590.1 type IV pilus twitching motility protein PilT Machinery gene
  ACETK2_RS17480 - 3898387..3899100 (+) 714 WP_015841591.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACETK2_RS17485 proC 3899191..3900012 (+) 822 WP_015841592.1 pyrroline-5-carboxylate reductase -
  ACETK2_RS17490 - 3900061..3900615 (+) 555 WP_010295003.1 YggT family protein -
  ACETK2_RS17495 yggU 3900612..3900902 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  ACETK2_RS17500 - 3900920..3901513 (+) 594 WP_015841593.1 XTP/dITP diphosphatase -
  ACETK2_RS17505 hemW 3901506..3902648 (+) 1143 WP_015841594.1 radical SAM family heme chaperone HemW -
  ACETK2_RS17510 - 3902877..3903137 (+) 261 WP_015841595.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36914.20 Da        Isoelectric Point: 7.2808

>NTDB_id=1052550 ACETK2_RS17475 WP_015841590.1 3897349..3898365(-) (pilT) [Pectobacterium aroidearum strain Pc3]
MELDKWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSEQVARWCDAWLEPAQREQLRQFGQIDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDGLHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRATLREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLSTCLQAVVTQRLLPSAHGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHNCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1052550 ACETK2_RS17475 WP_015841590.1 3897349..3898365(-) (pilT) [Pectobacterium aroidearum strain Pc3]
ATGGAGTTGGATAAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCAGATCTGCACCTTTGTAGCGGCCATCCGCCCGT
GTTACGTGTCGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGAGCAGGTGGCACGATGGTGTGATG
CCTGGCTTGAACCCGCTCAGAGAGAACAACTGCGGCAGTTTGGTCAGATAGACGGCGCGTTAATGCTGCCTGATGGGCAG
CGTCTGCGGGTAAATGTGTTTCGCCAGCGAGAAGGGCTGTCCGCGGCGCTGCGTATTATCCCGTCCGTTCAGCCGTCATT
GGACGGGCTACACGCGCCACCCGTTCTGTCGACGCTGTTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCAATGCTCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGAGAGATCGGTGCGCACAGCGCGTCTTTTGC
ACAGGCGCTACGGGCCACCTTGCGGGAAGATCCCGATGTGATTTTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGTCATCTGGTGCTATCCACGCTGCATACGCGCAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGTCAGCTATCCACCTGCTTGCAGGCCGTGGTGACGCA
AAGGTTGTTGCCTTCTGCACACGGCGGTCGTATTGCGCTTTATGAAGTGCTGACGGCGACAGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCATCAGCTTCCCGGCCTGATTCAAACGGGAACGGCTGCGGGCATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGATGTCGGGATGGGCTTATTTCACACAATTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6DE29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

99.408

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

97.633

0.497
  pilT Vibrio cholerae strain A1552

50.909

97.633

0.497
  pilT Neisseria gonorrhoeae MS11

49.702

99.408

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.955

99.112

0.485
  pilT Legionella pneumophila strain ERS1305867

48.657

99.112

0.482
  pilT Legionella pneumophila strain Lp02

48.657

99.112

0.482
  pilT Pseudomonas aeruginosa PAK

48.657

99.112

0.482
  pilT Acinetobacter baylyi ADP1

48.225

100

0.482
  pilT Acinetobacter nosocomialis M2

48.06

99.112

0.476
  pilT Acinetobacter baumannii D1279779

48.06

99.112

0.476
  pilT Acinetobacter baumannii strain A118

48.06

99.112

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.378

97.041

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.246

99.704

0.411
  pilU Vibrio cholerae strain A1552

39.466

99.704

0.393
  pilU Acinetobacter baylyi ADP1

39.13

95.266

0.373

Multiple sequence alignment