Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEUKD_RS12740 Genome accession   NZ_CP169050
Coordinates   2740386..2741492 (-) Length   368 a.a.
NCBI ID   WP_006743091.1    Uniprot ID   A0AAE4M7Q6
Organism   Vibrio diabolicus strain SF42     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735386..2746492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEUKD_RS12710 (ACEUKD_12710) - 2736017..2736514 (+) 498 WP_046875108.1 SprT family zinc-dependent metalloprotease -
  ACEUKD_RS12715 (ACEUKD_12715) endA 2736665..2737360 (+) 696 WP_047008832.1 deoxyribonuclease I -
  ACEUKD_RS12720 (ACEUKD_12720) rsmE 2737477..2738208 (+) 732 WP_374093176.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEUKD_RS12725 (ACEUKD_12725) gshB 2738222..2739172 (+) 951 WP_006743090.1 glutathione synthase -
  ACEUKD_RS12730 (ACEUKD_12730) - 2739301..2739864 (+) 564 WP_005393973.1 YqgE/AlgH family protein -
  ACEUKD_RS12735 (ACEUKD_12735) ruvX 2739899..2740324 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  ACEUKD_RS12740 (ACEUKD_12740) pilU 2740386..2741492 (-) 1107 WP_006743091.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEUKD_RS12745 (ACEUKD_12745) pilT 2741513..2742553 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEUKD_RS12750 (ACEUKD_12750) - 2742582..2743283 (+) 702 WP_046875110.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEUKD_RS12755 (ACEUKD_12755) proC 2743441..2744259 (+) 819 WP_374093175.1 pyrroline-5-carboxylate reductase -
  ACEUKD_RS12760 (ACEUKD_12760) - 2744312..2744869 (+) 558 WP_012841393.1 YggT family protein -
  ACEUKD_RS12765 (ACEUKD_12765) yggU 2744869..2745159 (+) 291 WP_374093174.1 DUF167 family protein YggU -
  ACEUKD_RS12770 (ACEUKD_12770) - 2745287..2745718 (+) 432 WP_005393982.1 DUF4426 domain-containing protein -
  ACEUKD_RS12775 (ACEUKD_12775) - 2745837..2746439 (+) 603 WP_085342619.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41253.38 Da        Isoelectric Point: 6.7734

>NTDB_id=1049558 ACEUKD_RS12740 WP_006743091.1 2740386..2741492(-) (pilU) [Vibrio diabolicus strain SF42]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1049558 ACEUKD_RS12740 WP_006743091.1 2740386..2741492(-) (pilU) [Vibrio diabolicus strain SF42]
ATGGATCTAAATAAATTTCTCGAAGGTATGCTGGCGCTGAAAGCGTCGGATCTGTACATCACGGTTGGTGCGCCAATTTT
GTTTCGCGTGGATGGCGAATTGCGACCTCAAGGTGAAAAACTCACCGAGAGTGATGTGGCGGCATTACTCGACAGTGCTA
TGGAGCCAGATCGGCGTCAGGAATTTCGTAAAAGTCGTGAATCGAATTTTGCCATTGTGAGAGATTGTGGCCGCTTCCGT
GTTAGTGCTTTTTTTCAACGGGAATTACCGGGTGCGGTGATTCGTCGTATTGAAACCAATATTCCCACCTTTGAGCAATT
AAAGTTACCGTTAGTGTTGCAAGATTTAGCGATAGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCTACTGGGTCAGGTA
AGTCCACCACAATGGCGGCAATGACGGGCTATCGAAACAGCAACAAGACGGGGCACATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAGCATAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGTCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGCCATTTGTGTATGGCAACGTTGCACGCCAACAATGCCAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGTGTGGTTGGTCAGCAGCTGAT
CCGAGACAAAAATGGGCAGGGGCGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATCCGTC
GCGGAGATTTGCATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGACGCGCTGCACAGTGCCGATTCGGCGAATGATTTGCGCTTGATGTT
GAAAACACAGCGTGGCGAAGCATTCTCTACGGGCAGTCTTGCTAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment