Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4O95_RS18945 Genome accession   NZ_CP163007
Coordinates   3960939..3961919 (-) Length   326 a.a.
NCBI ID   WP_196355071.1    Uniprot ID   -
Organism   Enterobacter asburiae strain W207     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3955939..3966919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4O95_RS18915 (AB4O95_18915) - 3956815..3957312 (+) 498 WP_033146604.1 SprT family zinc-dependent metalloprotease -
  AB4O95_RS18920 (AB4O95_18920) endA 3957407..3958114 (+) 708 WP_023333343.1 deoxyribonuclease I -
  AB4O95_RS18925 (AB4O95_18925) rsmE 3958166..3958897 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB4O95_RS18930 (AB4O95_18930) gshB 3958917..3959864 (+) 948 WP_033146605.1 glutathione synthase -
  AB4O95_RS18935 (AB4O95_18935) - 3959952..3960512 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  AB4O95_RS18940 (AB4O95_18940) ruvX 3960512..3960928 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  AB4O95_RS18945 (AB4O95_18945) pilT 3960939..3961919 (-) 981 WP_196355071.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4O95_RS18950 (AB4O95_18950) - 3961937..3962638 (+) 702 WP_087823220.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4O95_RS18955 (AB4O95_18955) - 3962660..3963226 (+) 567 WP_045888331.1 YggT family protein -
  AB4O95_RS18960 (AB4O95_18960) yggU 3963223..3963519 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  AB4O95_RS18965 (AB4O95_18965) - 3963523..3964116 (+) 594 WP_196355072.1 XTP/dITP diphosphatase -
  AB4O95_RS18970 (AB4O95_18970) hemW 3964109..3965245 (+) 1137 WP_196355073.1 radical SAM family heme chaperone HemW -
  AB4O95_RS18975 (AB4O95_18975) - 3965315..3965677 (+) 363 WP_071993605.1 endonuclease domain-containing protein -
  AB4O95_RS18980 (AB4O95_18980) - 3965739..3966455 (-) 717 WP_143346505.1 DUF2884 domain-containing protein -
  AB4O95_RS18985 (AB4O95_18985) - 3966512..3966838 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35534.57 Da        Isoelectric Point: 6.9108

>NTDB_id=1028432 AB4O95_RS18945 WP_196355071.1 3960939..3961919(-) (pilT) [Enterobacter asburiae strain W207]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGIWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1028432 AB4O95_RS18945 WP_196355071.1 3960939..3961919(-) (pilT) [Enterobacter asburiae strain W207]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTTTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

98.773

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment