Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEY3W_RS03000 Genome accession   NZ_CP169333
Coordinates   615724..616704 (+) Length   326 a.a.
NCBI ID   WP_001055642.1    Uniprot ID   A0A3V9WZJ6
Organism   Salmonella enterica strain SA99     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 610724..621704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY3W_RS02970 (ACEY3W_02970) - 611301..612308 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  ACEY3W_RS02975 (ACEY3W_02975) hemW 612400..613536 (-) 1137 WP_023138753.1 radical SAM family heme chaperone HemW -
  ACEY3W_RS02980 (ACEY3W_02980) - 613529..614122 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  ACEY3W_RS02985 (ACEY3W_02985) yggU 614130..614420 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  ACEY3W_RS02990 (ACEY3W_02990) - 614417..614983 (-) 567 WP_001094848.1 YggT family protein -
  ACEY3W_RS02995 (ACEY3W_02995) - 615002..615706 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEY3W_RS03000 (ACEY3W_03000) pilT 615724..616704 (+) 981 WP_001055642.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEY3W_RS03005 (ACEY3W_03005) - 616834..617520 (+) 687 WP_023202165.1 IclR family transcriptional regulator -
  ACEY3W_RS03010 (ACEY3W_03010) ruvX 617567..617983 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  ACEY3W_RS03015 (ACEY3W_03015) - 617983..618546 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  ACEY3W_RS03020 (ACEY3W_03020) gshB 618762..619709 (-) 948 WP_023232636.1 glutathione synthase -
  ACEY3W_RS03025 (ACEY3W_03025) rsmE 619729..620460 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEY3W_RS03030 (ACEY3W_03030) endA 620537..621244 (-) 708 WP_023232637.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36125.28 Da        Isoelectric Point: 7.5355

>NTDB_id=1050638 ACEY3W_RS03000 WP_001055642.1 615724..616704(+) (pilT) [Salmonella enterica strain SA99]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1050638 ACEY3W_RS03000 WP_001055642.1 615724..616704(+) (pilT) [Salmonella enterica strain SA99]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCACCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V9WZJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment