Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   QUE46_RS14435 Genome accession   NZ_AP027922
Coordinates   3206940..3207377 (+) Length   145 a.a.
NCBI ID   WP_286245366.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. MM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3201940..3212377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE46_RS14415 (PspMM1_28100) pdhR 3202635..3203384 (-) 750 WP_089348719.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QUE46_RS14420 (PspMM1_28110) ampE 3203987..3204838 (-) 852 WP_286245363.1 beta-lactamase regulator AmpE -
  QUE46_RS14425 (PspMM1_28120) ampD 3204852..3205406 (-) 555 WP_286245364.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUE46_RS14430 (PspMM1_28130) nadC 3205624..3206469 (+) 846 WP_286245365.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUE46_RS14435 (PspMM1_28140) pilA2 3206940..3207377 (+) 438 WP_286245366.1 pilin Machinery gene
  QUE46_RS14440 (PspMM1_28150) pilC 3207443..3208672 (+) 1230 WP_286245367.1 type II secretion system F family protein Machinery gene
  QUE46_RS14445 (PspMM1_28160) pilD 3208844..3209746 (+) 903 WP_286245368.1 A24 family peptidase Machinery gene
  QUE46_RS14450 (PspMM1_28170) coaE 3209748..3210368 (+) 621 WP_286245369.1 dephospho-CoA kinase -
  QUE46_RS14455 (PspMM1_28180) pilB 3210514..3212190 (+) 1677 WP_286245370.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14633.67 Da        Isoelectric Point: 6.1224

>NTDB_id=102843 QUE46_RS14435 WP_286245366.1 3206940..3207377(+) (pilA2) [Pseudoalteromonas sp. MM1]
MKTMTQKQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKVTEGLSLASAAKTTVTENAMNGNALDQAWVAPTATDNV
TSVGINGTTGVVTVTYTASAGNGTLTLTPTSGGAALASGTVPTGSVTWTCAAGTISTNYLPSSCR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=102843 QUE46_RS14435 WP_286245366.1 3206940..3207377(+) (pilA2) [Pseudoalteromonas sp. MM1]
ATGAAAACAATGACTCAAAAACAACAGGGTTTCACCCTAATCGAATTAATGATTGTTGTAGCAATCATCGGTATTCTTGC
TGCGGTGGCTTTGCCTGCTTACCAAGATTACACGATTAGAGCAAAGGTAACTGAAGGTTTATCGTTAGCTTCTGCAGCAA
AAACTACTGTTACTGAAAATGCTATGAATGGTAATGCTCTTGACCAAGCATGGGTGGCACCAACAGCCACAGACAACGTA
ACCTCAGTAGGAATTAACGGTACTACAGGGGTAGTAACCGTTACTTACACAGCATCTGCAGGTAATGGAACATTAACTTT
AACACCTACCTCTGGTGGTGCAGCTTTAGCATCTGGAACAGTTCCAACAGGCTCGGTAACTTGGACTTGTGCCGCTGGTA
CTATTTCAACAAATTACCTTCCTTCGAGCTGCAGATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

58.333

99.31

0.579
  pilA2 Legionella pneumophila strain ERS1305867

58.333

99.31

0.579
  pilA Ralstonia pseudosolanacearum GMI1000

50.303

100

0.572
  comP Acinetobacter baylyi ADP1

48.649

100

0.497
  pilE Neisseria gonorrhoeae MS11

40.964

100

0.469
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.233

100

0.469
  pilA/pilA1 Eikenella corrodens VA1

40.606

100

0.462
  pilE Neisseria gonorrhoeae strain FA1090

39.264

100

0.441
  pilA Acinetobacter baumannii strain A118

41.379

100

0.414
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.559

98.621

0.4
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.242

100

0.372
  pilA Vibrio parahaemolyticus RIMD 2210633

46.154

80.69

0.372

Multiple sequence alignment