Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8806_RS08875 Genome accession   NZ_CP166102
Coordinates   1908768..1909811 (-) Length   347 a.a.
NCBI ID   WP_369951858.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain NCPPB 3728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1903768..1914811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8806_RS08845 (AB8806_08840) - 1904306..1904842 (-) 537 WP_078222017.1 PilX N-terminal domain-containing pilus assembly protein -
  AB8806_RS08850 (AB8806_08845) - 1904846..1905868 (-) 1023 WP_013211550.1 PilW family protein -
  AB8806_RS08855 (AB8806_08850) pilV 1905865..1906455 (-) 591 WP_078222019.1 type IV pilus modification protein PilV -
  AB8806_RS08860 (AB8806_08855) - 1906452..1906940 (-) 489 WP_078222020.1 GspH/FimT family pseudopilin -
  AB8806_RS08865 (AB8806_08860) - 1906944..1907384 (-) 441 WP_078222021.1 type IV pilin protein -
  AB8806_RS08870 (AB8806_08865) pilU 1907579..1908715 (-) 1137 WP_078222022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8806_RS08875 (AB8806_08870) pilT 1908768..1909811 (-) 1044 WP_369951858.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8806_RS08880 (AB8806_08875) - 1909934..1910656 (+) 723 WP_075463463.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8806_RS08885 (AB8806_08880) proC 1910691..1911515 (+) 825 WP_013211543.1 pyrroline-5-carboxylate reductase -
  AB8806_RS08890 (AB8806_08885) - 1911528..1911806 (+) 279 WP_013211542.1 hypothetical protein -
  AB8806_RS08895 (AB8806_08890) ubiA 1911803..1912681 (-) 879 WP_078222023.1 4-hydroxybenzoate octaprenyltransferase -
  AB8806_RS08900 (AB8806_08895) - 1912755..1913255 (-) 501 WP_003271865.1 Dps family protein -
  AB8806_RS08905 (AB8806_08900) - 1913417..1913659 (-) 243 WP_013211540.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38108.72 Da        Isoelectric Point: 6.8705

>NTDB_id=1033437 AB8806_RS08875 WP_369951858.1 1908768..1909811(-) (pilT) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3728]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPALTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLPKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1033437 AB8806_RS08875 WP_369951858.1 1908768..1909811(-) (pilT) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3728]
ATGGACATCGCGCAGCTTCTGGCCTTCGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCCCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCAGCGCTCACGCACCAGGATGTTCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCCGACTTCTCTTTCGAGATCCCCGGGCTGTCCCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGCGGCGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCGCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACCGCAACGAAAGCGACCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGGCCACATACCCATTCGTTTGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CACTGACGGCGGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGTTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTGCCCAAGACGCGCGACGGCTCCGGCCGGGTCGCTGCGCACGAGATCATGATCTGCACGCCGGCCATCCGCCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTCATCAAGCGTTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAATCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

68.208

99.712

0.68
  pilT Neisseria meningitidis 8013

68.208

99.712

0.68
  pilT Legionella pneumophila strain Lp02

67.246

99.424

0.669
  pilT Legionella pneumophila strain ERS1305867

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment