Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUE46_RS14455 Genome accession   NZ_AP027922
Coordinates   3210514..3212190 (+) Length   558 a.a.
NCBI ID   WP_286245370.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. MM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3205514..3217190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE46_RS14430 (PspMM1_28130) nadC 3205624..3206469 (+) 846 WP_286245365.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUE46_RS14435 (PspMM1_28140) pilA2 3206940..3207377 (+) 438 WP_286245366.1 pilin Machinery gene
  QUE46_RS14440 (PspMM1_28150) pilC 3207443..3208672 (+) 1230 WP_286245367.1 type II secretion system F family protein Machinery gene
  QUE46_RS14445 (PspMM1_28160) pilD 3208844..3209746 (+) 903 WP_286245368.1 A24 family peptidase Machinery gene
  QUE46_RS14450 (PspMM1_28170) coaE 3209748..3210368 (+) 621 WP_286245369.1 dephospho-CoA kinase -
  QUE46_RS14455 (PspMM1_28180) pilB 3210514..3212190 (+) 1677 WP_286245370.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUE46_RS14460 (PspMM1_28190) yacG 3212243..3212473 (+) 231 WP_286245371.1 DNA gyrase inhibitor YacG -
  QUE46_RS14465 (PspMM1_28200) - 3212546..3213037 (-) 492 WP_286245372.1 Spy/CpxP family protein refolding chaperone -
  QUE46_RS14470 (PspMM1_28210) - 3213213..3213902 (+) 690 WP_004588608.1 response regulator -
  QUE46_RS14475 (PspMM1_28220) - 3213880..3215295 (+) 1416 WP_286245374.1 ATP-binding protein -
  QUE46_RS14480 (PspMM1_28230) - 3215299..3216246 (+) 948 WP_286245375.1 alpha/beta fold hydrolase -
  QUE46_RS14485 (PspMM1_28240) - 3216236..3217072 (-) 837 WP_286247781.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61979.18 Da        Isoelectric Point: 5.0828

>NTDB_id=102846 QUE46_RS14455 WP_286245370.1 3210514..3212190(+) (pilB) [Pseudoalteromonas sp. MM1]
MNINSPLLRKFITLGRIDADTVKAKQQEFASTAELISKCGKIDSNDLAEQCIDLFRVPYFDLKDFDPAQIPEDLVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDAAGSLDLSADEFKEFADLDTEG
PKETTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVANPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKMTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEDQKKLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKAEMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPAEELARQGFSTAQIEDMTL
YAPKGCENCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLQAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=102846 QUE46_RS14455 WP_286245370.1 3210514..3212190(+) (pilB) [Pseudoalteromonas sp. MM1]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACGCTTGGCCGCATTGATGCCGATACCGTAAAAGCAAAACAGCA
AGAGTTTGCCTCGACCGCCGAGCTTATATCTAAATGTGGAAAAATTGACAGCAACGACCTAGCTGAGCAATGCATAGACT
TATTTCGCGTACCGTATTTTGATTTAAAAGACTTCGACCCTGCACAAATCCCCGAAGACTTAGTTAAAGAAAAACTAATA
CGTAAACACCACATTTTACCGCTTGTTCAAAAAGGCAGAAAAGTATACATAGCCGCCTCAGATCCAACCGATTACGGTGC
CTTTGAAAACTTTGAATTTAGCACCGGTTTATCGTGCGAAATAGTCGTGGTTGACTACATTCAGCTGGATAATAAAATTG
AGCAACTACTTGATGCTGCAGGCAGCTTAGATTTAAGCGCCGATGAATTTAAAGAGTTTGCCGACTTAGATACTGAAGGC
CCTAAAGAAACCACACCTAAAGATGACGATAAAGACGATGCGCCCATCATTGTTTATATCAATAAAATTTTAATGGATGC
CATTAAAAAAGGCGCCTCTGATTTACACTTTGAACCTTACGAACATAAATACCGTATTCGCTTTCGTATTGATGGGATTT
TGCACGAAGTTGCAAATCCACCCAACACCTTAGCAAGCAAGCTATCGGCACGTATAAAAGTAATGTCACGCCTTGATATT
GCAGAAAAACGTAAACCACAAGATGGCCGTATTAAACTAAAAATGACCGAGCGTAAAAGCATCGACTTTCGTGTAAGTAC
CATGCCTACGTTATGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCTATGCTAGGTATTGACGTATTAG
GTTACGAAGAGGATCAGAAAAAGCTCTATATGGATGCGCTTGGGCAACCACAAGGTATGATACTTGTAACCGGCCCTACC
GGGTCGGGTAAAACAGTATCGCTTTATACCGGTTTAAATATACTTAACCAACCAGAGCGAAACATTAGTACCGCCGAGGA
TCCGGTAGAAATAAACCTTGAAGGGGTTAACCAAGTACAAATAAACCCTAAAGCCGAGATGACCTTTGCCAACGCCCTGC
GTGCCTTTTTACGTCAAGATCCTGATGTAGTGATGGTTGGTGAAATACGAGATCTAGAAACCGCAGAAATTTCAATTAAA
GCGGCGCAAACAGGTCACTTAGTGTTATCAACACTACATACCAACTCAGCCCCCGAAACCATAACGCGTTTATTAAATAT
GGGCGTGCCTGCGTATAACGTAGCAAGCTCTATAAGTTTGATTATTGCCCAGCGCTTAGCTAGGCGTTTATGCCCTAAAT
GTAAAACCCCAGAAACACTGCCTGCAGAAGAATTAGCTCGCCAAGGTTTTAGCACCGCGCAAATTGAAGACATGACTCTA
TACGCGCCCAAAGGCTGCGAAAATTGCACCGATGGTTACAAAGGCCGTGTAGGTATTTACGAAGTAATGCAAATTACCCC
TGAAATTGCACAAATTATTATGCGCGGTGGTAATTCACTCGAAATAGCAGAGGTATCACTGCAAGCTGGGTTTAATAACT
TACGATTATCGGGTTTACGCAAAGCCGCAGATGGTTTAACGTCTTTGGCTGAAATAAACCGCGTTACAAATATGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Acinetobacter baylyi ADP1

58.743

91.219

0.536
  pilB Legionella pneumophila strain ERS1305867

53.165

99.104

0.527
  pilB Vibrio cholerae strain A1552

52.919

95.161

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

52.632

95.341

0.502
  pilB Vibrio campbellii strain DS40M4

51.88

95.341

0.495
  pilF Neisseria gonorrhoeae MS11

49.042

93.548

0.459

Multiple sequence alignment