Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   AB8E26_RS12555 Genome accession   NZ_CP165963
Coordinates   2779293..2779727 (+) Length   144 a.a.
NCBI ID   WP_369970414.1    Uniprot ID   -
Organism   Stenotrophomonas rhizophila strain busan1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2773591..2781922 2779293..2779727 within 0


Gene organization within MGE regions


Location: 2773591..2781922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E26_RS12525 (AB8E26_12535) - 2773600..2774064 (+) 465 WP_328803786.1 hypothetical protein -
  AB8E26_RS12530 (AB8E26_12540) - 2774073..2774750 (+) 678 WP_017356179.1 response regulator transcription factor -
  AB8E26_RS12535 (AB8E26_12545) - 2774713..2776080 (+) 1368 WP_369968045.1 sensor histidine kinase -
  AB8E26_RS12540 (AB8E26_12550) coaE 2776177..2776794 (-) 618 WP_369968046.1 dephospho-CoA kinase -
  AB8E26_RS12545 (AB8E26_12555) - 2776805..2777668 (-) 864 WP_128095489.1 A24 family peptidase -
  AB8E26_RS12550 (AB8E26_12560) pilC 2777676..2778935 (-) 1260 WP_369968047.1 type II secretion system F family protein Machinery gene
  AB8E26_RS12555 (AB8E26_12565) pilA2 2779293..2779727 (+) 435 WP_369970414.1 pilin Machinery gene
  AB8E26_RS12560 (AB8E26_12570) - 2779798..2781009 (+) 1212 WP_369968048.1 isoprenylcysteine carboxylmethyltransferase family protein -
  AB8E26_RS12565 (AB8E26_12575) - 2780996..2781922 (+) 927 WP_369968049.1 serine hydrolase -

Sequence


Protein


Download         Length: 144 a.a.        Molecular weight: 14392.67 Da        Isoelectric Point: 8.4715

>NTDB_id=1032577 AB8E26_RS12555 WP_369970414.1 2779293..2779727(+) (pilA2) [Stenotrophomonas rhizophila strain busan1-1]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKVSEVVVMAAPAKLAVSETSSSLGGLATVTAANAGYTFPGATK
YVSGITVADGGIVTAASSVPGAAGNIVLTPKDVGNGQLTWTCSTTIDKKFVPAECRGAAAATGG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1032577 AB8E26_RS12555 WP_369970414.1 2779293..2779727(+) (pilA2) [Stenotrophomonas rhizophila strain busan1-1]
ATGAAGAAGCAGCAGGGCTTTACCCTTATCGAACTGATGATCGTTGTCGCGATCATCGCCATCCTGGCCGCCATCGCACT
GCCGGCTTACCAGGACTACACCACCCGCGCGAAGGTGTCGGAAGTGGTCGTGATGGCTGCGCCGGCCAAGCTGGCTGTTT
CGGAGACGTCTTCGTCGCTGGGCGGCCTGGCCACGGTGACCGCTGCCAACGCTGGTTACACCTTCCCGGGCGCCACCAAG
TACGTGTCGGGCATCACCGTTGCTGATGGCGGCATCGTCACTGCGGCTTCGAGCGTTCCCGGCGCCGCCGGCAACATCGT
GCTGACCCCGAAGGATGTCGGTAACGGTCAGCTGACCTGGACCTGCTCCACCACCATCGACAAGAAGTTCGTCCCGGCAG
AATGCCGTGGCGCTGCTGCTGCGACGGGCGGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.852

93.75

0.486

  comP Acinetobacter baylyi ADP1

46.622

100

0.479

  pilA2 Legionella pneumophila strain ERS1305867

51.111

93.75

0.479

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.595

100

0.444

  pilE Neisseria gonorrhoeae strain FA1090

38.182

100

0.438

  pilA Ralstonia pseudosolanacearum GMI1000

37.805

100

0.431

  pilE Neisseria gonorrhoeae MS11

37.037

100

0.417

  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.912

94.444

0.396

  pilA Glaesserella parasuis strain SC1401

37.013

100

0.396

  pilA Haemophilus influenzae Rd KW20

39.041

100

0.396

  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.389

  pilA Pseudomonas aeruginosa PAK

35.484

100

0.382

  pilA Haemophilus influenzae 86-028NP

37.671

100

0.382

  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.259

93.75

0.368


Multiple sequence alignment