Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACCP93_RS13155 Genome accession   NZ_CP167216
Coordinates   3024815..3025852 (-) Length   345 a.a.
NCBI ID   WP_218559506.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. fici strain NCPPB 2372     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3019815..3030852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP93_RS13130 (ACCP93_13130) - 3020393..3020965 (+) 573 WP_014090665.1 DNA-3-methyladenine glycosylase I -
  ACCP93_RS13135 (ACCP93_13135) - 3020973..3021899 (+) 927 WP_218559508.1 DUF72 domain-containing protein -
  ACCP93_RS13140 (ACCP93_13140) - 3022191..3022442 (+) 252 WP_162010796.1 hypothetical protein -
  ACCP93_RS13145 (ACCP93_13145) - 3022478..3023482 (+) 1005 WP_218559507.1 pectate lyase -
  ACCP93_RS13150 (ACCP93_13150) pilU 3023572..3024702 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCP93_RS13155 (ACCP93_13155) pilT 3024815..3025852 (-) 1038 WP_218559506.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCP93_RS13160 (ACCP93_13160) - 3026264..3026956 (+) 693 WP_218559505.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCP93_RS13165 (ACCP93_13165) proC 3027000..3027854 (+) 855 WP_011348110.1 pyrroline-5-carboxylate reductase -
  ACCP93_RS13170 (ACCP93_13170) - 3028414..3028839 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  ACCP93_RS13175 (ACCP93_13175) - 3028946..3029587 (+) 642 WP_218559504.1 M23 family metallopeptidase -
  ACCP93_RS13180 (ACCP93_13180) - 3029645..3029986 (+) 342 WP_017158997.1 DUF3861 family protein -
  ACCP93_RS13185 (ACCP93_13185) - 3030043..3030585 (+) 543 WP_343230355.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38642.31 Da        Isoelectric Point: 6.8355

>NTDB_id=1038906 ACCP93_RS13155 WP_218559506.1 3024815..3025852(-) (pilT) [Xanthomonas campestris pv. fici strain NCPPB 2372]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLENLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1038906 ACCP93_RS13155 WP_218559506.1 3024815..3025852(-) (pilT) [Xanthomonas campestris pv. fici strain NCPPB 2372]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGTTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTTTCGTTCGAAATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AAACCTTGGCTGCCCGCCGATCTTCCGTCAGCTGATCGATCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAATGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGG
CGCTCACCGCTGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCTGTGATTTCGCAGGC
GCTGTTGAAGAAGGTCGGCGGCGGCCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment