Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACERHX_RS03745 Genome accession   NZ_CP168932
Coordinates   779750..780730 (+) Length   326 a.a.
NCBI ID   WP_001660979.1    Uniprot ID   A0A5W9F825
Organism   Salmonella enterica strain SA45     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774750..785730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACERHX_RS03715 (ACERHX_03715) - 775327..776334 (+) 1008 WP_001524027.1 DUF1202 domain-containing protein -
  ACERHX_RS03720 (ACERHX_03720) hemW 776426..777562 (-) 1137 WP_076741765.1 radical SAM family heme chaperone HemW -
  ACERHX_RS03725 (ACERHX_03725) - 777555..778148 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  ACERHX_RS03730 (ACERHX_03730) yggU 778156..778446 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  ACERHX_RS03735 (ACERHX_03735) - 778443..779009 (-) 567 WP_001094848.1 YggT family protein -
  ACERHX_RS03740 (ACERHX_03740) - 779028..779732 (-) 705 WP_000997787.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACERHX_RS03745 (ACERHX_03745) pilT 779750..780730 (+) 981 WP_001660979.1 type IV pilus twitching motility protein PilT Machinery gene
  ACERHX_RS03750 (ACERHX_03750) - 780863..781549 (+) 687 WP_023243685.1 IclR family transcriptional regulator -
  ACERHX_RS03755 (ACERHX_03755) ruvX 781595..782011 (-) 417 WP_001014571.1 Holliday junction resolvase RuvX -
  ACERHX_RS03760 (ACERHX_03760) - 782011..782574 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  ACERHX_RS03765 (ACERHX_03765) gshB 782790..783737 (-) 948 WP_000593248.1 glutathione synthase -
  ACERHX_RS03770 (ACERHX_03770) rsmE 783757..784488 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACERHX_RS03775 (ACERHX_03775) endA 784565..785272 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36153.38 Da        Isoelectric Point: 7.8163

>NTDB_id=1048851 ACERHX_RS03745 WP_001660979.1 779750..780730(+) (pilT) [Salmonella enterica strain SA45]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRKHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREVGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1048851 ACERHX_RS03745 WP_001660979.1 779750..780730(+) (pilT) [Salmonella enterica strain SA45]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAAAGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAGTCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACACCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5W9F825

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

46.203

96.933

0.448
  pilT Pseudomonas stutzeri DSM 10701

46.203

96.933

0.448
  pilT Acinetobacter nosocomialis M2

45.768

97.853

0.448
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment