Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACDH69_RS14280 Genome accession   NZ_CP167866
Coordinates   3293486..3294511 (-) Length   341 a.a.
NCBI ID   WP_167763933.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:581     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3288486..3299511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH69_RS14255 (ACDH69_14255) - 3288558..3289124 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  ACDH69_RS14260 (ACDH69_14260) - 3289401..3289973 (+) 573 WP_008571824.1 DNA-3-methyladenine glycosylase I -
  ACDH69_RS14265 (ACDH69_14265) - 3289981..3290907 (+) 927 WP_031423562.1 DUF72 domain-containing protein -
  ACDH69_RS14270 (ACDH69_14270) - 3291081..3291755 (-) 675 WP_016851096.1 YitT family protein -
  ACDH69_RS14275 (ACDH69_14275) pilU 3292243..3293373 (-) 1131 WP_008571827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDH69_RS14280 (ACDH69_14280) pilT 3293486..3294511 (-) 1026 WP_167763933.1 type IV pilus twitching motility protein PilT Machinery gene
  ACDH69_RS14285 (ACDH69_14285) - 3294929..3295621 (+) 693 WP_008571828.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACDH69_RS14290 (ACDH69_14290) proC 3295665..3296519 (+) 855 WP_014090667.1 pyrroline-5-carboxylate reductase -
  ACDH69_RS14295 (ACDH69_14295) - 3297079..3297504 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  ACDH69_RS14300 (ACDH69_14300) - 3297609..3298250 (+) 642 WP_014090668.1 M23 family metallopeptidase -
  ACDH69_RS14305 (ACDH69_14305) - 3298308..3298649 (+) 342 WP_003489901.1 DUF3861 family protein -
  ACDH69_RS14310 (ACDH69_14310) - 3298706..3299248 (+) 543 WP_338334170.1 hypothetical protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38215.79 Da        Isoelectric Point: 6.5051

>NTDB_id=1040024 ACDH69_RS14280 WP_167763933.1 3293486..3294511(-) (pilT) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:581]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRIIDVF
PAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHLQDLV
KRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1040024 ACDH69_RS14280 WP_167763933.1 3293486..3294511(-) (pilT) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:581]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCGTTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTTCTCGAGGTCGACTTTTCGTTCGAAATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCAGGCATGATCGACTACATCAACAAGAATGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGTTTCAACGA
AGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGGCGCTTACCGCCG
CGGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATCATCGACGTGTTC
CCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATCTCGCAGGCACTGCTGAAGAA
GGTCGGCGGCGGCCGCACGGCGGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCCGCGAGGACAAGG
TGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGATCAGCATCTGCAGGACCTGGTC
AAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

100

0.795
  pilT Acinetobacter baumannii D1279779

78.779

100

0.795
  pilT Acinetobacter baumannii strain A118

78.779

100

0.795
  pilT Pseudomonas stutzeri DSM 10701

78.198

100

0.789
  pilT Acinetobacter baylyi ADP1

77.616

100

0.783
  pilT Pseudomonas aeruginosa PAK

77.876

99.413

0.774
  pilT Legionella pneumophila strain Lp02

74.128

100

0.748
  pilT Legionella pneumophila strain ERS1305867

74.128

100

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

96.481

0.674
  pilT Vibrio cholerae strain A1552

69.909

96.481

0.674
  pilT Neisseria gonorrhoeae MS11

64.809

100

0.648
  pilT Neisseria meningitidis 8013

64.809

100

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.413

99.413

0.402
  pilU Acinetobacter baylyi ADP1

40

99.707

0.399
  pilU Vibrio cholerae strain A1552

40.299

98.24

0.396

Multiple sequence alignment