Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACERHU_RS03840 Genome accession   NZ_CP168929
Coordinates   795381..796361 (+) Length   326 a.a.
NCBI ID   WP_001619985.1    Uniprot ID   -
Organism   Salmonella enterica strain SA136     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 790381..801361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACERHU_RS03810 (ACERHU_03810) - 790959..791966 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  ACERHU_RS03815 (ACERHU_03815) hemW 792057..793193 (-) 1137 WP_023209130.1 radical SAM family heme chaperone HemW -
  ACERHU_RS03820 (ACERHU_03820) - 793186..793779 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  ACERHU_RS03825 (ACERHU_03825) yggU 793787..794077 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  ACERHU_RS03830 (ACERHU_03830) - 794074..794640 (-) 567 WP_001094848.1 YggT family protein -
  ACERHU_RS03835 (ACERHU_03835) - 794659..795363 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACERHU_RS03840 (ACERHU_03840) pilT 795381..796361 (+) 981 WP_001619985.1 type IV pilus twitching motility protein PilT Machinery gene
  ACERHU_RS03845 (ACERHU_03845) - 796494..797180 (+) 687 WP_023243685.1 IclR family transcriptional regulator -
  ACERHU_RS03850 (ACERHU_03850) ruvX 797226..797642 (-) 417 WP_001014571.1 Holliday junction resolvase RuvX -
  ACERHU_RS03855 (ACERHU_03855) - 797642..798205 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  ACERHU_RS03860 (ACERHU_03860) gshB 798421..799368 (-) 948 WP_000593248.1 glutathione synthase -
  ACERHU_RS03865 (ACERHU_03865) rsmE 799388..800119 (-) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACERHU_RS03870 (ACERHU_03870) endA 800196..800903 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36149.35 Da        Isoelectric Point: 7.5116

>NTDB_id=1048784 ACERHU_RS03840 WP_001619985.1 795381..796361(+) (pilT) [Salmonella enterica strain SA136]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRAHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1048784 ACERHU_RS03840 WP_001619985.1 795381..796361(+) (pilT) [Salmonella enterica strain SA136]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAGCGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGTGCTACTGGCTGCGGAAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACACCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

46.395

97.853

0.454
  pilT Pseudomonas stutzeri DSM 10701

46.203

96.933

0.448
  pilT Pseudomonas aeruginosa PAK

46.203

96.933

0.448
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.858

100

0.374

Multiple sequence alignment