Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACA027_RS20585 Genome accession   NZ_CP166730
Coordinates   4647060..4648106 (+) Length   348 a.a.
NCBI ID   WP_370680047.1    Uniprot ID   -
Organism   Comamonas sp. GB3 AK4-5     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4642060..4653106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA027_RS20570 (ACA027_20570) - 4643786..4645384 (-) 1599 WP_370680044.1 AMP-binding protein -
  ACA027_RS20575 (ACA027_20575) - 4645549..4646313 (+) 765 WP_370680045.1 TetR/AcrR family transcriptional regulator -
  ACA027_RS20580 (ACA027_20580) - 4646298..4647017 (-) 720 WP_370680046.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACA027_RS20585 (ACA027_20585) pilT 4647060..4648106 (+) 1047 WP_370680047.1 type IV pilus twitching motility protein PilT Machinery gene
  ACA027_RS20590 (ACA027_20590) - 4648258..4649160 (+) 903 WP_370680048.1 NAD(P)-dependent oxidoreductase -
  ACA027_RS20595 (ACA027_20595) - 4649392..4650054 (-) 663 WP_370680049.1 BON domain-containing protein -
  ACA027_RS20600 (ACA027_20600) - 4650051..4650647 (-) 597 WP_370680050.1 SIS domain-containing protein -
  ACA027_RS20605 (ACA027_20605) - 4650723..4651121 (-) 399 WP_370680051.1 YraN family protein -
  ACA027_RS20610 (ACA027_20610) rsmI 4651121..4652038 (+) 918 WP_370680052.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 37830.57 Da        Isoelectric Point: 7.6593

>NTDB_id=1035059 ACA027_RS20585 WP_370680047.1 4647060..4648106(+) (pilT) [Comamonas sp. GB3 AK4-5]
MDITRLLAFGVQHQASDLHLSAGLPPMVRVHGDMRRIHAQALDHRTVLGMLHETMGDLQRKVFEASLEVDFAFEIPGLAR
FRVNAFQQSRGAAAVFRTIPSTVPTLEQLGSPPILAELALKPRGLVLVTGPTGSGKSTTLAAMLNHLNDHLHGHILTVED
PIEFVHASKKCLVNQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFATLHTASAAKTIDRI
IDVFPAGEKDMVRSMLSESLQAVVSQTLCKTREGTGRVAAHEIMLGSSAIRHLIRESKVAQMYSTIQTSQSLGMQTLDQS
LSDLLRRQCISAAEARSKARTPESFPGG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=1035059 ACA027_RS20585 WP_370680047.1 4647060..4648106(+) (pilT) [Comamonas sp. GB3 AK4-5]
GTGGACATCACCCGCTTGCTGGCCTTTGGCGTGCAGCACCAGGCCTCGGACCTGCATCTTTCGGCCGGGCTGCCTCCCAT
GGTCCGCGTGCACGGTGATATGCGCCGCATCCATGCCCAAGCGCTAGACCACAGAACCGTGCTCGGCATGCTCCATGAAA
CCATGGGCGATTTGCAGCGCAAGGTATTTGAAGCCTCGCTGGAGGTGGATTTTGCGTTCGAGATCCCGGGCCTGGCACGC
TTTCGGGTGAATGCATTCCAGCAAAGCCGGGGCGCTGCCGCGGTGTTCCGCACCATTCCCAGCACCGTGCCGACGCTGGA
GCAACTGGGCAGCCCCCCGATTCTTGCCGAGCTGGCCCTCAAGCCCCGTGGCCTGGTGTTGGTCACCGGCCCTACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGCTCAACCACCTCAACGACCACTTGCACGGCCATATCCTGACCGTGGAAGAC
CCCATCGAGTTTGTCCATGCCTCCAAGAAATGCCTGGTCAACCAGCGTGAGGTGGGGCCCATGACCCAGTCGTTTTCCGC
CGCACTGCGCTCGGCGCTGCGTGAAGACCCCGACGCCATCCTGGTGGGCGAGATGCGCGATCTGGAAACCATACGCCTGG
CCATGACGGCAGCCGAGACCGGCCACCTGGTCTTTGCCACGCTGCACACCGCCAGCGCAGCCAAGACCATAGACCGCATC
ATTGACGTCTTCCCCGCTGGCGAAAAAGACATGGTGCGCAGCATGCTGTCCGAATCGCTGCAGGCCGTGGTCTCGCAGAC
GCTGTGCAAAACCAGGGAAGGCACAGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCAGCAGTGCCATACGCCACCTGA
TCCGCGAGTCCAAGGTGGCGCAGATGTATTCCACCATCCAGACCAGCCAGAGCCTGGGCATGCAGACCCTGGACCAGAGC
CTGAGCGATCTGCTGCGCCGCCAGTGCATCAGCGCTGCAGAGGCCCGCAGCAAGGCCAGGACGCCTGAGAGCTTCCCCGG
CGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

70.145

99.138

0.695
  pilT Pseudomonas stutzeri DSM 10701

70.145

99.138

0.695
  pilT Pseudomonas aeruginosa PAK

69.855

99.138

0.693
  pilT Acinetobacter nosocomialis M2

68.986

99.138

0.684
  pilT Acinetobacter baumannii D1279779

68.986

99.138

0.684
  pilT Acinetobacter baumannii strain A118

68.986

99.138

0.684
  pilT Legionella pneumophila strain Lp02

66.957

99.138

0.664
  pilT Legionella pneumophila strain ERS1305867

66.957

99.138

0.664
  pilT Neisseria meningitidis 8013

64.058

99.138

0.635
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.089

97.126

0.632
  pilT Vibrio cholerae strain A1552

65.089

97.126

0.632
  pilT Neisseria gonorrhoeae MS11

63.768

99.138

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

99.138

0.483
  pilU Vibrio cholerae strain A1552

43.881

96.264

0.422
  pilU Acinetobacter baylyi ADP1

41.04

99.425

0.408
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.264

0.405

Multiple sequence alignment