Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACES2N_RS20045 Genome accession   NZ_CP168973
Coordinates   3791023..3792123 (+) Length   366 a.a.
NCBI ID   WP_088563811.1    Uniprot ID   A0A1Z3N3T8
Organism   Bdellovibrio bacteriovorus strain HK2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3786023..3797123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACES2N_RS20025 (ACES2N_20025) rpmA 3786587..3786847 (-) 261 WP_011166148.1 50S ribosomal protein L27 -
  ACES2N_RS20030 (ACES2N_20030) rplU 3786871..3787437 (-) 567 WP_374031586.1 50S ribosomal protein L21 -
  ACES2N_RS20035 (ACES2N_20035) - 3787644..3789245 (-) 1602 WP_374031587.1 hypothetical protein -
  ACES2N_RS20040 (ACES2N_20040) - 3789360..3790874 (-) 1515 Protein_3962 ribonuclease E/G -
  ACES2N_RS20045 (ACES2N_20045) pilT 3791023..3792123 (+) 1101 WP_088563811.1 type IV pilus twitching motility protein PilT Machinery gene
  ACES2N_RS20050 (ACES2N_20050) - 3792125..3792502 (-) 378 WP_232468932.1 hypothetical protein -
  ACES2N_RS20055 (ACES2N_20055) deoC 3792562..3793224 (+) 663 WP_374031588.1 deoxyribose-phosphate aldolase -
  ACES2N_RS20060 (ACES2N_20060) - 3793224..3794543 (+) 1320 WP_374031589.1 thymidine phosphorylase -
  ACES2N_RS20065 (ACES2N_20065) - 3794598..3795387 (+) 790 Protein_3967 purine-nucleoside phosphorylase -
  ACES2N_RS20070 (ACES2N_20070) - 3795532..3796761 (+) 1230 WP_374031590.1 S8 family peptidase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 39794.96 Da        Isoelectric Point: 7.9844

>NTDB_id=1049061 ACES2N_RS20045 WP_088563811.1 3791023..3792123(+) (pilT) [Bdellovibrio bacteriovorus strain HK2]
MATIDELFKLMVEQGASDLHITSGAAPYLRLHGNMVPLNYRELTNQDVQGLLFEILSEKQKKAFVEKWELDFAYTLSGIG
RFRCNIFMQRKGLGAVMRIIPEKIKTAQELGLPPAVMDMIDCDRGLILVTGPTGSGKSTTLAAMIHQINMTREAHIITVE
DPIEFVHPNLKSLVNQREVGSHTKSFANALKAALREDPDILLVGELRDLETISLALTAAETGHIVFGTLHTNSAAKTIDR
IIDVFPAGQQQQIRTMLAESLRGVVAQTLFSRADGQGRVAAYEIMRNTKAISNLVREGKVHQIPSAMQTGATQGMVLFEK
YIEDLVRKGKVSAADAKTFLGQAGGGDTSVQPGATMAGGTPRTKVG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1049061 ACES2N_RS20045 WP_088563811.1 3791023..3792123(+) (pilT) [Bdellovibrio bacteriovorus strain HK2]
ATGGCAACAATTGATGAATTGTTCAAACTCATGGTGGAACAAGGTGCCTCCGACTTGCATATCACAAGTGGAGCCGCCCC
TTATTTGCGCCTGCATGGAAACATGGTTCCATTGAACTATCGCGAACTCACGAATCAAGACGTACAGGGACTGCTGTTTG
AGATTCTTTCCGAAAAGCAAAAGAAAGCCTTCGTTGAAAAATGGGAGCTGGATTTCGCCTACACTTTGTCAGGTATCGGG
CGTTTCCGTTGCAATATCTTTATGCAGCGTAAAGGCCTTGGTGCCGTTATGCGTATCATCCCGGAAAAAATCAAAACCGC
ACAAGAGCTGGGACTGCCTCCGGCCGTGATGGACATGATCGATTGCGATCGTGGTCTGATTCTTGTCACCGGTCCGACAG
GTTCTGGTAAGTCCACAACTCTGGCGGCGATGATTCACCAAATTAACATGACCCGTGAAGCCCACATCATCACGGTGGAA
GACCCGATCGAGTTCGTTCATCCGAATTTGAAATCCCTGGTCAATCAGCGTGAGGTCGGAAGCCACACCAAAAGTTTTGC
CAACGCCCTGAAAGCGGCCTTGCGTGAAGACCCGGATATTTTGCTGGTGGGTGAGCTTCGTGACCTAGAAACAATTTCTT
TGGCACTGACTGCTGCCGAAACAGGTCATATCGTTTTCGGAACCCTGCACACCAACAGTGCGGCGAAAACCATCGATCGT
ATCATTGACGTATTCCCTGCCGGTCAACAGCAACAGATCCGAACGATGCTGGCTGAATCCCTGCGCGGAGTTGTCGCTCA
GACCCTATTCTCCCGCGCGGACGGACAAGGTCGTGTGGCGGCTTACGAGATCATGAGAAACACCAAAGCCATCTCGAACT
TGGTTCGTGAAGGTAAAGTCCACCAGATCCCATCTGCCATGCAGACCGGTGCCACTCAAGGGATGGTTCTGTTTGAAAAG
TACATTGAGGATCTGGTTCGTAAGGGTAAAGTTTCCGCAGCCGATGCCAAGACCTTCCTGGGTCAGGCCGGTGGTGGCGA
CACCAGCGTACAGCCAGGGGCGACCATGGCTGGCGGCACCCCACGCACCAAAGTGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N3T8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.515

99.18

0.511
  pilT Acinetobacter nosocomialis M2

54.627

91.53

0.5
  pilT Acinetobacter baumannii D1279779

54.627

91.53

0.5
  pilT Acinetobacter baumannii strain A118

54.627

91.53

0.5
  pilT Acinetobacter baylyi ADP1

54.491

91.257

0.497
  pilT Pseudomonas aeruginosa PAK

54.491

91.257

0.497
  pilT Vibrio cholerae strain A1552

54.03

91.53

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.03

91.53

0.495
  pilT Pseudomonas stutzeri DSM 10701

53.892

91.257

0.492
  pilT Legionella pneumophila strain ERS1305867

54.407

89.891

0.489
  pilT Legionella pneumophila strain Lp02

54.407

89.891

0.489
  pilT Neisseria gonorrhoeae MS11

50.599

91.257

0.462
  pilT Neisseria meningitidis 8013

50.599

91.257

0.462
  pilU Pseudomonas stutzeri DSM 10701

41.84

92.077

0.385
  pilU Acinetobacter baylyi ADP1

41.018

91.257

0.374
  pilU Vibrio cholerae strain A1552

41.104

89.071

0.366

Multiple sequence alignment