Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEPWQ_RS18875 Genome accession   NZ_CP168388
Coordinates   3957901..3958878 (-) Length   325 a.a.
NCBI ID   WP_338534081.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain LN01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952901..3963878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPWQ_RS18845 (ACEPWQ_18845) endA 3953489..3954196 (+) 708 WP_338534079.1 deoxyribonuclease I -
  ACEPWQ_RS18850 (ACEPWQ_18850) rsmE 3954308..3955039 (+) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEPWQ_RS18855 (ACEPWQ_18855) gshB 3955059..3956006 (+) 948 WP_032613926.1 glutathione synthase -
  ACEPWQ_RS18860 (ACEPWQ_18860) - 3956098..3956658 (+) 561 WP_032613928.1 YqgE/AlgH family protein -
  ACEPWQ_RS18865 (ACEPWQ_18865) ruvX 3956658..3957074 (+) 417 WP_130588229.1 Holliday junction resolvase RuvX -
  ACEPWQ_RS18870 (ACEPWQ_18870) - 3957040..3957801 (-) 762 WP_338534080.1 IclR family transcriptional regulator -
  ACEPWQ_RS18875 (ACEPWQ_18875) pilT 3957901..3958878 (-) 978 WP_338534081.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPWQ_RS18880 (ACEPWQ_18880) - 3958896..3959597 (+) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPWQ_RS18885 (ACEPWQ_18885) - 3959619..3960185 (+) 567 WP_032613937.1 YggT family protein -
  ACEPWQ_RS18890 (ACEPWQ_18890) yggU 3960182..3960478 (+) 297 WP_032613939.1 DUF167 family protein YggU -
  ACEPWQ_RS18895 (ACEPWQ_18895) - 3960482..3961075 (+) 594 WP_059307228.1 XTP/dITP diphosphatase -
  ACEPWQ_RS18900 (ACEPWQ_18900) hemW 3961068..3962210 (+) 1143 WP_373407754.1 radical SAM family heme chaperone HemW -
  ACEPWQ_RS18905 (ACEPWQ_18905) - 3962274..3962636 (+) 363 WP_338534082.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35703.88 Da        Isoelectric Point: 6.7692

>NTDB_id=1043911 ACEPWQ_RS18875 WP_338534081.1 3957901..3958878(-) (pilT) [Leclercia adecarboxylata strain LN01]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVMLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVLQTGLQTGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=1043911 ACEPWQ_RS18875 WP_338534081.1 3957901..3958878(-) (pilT) [Leclercia adecarboxylata strain LN01]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGACCGGATGTGGTGATGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGTGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTCAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
AGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACTGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACTCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAGCTGGCCGGCAGTTTGCAGGCGGTGCTGGCACAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTCATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGCTCCAGACCGGGCTGCAGACAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.489
  pilT Vibrio cholerae strain A1552

48.624

100

0.489
  pilT Neisseria meningitidis 8013

47.256

100

0.477
  pilT Legionella pneumophila strain Lp02

47.826

99.077

0.474
  pilT Legionella pneumophila strain ERS1305867

47.826

99.077

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.265

97.538

0.471
  pilT Acinetobacter baylyi ADP1

46.789

100

0.471
  pilT Neisseria gonorrhoeae MS11

47.5

98.462

0.468
  pilT Pseudomonas aeruginosa PAK

47.634

97.538

0.465
  pilT Acinetobacter baumannii strain A118

47.335

98.154

0.465
  pilT Acinetobacter baumannii D1279779

47.335

98.154

0.465
  pilT Acinetobacter nosocomialis M2

47.335

98.154

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

36.728

99.692

0.366

Multiple sequence alignment