Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4J74_RS20320 Genome accession   NZ_CP163502
Coordinates   4291791..4292813 (-) Length   340 a.a.
NCBI ID   WP_131165516.1    Uniprot ID   -
Organism   Serratia marcescens strain XZ-60S     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4286791..4297813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4J74_RS20290 (AB4J74_20290) - 4287604..4288116 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  AB4J74_RS20295 (AB4J74_20295) endA 4288218..4288913 (+) 696 WP_131165517.1 deoxyribonuclease I -
  AB4J74_RS20300 (AB4J74_20300) rsmE 4288983..4289714 (+) 732 WP_004937458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB4J74_RS20305 (AB4J74_20305) gshB 4289725..4290675 (+) 951 WP_033632096.1 glutathione synthase -
  AB4J74_RS20310 (AB4J74_20310) - 4290809..4291372 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  AB4J74_RS20315 (AB4J74_20315) ruvX 4291372..4291794 (+) 423 WP_103830044.1 Holliday junction resolvase RuvX -
  AB4J74_RS20320 (AB4J74_20320) pilT 4291791..4292813 (-) 1023 WP_131165516.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4J74_RS20325 (AB4J74_20325) - 4292834..4293541 (+) 708 WP_004937443.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4J74_RS20330 (AB4J74_20330) proC 4293561..4294382 (+) 822 WP_004937440.1 pyrroline-5-carboxylate reductase -
  AB4J74_RS20335 (AB4J74_20335) - 4294414..4294968 (+) 555 WP_004937436.1 YggT family protein -
  AB4J74_RS20340 (AB4J74_20340) yggU 4294965..4295255 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  AB4J74_RS20345 (AB4J74_20345) - 4295274..4295867 (+) 594 WP_131165515.1 XTP/dITP diphosphatase -
  AB4J74_RS20350 (AB4J74_20350) hemW 4295860..4297002 (+) 1143 WP_131165514.1 radical SAM family heme chaperone HemW -
  AB4J74_RS20355 (AB4J74_20355) - 4297039..4297287 (-) 249 WP_004937422.1 DUF2442 domain-containing protein -
  AB4J74_RS20360 (AB4J74_20360) - 4297271..4297513 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36429.75 Da        Isoelectric Point: 7.5736

>NTDB_id=1030118 AB4J74_RS20320 WP_131165516.1 4291791..4292813(-) (pilT) [Serratia marcescens strain XZ-60S]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAVPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1030118 AB4J74_RS20320 WP_131165516.1 4291791..4292813(-) (pilT) [Serratia marcescens strain XZ-60S]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAGTCGCCTTCGCT
TGCCGAGCTGGCGGTGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGTGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAGCGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

49.849

97.353

0.485
  pilT Neisseria gonorrhoeae MS11

49.547

97.353

0.482
  pilT Legionella pneumophila strain ERS1305867

48.795

97.647

0.476
  pilT Legionella pneumophila strain Lp02

48.795

97.647

0.476
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.182

97.059

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.539

100

0.456
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365

Multiple sequence alignment