Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEN2U_RS04855 Genome accession   NZ_CP168242
Coordinates   859994..861037 (+) Length   347 a.a.
NCBI ID   WP_003263111.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain 095-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 854994..866037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2U_RS04825 (ACEN2U_04825) - 856158..856400 (+) 243 WP_003263116.1 hypothetical protein -
  ACEN2U_RS04830 (ACEN2U_04830) - 856559..857059 (+) 501 WP_003271865.1 Dps family protein -
  ACEN2U_RS04835 (ACEN2U_04835) ubiA 857133..858008 (+) 876 WP_014616181.1 4-hydroxybenzoate octaprenyltransferase -
  ACEN2U_RS04840 (ACEN2U_04840) - 858005..858283 (-) 279 WP_042548948.1 hypothetical protein -
  ACEN2U_RS04845 (ACEN2U_04845) proC 858292..859116 (-) 825 WP_014616182.1 pyrroline-5-carboxylate reductase -
  ACEN2U_RS04850 (ACEN2U_04850) - 859150..859872 (-) 723 WP_014616183.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN2U_RS04855 (ACEN2U_04855) pilT 859994..861037 (+) 1044 WP_003263111.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN2U_RS04860 (ACEN2U_04860) pilU 861087..862226 (+) 1140 WP_003263110.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN2U_RS04865 (ACEN2U_04865) - 862422..862862 (+) 441 WP_014616184.1 type IV pilin protein -
  ACEN2U_RS04870 (ACEN2U_04870) - 862866..863354 (+) 489 WP_014616185.1 GspH/FimT family pseudopilin -
  ACEN2U_RS04875 (ACEN2U_04875) pilV 863351..863941 (+) 591 WP_039547379.1 type IV pilus modification protein PilV -
  ACEN2U_RS04880 (ACEN2U_04880) - 863938..864966 (+) 1029 WP_014616187.1 PilW family protein -
  ACEN2U_RS04885 (ACEN2U_04885) - 864970..865506 (+) 537 WP_014616188.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38166.80 Da        Isoelectric Point: 6.8705

>NTDB_id=1043411 ACEN2U_RS04855 WP_003263111.1 859994..861037(+) (pilT) [Ralstonia solanacearum strain 095-3]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFSDLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1043411 ACEN2U_RS04855 WP_003263111.1 859994..861037(+) (pilT) [Ralstonia solanacearum strain 095-3]
ATGGACATCGCGCAGCTTCTGGCCTTTGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCACCGAT
GATCCGGATCCATGGCGACATGCGTCGTATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCGGACTTCTCGTTCGAGATTCCCGGGTTGTCGCGT
TTCCGGGTCAACGCCTTTAACCAGAACCGTGGCGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCCGCCGTCTTCTCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACCACCGCAACGAAAGCGACCTGGGCCACATCCTCACGGTGGAGGAT
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGTGAGCTGGGGCCGCATACGCACTCGTTTGCCAA
TGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGGGACCTGGAAACCATCCGCCTGG
CACTGACGGCGGCCGAAACCGGCCACTTGGTGTTTGGTACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGCTGTCCGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATTTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAATGC
CTGGCGGAGCTCATCAAGCGCTCGGCAATCAACTACGCCGACGCACGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.118

0.646
  pilT Vibrio cholerae strain A1552

66.469

97.118

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.867

99.712

0.507
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment