Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABQY66_RS08485 Genome accession   NZ_CP168199
Coordinates   1968910..1969947 (+) Length   345 a.a.
NCBI ID   WP_022558867.1    Uniprot ID   A0AB34Q5X8
Organism   Xanthomonas citri strain WHRI 6104     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1963910..1974947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQY66_RS08455 (ABQY66_008460) - 1964177..1964719 (-) 543 WP_022558863.1 hypothetical protein -
  ABQY66_RS08460 (ABQY66_008465) - 1964776..1965117 (-) 342 WP_003489901.1 DUF3861 family protein -
  ABQY66_RS08465 (ABQY66_008470) - 1965175..1965816 (-) 642 WP_007966167.1 M23 family metallopeptidase -
  ABQY66_RS08470 (ABQY66_008475) - 1965919..1966344 (-) 426 WP_008571830.1 HU family DNA-binding protein -
  ABQY66_RS08475 (ABQY66_008480) proC 1966909..1967763 (-) 855 WP_022558865.1 pyrroline-5-carboxylate reductase -
  ABQY66_RS08480 (ABQY66_008485) - 1967807..1968499 (-) 693 WP_022558866.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABQY66_RS08485 (ABQY66_008490) pilT 1968910..1969947 (+) 1038 WP_022558867.1 type IV pilus twitching motility protein PilT Machinery gene
  ABQY66_RS08490 (ABQY66_008495) pilU 1970060..1971190 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABQY66_RS08495 (ABQY66_008500) - 1971692..1972366 (+) 675 WP_022558869.1 YitT family protein -
  ABQY66_RS08500 (ABQY66_008505) - 1972541..1973467 (-) 927 WP_173644273.1 DUF72 domain-containing protein -
  ABQY66_RS08505 (ABQY66_008510) - 1973475..1974047 (-) 573 WP_022558871.1 DNA-3-methyladenine glycosylase I -
  ABQY66_RS08510 (ABQY66_008515) - 1974315..1974881 (+) 567 WP_003489930.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38624.25 Da        Isoelectric Point: 6.5774

>NTDB_id=1043047 ABQY66_RS08485 WP_022558867.1 1968910..1969947(+) (pilT) [Xanthomonas citri strain WHRI 6104]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAHEYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1043047 ABQY66_RS08485 WP_022558867.1 1968910..1969947(+) (pilT) [Xanthomonas citri strain WHRI 6104]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCTCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTTCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTTGGCTGCCCGCCGATCTTCCGTCAGCTGATCGATCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGTCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGG
CGCTCACCGCTGCGGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATTTCGCAGGC
GCTGTTGAAGAAGGTCGGCGGCGGCCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCCCACGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment