Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB6N06_RS24680 Genome accession   NZ_CP163407
Coordinates   5191589..5192611 (-) Length   340 a.a.
NCBI ID   WP_182265366.1    Uniprot ID   -
Organism   Serratia marcescens strain 1.8.3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5186589..5197611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB6N06_RS24650 (AB6N06_24650) - 5187273..5187785 (+) 513 WP_033651326.1 SprT family zinc-dependent metalloprotease -
  AB6N06_RS24655 (AB6N06_24655) endA 5187887..5188582 (+) 696 WP_025304243.1 deoxyribonuclease I -
  AB6N06_RS24660 (AB6N06_24660) rsmE 5188767..5189498 (+) 732 WP_228206277.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB6N06_RS24665 (AB6N06_24665) gshB 5189509..5190459 (+) 951 WP_016930084.1 glutathione synthase -
  AB6N06_RS24670 (AB6N06_24670) - 5190607..5191170 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  AB6N06_RS24675 (AB6N06_24675) ruvX 5191170..5191592 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AB6N06_RS24680 (AB6N06_24680) pilT 5191589..5192611 (-) 1023 WP_182265366.1 type IV pilus twitching motility protein PilT Machinery gene
  AB6N06_RS24685 (AB6N06_24685) - 5192632..5193339 (+) 708 WP_042785369.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB6N06_RS24690 (AB6N06_24690) proC 5193359..5194180 (+) 822 WP_033651322.1 pyrroline-5-carboxylate reductase -
  AB6N06_RS24695 (AB6N06_24695) - 5194212..5194766 (+) 555 WP_042785370.1 YggT family protein -
  AB6N06_RS24700 (AB6N06_24700) yggU 5194763..5195053 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  AB6N06_RS24705 (AB6N06_24705) - 5195071..5195664 (+) 594 WP_182073824.1 XTP/dITP diphosphatase -
  AB6N06_RS24710 (AB6N06_24710) hemW 5195657..5196799 (+) 1143 WP_369605980.1 radical SAM family heme chaperone HemW -
  AB6N06_RS24715 (AB6N06_24715) - 5196837..5197271 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36363.60 Da        Isoelectric Point: 7.2888

>NTDB_id=1029548 AB6N06_RS24680 WP_182265366.1 5191589..5192611(-) (pilT) [Serratia marcescens strain 1.8.3]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1029548 AB6N06_RS24680 WP_182265366.1 5191589..5192611(-) (pilT) [Serratia marcescens strain 1.8.3]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCTGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCATCGCAGCCGACGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment