Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACDW34_RS03855 Genome accession   NZ_CP167896
Coordinates   810149..811267 (-) Length   372 a.a.
NCBI ID   WP_130074022.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain p38     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 805149..816267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW34_RS03825 (ACDW34_03825) - 805811..806659 (+) 849 WP_174493242.1 class II glutamine amidotransferase -
  ACDW34_RS03830 (ACDW34_03830) - 807015..807470 (+) 456 WP_130074027.1 bacteriohemerythrin -
  ACDW34_RS03835 (ACDW34_03835) - 807625..808704 (+) 1080 WP_174493241.1 hypothetical protein -
  ACDW34_RS03840 (ACDW34_03840) - 808704..809042 (+) 339 WP_372404041.1 RnfH family protein -
  ACDW34_RS03845 (ACDW34_03845) - 809127..809525 (-) 399 WP_130074024.1 outer membrane protein assembly factor BamE -
  ACDW34_RS03850 (ACDW34_03850) fur 809638..810075 (+) 438 WP_130074023.1 ferric iron uptake transcriptional regulator -
  ACDW34_RS03855 (ACDW34_03855) pilU 810149..811267 (-) 1119 WP_130074022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDW34_RS03860 (ACDW34_03860) pilT 811293..812330 (-) 1038 WP_130074021.1 type IV pilus twitching motility protein PilT Machinery gene
  ACDW34_RS03865 (ACDW34_03865) - 812452..813141 (+) 690 WP_130074020.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACDW34_RS03870 (ACDW34_03870) - 813299..814093 (+) 795 WP_372404042.1 thioesterase family protein -
  ACDW34_RS03875 (ACDW34_03875) - 814234..814986 (+) 753 WP_372404043.1 hypothetical protein -
  ACDW34_RS03880 (ACDW34_03880) - 815159..815923 (+) 765 WP_372405064.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41756.01 Da        Isoelectric Point: 6.3495

>NTDB_id=1040162 ACDW34_RS03855 WP_130074022.1 810149..811267(-) (pilU) [Acinetobacter piscicola strain p38]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLTGELVGQIIHSIMSDKQRKEFAETRECNFAIVNKDKTA
RFRVSAFQQRDQPGMILRRIETTIPTMEDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASLIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANNKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1040162 ACDW34_RS03855 WP_130074022.1 810149..811267(-) (pilU) [Acinetobacter piscicola strain p38]
ATGGATTTTAATGATTTGCTTAATATGATGATTGAGCAAAAGGCATCGGACTTGTTTATTACTGCCGATGTTGAACCATC
AATGAAGATTAACGGTCAAATTGTTCCTGTGGTCAAAACTAAATTGACTGGAGAATTGGTCGGGCAAATTATTCACTCAA
TCATGAGTGATAAACAACGTAAAGAATTTGCCGAAACGCGTGAGTGCAACTTTGCCATTGTCAACAAAGATAAAACTGCA
CGTTTTCGTGTCAGTGCATTTCAGCAACGTGATCAACCTGGGATGATTTTACGTCGAATCGAAACCACGATTCCAACCAT
GGAAGATTTAAAGCTACCACCAATCTTAAAAGATCTCGCCATGACCAAGCGTGGCATTATCATTTTTGTAGGAGCAACAG
GTACAGGTAAATCGACCTCTTTGGCATCATTGATCGGTTACCGAAATCAAAACTCTAAAGGTCATATTATTACCATCGAA
GACCCAATCGAGTTCGTACATGAACATGCAGGCTGTATTATCACCCAACGTGAAGTAGGGATTGATACAGACTCTTTTGA
AGTCGCACTAAAAAATACTTTACGTCAAGCACCTGACGTCATTTTGATTGGTGAGATTCGTTCTCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTCTTTGCAACCCTGCATGCCAATAACGCCAACCAAGCCATCGATCGT
ATTATTCACTTCTTTGAATCAGACCGTCATAGTCAATTGTTTATGGACTTGTCCTTAAACCTCAAAGCCATGATTGCGCA
GCAACTCATCCCAACCCCAGATGGCAATTCACGCCGTGCTGCCATTGAGATTCTCATTAACTCGCCATTGATTTCAGATT
TGATCCGTAAAGGTGATGTACATGAAATCAAAGACTTAATGAAACGCTCACGTGAATTGGGTATGCAAACCTTTGACCAA
GCTTTATTTGATCTTTATAAAGCAGGTCAAATTACCTATAAAGATGCGCTAAAACATGCAGACTCACCAAATGACCTACG
TCTACAGATTAAGCTTGATGAAGAAGGTGGCGCACAGTTATTGAATGCCAACAACAAACTAACCTTTGACGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.766
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

55.616

98.118

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

93.011

0.392
  pilT Legionella pneumophila strain Lp02

42.308

90.86

0.384
  pilT Legionella pneumophila strain ERS1305867

42.308

90.86

0.384
  pilT Acinetobacter nosocomialis M2

40.816

92.204

0.376
  pilT Acinetobacter baumannii D1279779

40.816

92.204

0.376
  pilT Acinetobacter baumannii strain A118

40.816

92.204

0.376
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Acinetobacter baylyi ADP1

40.233

92.204

0.371
  pilT Pseudomonas stutzeri DSM 10701

39.884

93.011

0.371
  pilT Neisseria meningitidis 8013

39.595

93.011

0.368
  pilT Neisseria gonorrhoeae MS11

39.595

93.011

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.088

88.978

0.366
  pilT Vibrio cholerae strain A1552

41.088

88.978

0.366

Multiple sequence alignment