Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACAW63_RS02870 Genome accession   NZ_CP166873
Coordinates   633732..634172 (-) Length   146 a.a.
NCBI ID   WP_371231555.1    Uniprot ID   -
Organism   Pseudomonas sp. QE6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 628732..639172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAW63_RS02850 (ACAW63_02850) - 629138..629677 (+) 540 WP_371231550.1 type 1 glutamine amidotransferase domain-containing protein -
  ACAW63_RS02855 (ACAW63_02855) - 629770..630966 (+) 1197 WP_371231552.1 class I SAM-dependent rRNA methyltransferase -
  ACAW63_RS02860 (ACAW63_02860) - 631140..631454 (-) 315 WP_371231553.1 hypothetical protein -
  ACAW63_RS02865 (ACAW63_02865) ilvD 631830..633668 (+) 1839 WP_371231554.1 dihydroxy-acid dehydratase -
  ACAW63_RS02870 (ACAW63_02870) pilA2 633732..634172 (-) 441 WP_371231555.1 pilin Machinery gene
  ACAW63_RS02875 (ACAW63_02875) - 634477..635868 (+) 1392 WP_371231557.1 nucleobase:cation symporter-2 family protein -
  ACAW63_RS02880 (ACAW63_02880) - 635918..636385 (+) 468 WP_371231558.1 metallophosphoesterase family protein -
  ACAW63_RS02885 (ACAW63_02885) - 636470..637843 (-) 1374 WP_371231560.1 GTPase/DUF3482 domain-containing protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14985.08 Da        Isoelectric Point: 5.0804

>NTDB_id=1035862 ACAW63_RS02870 WP_371231555.1 633732..634172(-) (pilA2) [Pseudomonas sp. QE6]
MSAIRTVQKGFTLIELMIVVAIIGILAAIAIPAYQDYLARSRVSEGLALAAAAKTGVTEYYASNGSWPTSNDMAGIDEAE
NIKGNSVTSVEVGAGGIVTITYNAKLGAAGSGKTVVLTPTNNGGSISWTCTDGDMPGKLRPAECRS

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1035862 ACAW63_RS02870 WP_371231555.1 633732..634172(-) (pilA2) [Pseudomonas sp. QE6]
ATGAGTGCGATTCGTACAGTACAAAAGGGTTTCACCCTTATTGAATTGATGATCGTTGTGGCGATCATTGGCATTCTGGC
GGCGATTGCTATTCCGGCTTACCAAGACTATCTGGCTAGGTCCCGGGTCAGTGAAGGCTTGGCGTTGGCTGCTGCAGCAA
AAACTGGCGTGACTGAGTATTATGCTTCTAATGGCTCTTGGCCAACAAGTAACGATATGGCCGGAATTGATGAGGCTGAG
AATATTAAAGGGAATAGCGTAACGTCAGTAGAGGTTGGTGCGGGTGGGATTGTTACAATTACCTACAATGCCAAGCTTGG
CGCAGCTGGTAGTGGTAAGACGGTTGTTCTAACTCCTACTAATAATGGTGGCTCTATTTCTTGGACTTGTACGGATGGTG
ATATGCCGGGCAAGTTGCGTCCTGCCGAGTGCCGTAGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)

Pilin (bacterial filament) (Pilin)

(39-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

53.237

95.205

0.507
  pilA2 Legionella pneumophila strain ERS1305867

52.518

95.205

0.5
  pilA Ralstonia pseudosolanacearum GMI1000

42.012

100

0.486
  comP Acinetobacter baylyi ADP1

44.667

100

0.459
  pilE Neisseria gonorrhoeae strain FA1090

50

90.411

0.452
  pilA/pilA1 Eikenella corrodens VA1

39.873

100

0.432
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.662

97.26

0.425
  pilE Neisseria gonorrhoeae MS11

47.656

87.671

0.418
  pilA Acinetobacter baumannii strain A118

42.657

97.945

0.418
  pilA Pseudomonas aeruginosa PAK

40.541

100

0.411
  pilA Vibrio cholerae C6706

39.333

100

0.404
  pilA Vibrio cholerae strain A1552

39.333

100

0.404
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.333

100

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.838

100

0.384
  pilA Vibrio campbellii strain DS40M4

37.241

99.315

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

42.742

84.932

0.363

Multiple sequence alignment