Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEPA4_RS02640 Genome accession   NZ_CP169216
Coordinates   561682..562809 (-) Length   375 a.a.
NCBI ID   WP_049416904.1    Uniprot ID   A0AAP7J565
Organism   Stenotrophomonas sp. P2112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 556682..567809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPA4_RS02620 (ACEPA4_02620) - 557113..558102 (+) 990 WP_374082980.1 fumarylacetoacetate hydrolase family protein -
  ACEPA4_RS02625 (ACEPA4_02625) maiA 558117..558785 (+) 669 WP_374082981.1 maleylacetoacetate isomerase -
  ACEPA4_RS02630 (ACEPA4_02630) - 558829..560289 (-) 1461 WP_374082982.1 UdgX family uracil-DNA binding protein -
  ACEPA4_RS02635 (ACEPA4_02635) - 560270..561553 (-) 1284 WP_069139423.1 putative DNA modification/repair radical SAM protein -
  ACEPA4_RS02640 (ACEPA4_02640) pilU 561682..562809 (-) 1128 WP_049416904.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPA4_RS02645 (ACEPA4_02645) - 562931..563296 (+) 366 WP_005415160.1 DUF4398 domain-containing protein -
  ACEPA4_RS02650 (ACEPA4_02650) - 563293..564177 (+) 885 WP_374082983.1 hypothetical protein -
  ACEPA4_RS02655 (ACEPA4_02655) - 564502..564738 (+) 237 WP_005407964.1 YdcH family protein -
  ACEPA4_RS02660 (ACEPA4_02660) - 565039..566409 (+) 1371 WP_353089389.1 pyridoxal-phosphate dependent enzyme -
  ACEPA4_RS02665 (ACEPA4_02665) - 566533..567714 (+) 1182 WP_374082984.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41398.70 Da        Isoelectric Point: 6.3741

>NTDB_id=1049825 ACEPA4_RS02640 WP_049416904.1 561682..562809(-) (pilU) [Stenotrophomonas sp. P2112]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1049825 ACEPA4_RS02640 WP_049416904.1 561682..562809(-) (pilU) [Stenotrophomonas sp. P2112]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAAGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCTTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCGCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTACTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGTAGCGGCGAGCACGACCCGTACGCCGATTTCTCGGCAGGCGCACCCAGCATCACTCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment