Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACBZ91_RS10375 Genome accession   NZ_CP167179
Coordinates   2231659..2232699 (-) Length   346 a.a.
NCBI ID   WP_014233032.1    Uniprot ID   -
Organism   Vibrio natriegens strain JSH01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2226659..2237699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACBZ91_RS10350 (ACBZ91_10350) rsmE 2227352..2228083 (+) 732 WP_118119390.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACBZ91_RS10355 (ACBZ91_10355) gshB 2228098..2229047 (+) 950 Protein_2015 glutathione synthase -
  ACBZ91_RS10360 (ACBZ91_10360) - 2229427..2229990 (+) 564 WP_014233029.1 YqgE/AlgH family protein -
  ACBZ91_RS10365 (ACBZ91_10365) ruvX 2230032..2230457 (+) 426 WP_372377336.1 Holliday junction resolvase RuvX -
  ACBZ91_RS10370 (ACBZ91_10370) pilU 2230523..2231629 (-) 1107 WP_014233031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACBZ91_RS10375 (ACBZ91_10375) pilT 2231659..2232699 (-) 1041 WP_014233032.1 type IV pilus twitching motility protein PilT Machinery gene
  ACBZ91_RS10380 (ACBZ91_10380) - 2232728..2233441 (+) 714 WP_372377338.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACBZ91_RS10385 (ACBZ91_10385) proC 2233564..2234382 (+) 819 WP_329348872.1 pyrroline-5-carboxylate reductase -
  ACBZ91_RS10390 (ACBZ91_10390) - 2234435..2234992 (+) 558 WP_014233035.1 YggT family protein -
  ACBZ91_RS10395 (ACBZ91_10395) yggU 2234992..2235282 (+) 291 WP_014233036.1 DUF167 family protein YggU -
  ACBZ91_RS10400 (ACBZ91_10400) - 2235357..2235788 (+) 432 WP_020334002.1 DUF4426 domain-containing protein -
  ACBZ91_RS10405 (ACBZ91_10405) - 2235922..2236524 (+) 603 WP_372377339.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38247.54 Da        Isoelectric Point: 6.3867

>NTDB_id=1038690 ACBZ91_RS10375 WP_014233032.1 2231659..2232699(-) (pilT) [Vibrio natriegens strain JSH01]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELQNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLDQLEAPEIFSKIASGQKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLSSVIAQKLLKRNGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLLAQGIVSHEEADSKVGLDVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1038690 ACBZ91_RS10375 WP_014233032.1 2231659..2232699(-) (pilT) [Vibrio natriegens strain JSH01]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCAGGTGTACCACCTAT
GGTCAGAATTGATGGCGATGTGAGAAAGCTCGGGATTCCAGCGTTTACACACCAAGAGGTTCATCGCTTGGTATTCGAAA
TCATGAATGACGCTCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTTTCTTTTGAACTACAGAACGTTGGTCGC
TTTCGTGTCAACGCATTTAACCAGTCCCGCGGCTGCGCGGCGGTATTTCGTACCATTCCAAGCAGCATACCAACGCTTGA
TCAACTCGAAGCTCCAGAAATCTTCAGCAAGATTGCCAGCGGGCAAAAAGGCTTGGTTCTGGTAACCGGACCAACCGGCT
CTGGTAAATCGACGACTCTAGCTGCCATTGTCGACTACATCAATCGTAATCACAATAAGCACATTCTCACAATTGAAGAT
CCAATTGAATTTGTTCATACCAACAACAAATGTTTGATCAACCAGCGCGAAGTTCACCGTGACACTCATAGCTTCCAAAA
CGCCTTGCGCAGTGCGTTGCGTGAAGACCCGGATGTCATCTTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGCCTAG
CATTGACCGCCGCAGAAACCGGTCATTTGGTTTTTGGAACGCTACACACCAGCTCAGCCGCAAAAACCATCGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCGATGTTGTCGGAATCGTTGAGTTCAGTCATTGCCCAGAA
GTTACTAAAGCGTAATGGTGGTGGCCGAGTCGCCTGCCATGAAATCATGATGGCGACACCAGCAATTCGTAACTTGATTC
GCGAAGATAAAGTCGCACAGATGTACTCGATAATTCAAACTGGTGCGGCACACGGTATGCAGACAATGGAGCAAAATGCT
CGTCAGCTATTAGCACAAGGTATTGTTTCTCACGAAGAGGCCGACAGCAAAGTTGGGCTTGATGTACAGCAGTTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

71.856

96.532

0.694
  pilT Acinetobacter baumannii strain A118

71.856

96.532

0.694
  pilT Acinetobacter nosocomialis M2

71.557

96.532

0.691
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.399

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Legionella pneumophila strain Lp02

67.742

98.555

0.668
  pilT Legionella pneumophila strain ERS1305867

67.742

98.555

0.668
  pilT Neisseria meningitidis 8013

65.373

96.821

0.633
  pilT Neisseria gonorrhoeae MS11

65.075

96.821

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.991

100

0.491
  pilU Vibrio cholerae strain A1552

41.194

96.821

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.821

0.393
  pilU Acinetobacter baylyi ADP1

38.393

97.11

0.373

Multiple sequence alignment