Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACCB35_RS15660 Genome accession   NZ_CP168654
Coordinates   3082915..3083952 (-) Length   345 a.a.
NCBI ID   WP_374054415.1    Uniprot ID   -
Organism   Rossellomorea sp. FM04394     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3077915..3088952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCB35_RS15630 - 3078092..3078850 (-) 759 WP_374054409.1 hypothetical protein -
  ACCB35_RS15635 - 3078847..3079464 (-) 618 WP_374054410.1 hypothetical protein -
  ACCB35_RS15640 pilM 3079448..3080419 (-) 972 WP_374054411.1 type IV pilus biogenesis protein PilM -
  ACCB35_RS15645 - 3080441..3081184 (-) 744 WP_374054412.1 A24 family peptidase -
  ACCB35_RS15650 - 3081185..3081583 (-) 399 WP_374054413.1 type II secretion system protein -
  ACCB35_RS15655 - 3081701..3082912 (-) 1212 WP_374054414.1 type II secretion system F family protein -
  ACCB35_RS15660 pilT 3082915..3083952 (-) 1038 WP_374054415.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCB35_RS15665 - 3083967..3085625 (-) 1659 WP_374054416.1 GspE/PulE family protein -
  ACCB35_RS15670 - 3085651..3086706 (-) 1056 WP_374054417.1 hypothetical protein -
  ACCB35_RS15675 - 3086971..3088683 (-) 1713 WP_374054418.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38552.41 Da        Isoelectric Point: 6.9818

>NTDB_id=1044784 ACCB35_RS15660 WP_374054415.1 3082915..3083952(-) (pilT) [Rossellomorea sp. FM04394]
MKERIDHLLRAAFELKASDIHLTVESAPVFRIHGDLKRYGQDVLKPEDTEQMAKAIVPEHLWTEFKERGEMDFSYALPGV
SRFRVNAYFQRSCISIALRVVPRNIPTLEELKLPPVLMKIAEKHQGLVLVTGPTGSGKSTTLASMIHYMNQTMRKHIITL
EDPIEYLHSHGSSIIDQREVGFDTKSFAYGLRSALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAVSTIE
RIIDVFPAEQQSQVRVQLASVLQGVIAQRLLPTVDKNGRIAVSEILLNNSAVANLIRSEKIHQIPSVMQTSKAQGMELFE
SNVSRYLHAGMISIEVASPFLQENR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1044784 ACCB35_RS15660 WP_374054415.1 3082915..3083952(-) (pilT) [Rossellomorea sp. FM04394]
TTGAAGGAACGGATAGATCATCTATTACGGGCGGCATTTGAATTAAAGGCCTCTGATATTCATTTAACAGTAGAATCGGC
ACCGGTTTTCCGCATTCATGGAGACTTGAAGAGGTATGGGCAGGATGTGCTGAAGCCGGAAGATACAGAGCAAATGGCTA
AAGCCATTGTTCCCGAGCATTTATGGACGGAATTCAAAGAGCGGGGGGAAATGGACTTTTCGTATGCACTCCCAGGTGTC
TCGCGGTTCCGTGTGAATGCCTATTTTCAACGATCTTGTATTTCCATTGCCTTACGGGTTGTGCCTAGGAATATTCCGAC
CCTGGAAGAACTGAAGCTCCCGCCTGTTTTAATGAAAATAGCAGAAAAGCATCAAGGGTTGGTGTTAGTGACCGGGCCGA
CCGGAAGCGGGAAATCGACGACCCTCGCTTCGATGATTCATTATATGAACCAGACGATGAGGAAGCATATCATAACACTT
GAGGATCCGATAGAGTACTTACATTCGCACGGTTCTTCCATCATTGATCAACGGGAAGTCGGGTTTGATACAAAGTCCTT
TGCATACGGATTGCGGAGTGCATTGAGGCAGGACCCCGATGTCATTCTGGTAGGGGAAATGCGTGACCTGGAAACCATTC
AAACAGCCATCACAGCAGCCGAGACCGGTCATTTAGTCCTGGGGACCCTGCATACGTCCAGTGCCGTTTCTACAATCGAG
CGGATCATAGATGTATTCCCGGCCGAACAGCAATCCCAGGTCCGGGTTCAGCTTGCATCGGTTCTGCAGGGAGTGATTGC
TCAGCGCCTCCTGCCTACGGTTGATAAAAATGGAAGGATTGCTGTCTCGGAAATCCTTTTGAATAATTCAGCCGTTGCCA
ATCTTATCCGGTCTGAAAAGATTCATCAAATTCCCTCCGTCATGCAAACCTCTAAAGCGCAGGGAATGGAGCTCTTTGAA
TCGAATGTCAGCAGATATCTGCACGCCGGTATGATTTCAATTGAAGTGGCATCTCCATTTCTTCAGGAGAACCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.599

96.812

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.846

90.435

0.487
  pilT Neisseria gonorrhoeae MS11

50

96.812

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

95.072

0.478
  pilT Vibrio cholerae strain A1552

50.305

95.072

0.478
  pilT Pseudomonas stutzeri DSM 10701

47.447

96.522

0.458
  pilT Legionella pneumophila strain Lp02

47.147

96.522

0.455
  pilT Legionella pneumophila strain ERS1305867

47.147

96.522

0.455
  pilT Pseudomonas aeruginosa PAK

46.246

96.522

0.446
  pilT Acinetobacter baylyi ADP1

45.646

96.522

0.441
  pilT Acinetobacter baumannii D1279779

46.341

95.072

0.441
  pilT Acinetobacter baumannii strain A118

46.341

95.072

0.441
  pilT Acinetobacter nosocomialis M2

46.341

95.072

0.441
  pilU Pseudomonas stutzeri DSM 10701

42.643

96.522

0.412
  pilU Vibrio cholerae strain A1552

44.262

88.406

0.391
  pilU Acinetobacter baylyi ADP1

40.063

91.884

0.368

Multiple sequence alignment