Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8814_RS18915 Genome accession   NZ_CP166090
Coordinates   4445617..4446750 (+) Length   377 a.a.
NCBI ID   WP_205392514.1    Uniprot ID   -
Organism   Xanthomonas hydrangeae strain NCPPB 4692     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4440617..4451750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8814_RS18890 (AB8814_18885) - 4440827..4442014 (-) 1188 WP_205392511.1 cystathionine gamma-synthase -
  AB8814_RS18895 (AB8814_18890) - 4442104..4443474 (-) 1371 WP_205392512.1 pyridoxal-phosphate dependent enzyme -
  AB8814_RS18900 (AB8814_18895) - 4443693..4443911 (-) 219 WP_002814034.1 YdcH family protein -
  AB8814_RS18905 (AB8814_18900) - 4444248..4445138 (-) 891 WP_205392513.1 hypothetical protein -
  AB8814_RS18910 (AB8814_18905) - 4445135..4445518 (-) 384 WP_006449646.1 DUF4398 domain-containing protein -
  AB8814_RS18915 (AB8814_18910) pilU 4445617..4446750 (+) 1134 WP_205392514.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8814_RS18920 (AB8814_18915) maiA 4447103..4447765 (-) 663 WP_205392515.1 maleylacetoacetate isomerase -
  AB8814_RS18925 (AB8814_18920) - 4447821..4448879 (-) 1059 WP_205394932.1 fumarylacetoacetate hydrolase family protein -
  AB8814_RS18930 (AB8814_18925) - 4449188..4450576 (+) 1389 WP_006450465.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41755.05 Da        Isoelectric Point: 6.2915

>NTDB_id=1033328 AB8814_RS18915 WP_205392514.1 4445617..4446750(+) (pilU) [Xanthomonas hydrangeae strain NCPPB 4692]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAISLPDAGR
FRVNVFKQRGEAGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLLVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGQDGRRRPATEVLLNTPMIRDLLRRGKVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1033328 AB8814_RS18915 WP_205392514.1 4445617..4446750(+) (pilU) [Xanthomonas hydrangeae strain NCPPB 4692]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTTTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAACTCAACATGGCCATCTCGCTGCCCGACGCCGGACGC
TTCCGTGTAAACGTGTTCAAGCAGCGCGGCGAAGCAGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACGCCGCGCGGCCTGGTGCTGCTGGTCGGCTCCACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCATCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGACACCCACACCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CGATTGCGTTTGCCGAAACCGGTCACCTGTGTCTGGCGACGCTGCATTCCAACAATGCCGATCAGACCATCGAGCGCATT
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAACTTGCGTGCGGTGGTGTCGCAACG
CCTGGTCAAGGGCCAGGACGGACGCCGCCGCCCCGCCACCGAAGTCTTGCTCAACACCCCGATGATTCGCGACCTGCTGC
GACGCGGCAAGGTGCACGAGGTCAAGGCGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAATCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCCGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAACACGACCCGTATGCGGATTACGAAACGGCCGCAGCGCCCAAGATCAGCC
ACGGGTTTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.925

95.225

0.504
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.717

100

0.443
  pilT Pseudomonas aeruginosa PAK

43.195

89.655

0.387
  pilT Acinetobacter baumannii strain A118

41.716

89.655

0.374
  pilT Acinetobacter nosocomialis M2

41.716

89.655

0.374
  pilT Acinetobacter baumannii D1279779

41.716

89.655

0.374
  pilT Legionella pneumophila strain Lp02

41.716

89.655

0.374
  pilT Legionella pneumophila strain ERS1305867

41.716

89.655

0.374
  pilT Acinetobacter baylyi ADP1

41.176

90.186

0.371
  pilT Neisseria gonorrhoeae MS11

39.943

92.308

0.369
  pilT Neisseria meningitidis 8013

39.943

92.308

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.617

88.594

0.369
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361

Multiple sequence alignment