Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4Y39_RS20655 Genome accession   NZ_CP162607
Coordinates   4474904..4475932 (-) Length   342 a.a.
NCBI ID   WP_045181706.1    Uniprot ID   A0AB39B1X7
Organism   Pseudomonas sp. Hg7Tf     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4469904..4480932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4Y39_RS20635 (AB4Y39_20635) argS 4471147..4472883 (+) 1737 WP_045181717.1 arginine--tRNA ligase -
  AB4Y39_RS20640 (AB4Y39_20640) - 4472885..4473574 (+) 690 WP_045181714.1 SPOR domain-containing protein -
  AB4Y39_RS20645 (AB4Y39_20645) - 4473616..4474035 (-) 420 WP_045181711.1 TM2 domain-containing protein -
  AB4Y39_RS20650 (AB4Y39_20650) - 4474284..4474907 (+) 624 WP_045181709.1 C40 family peptidase -
  AB4Y39_RS20655 (AB4Y39_20655) pilT 4474904..4475932 (-) 1029 WP_045181706.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4Y39_RS20660 (AB4Y39_20660) - 4475999..4476685 (+) 687 WP_101293745.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4Y39_RS20665 (AB4Y39_20665) proC 4476724..4477542 (+) 819 WP_045181700.1 pyrroline-5-carboxylate reductase -
  AB4Y39_RS20670 (AB4Y39_20670) - 4477553..4478143 (+) 591 WP_280043085.1 YggT family protein -
  AB4Y39_RS20675 (AB4Y39_20675) - 4478296..4479435 (+) 1140 WP_045181694.1 homoserine O-acetyltransferase -
  AB4Y39_RS20680 (AB4Y39_20680) metW 4479443..4480063 (+) 621 WP_045181692.1 methionine biosynthesis protein MetW -
  AB4Y39_RS20685 (AB4Y39_20685) - 4480088..4480522 (+) 435 WP_101293743.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 37197.94 Da        Isoelectric Point: 6.5030

>NTDB_id=1027453 AB4Y39_RS20655 WP_045181706.1 4474904..4475932(-) (pilT) [Pseudomonas sp. Hg7Tf]
MDVTELLALAIREGASDLHVATDEVPAIRLDGRICRLQLPTLSAEQVQKMLAQLMSDEQKKDFETLLELDFSLSMPGLGR
FRVNAFNQLRGSGAVLRVIPRQVSSLAELGLGDVFRRIAELPSGLVLVTGPTGSGKSTTLAALLDHLNRERQLHILTLED
PIEFIHSPQRSLISQREIGRHSHGFTQALRAALRQDPDVIMLGELRDQESIRLALSAAETGHLVLATLHTVSAAKAIDRL
VDVFAAEEKQLVRAMLSEALQAVVAQVLVERVAGGRVAAHEILLATPAVRNLIREGKVAQLYSTMQMGGAVGMQTLEGCL
QGLSARGEIRPDCAREWAHGSF

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=1027453 AB4Y39_RS20655 WP_045181706.1 4474904..4475932(-) (pilT) [Pseudomonas sp. Hg7Tf]
ATGGATGTGACTGAACTGCTGGCCCTGGCAATAAGGGAAGGGGCTTCGGACCTGCACGTGGCCACCGATGAGGTGCCAGC
GATTCGTTTGGACGGGCGTATTTGCCGCCTGCAGCTGCCGACGTTGAGCGCTGAGCAGGTGCAGAAGATGCTCGCGCAGC
TCATGTCCGATGAGCAAAAAAAGGATTTTGAAACACTCTTGGAACTCGATTTCTCACTGTCCATGCCCGGACTTGGTCGT
TTTCGCGTCAATGCCTTTAACCAGTTGCGTGGCTCAGGGGCGGTGCTGCGGGTCATTCCTCGTCAGGTGAGTAGCCTGGC
CGAATTGGGCTTGGGTGACGTGTTTCGGCGGATTGCCGAGTTGCCCAGCGGCCTGGTGCTGGTTACAGGGCCTACTGGTT
CGGGCAAATCCACCACGTTGGCAGCGCTGCTGGATCATCTGAACCGTGAGCGGCAGTTACACATTCTCACGTTGGAAGAC
CCCATCGAATTTATCCACAGCCCGCAGCGCAGCCTGATCAGTCAACGCGAGATCGGCAGGCACAGTCATGGTTTTACCCA
GGCGCTACGCGCAGCGCTGCGTCAGGATCCGGATGTGATCATGCTCGGTGAGCTGCGCGACCAGGAGAGTATCCGTCTGG
CCCTCAGTGCTGCTGAAACGGGGCACTTGGTGCTGGCGACCCTGCACACGGTATCGGCCGCAAAGGCGATCGATCGCCTG
GTGGATGTGTTTGCCGCTGAGGAGAAGCAACTAGTGCGCGCCATGCTCAGCGAAGCGTTGCAGGCGGTGGTGGCCCAGGT
GCTGGTCGAGCGAGTCGCTGGTGGCCGGGTGGCAGCCCATGAAATTTTGCTGGCCACGCCTGCCGTGCGCAATCTGATCC
GCGAAGGAAAGGTCGCGCAACTGTACTCGACGATGCAGATGGGTGGGGCGGTGGGCATGCAGACCCTGGAGGGCTGCCTG
CAAGGCCTGTCAGCGCGTGGGGAGATCAGGCCAGACTGCGCCCGTGAATGGGCGCATGGCAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

61.243

98.83

0.605
  pilT Pseudomonas aeruginosa PAK

60.947

98.83

0.602
  pilT Acinetobacter baylyi ADP1

59.172

98.83

0.585
  pilT Acinetobacter baumannii D1279779

59.172

98.83

0.585
  pilT Acinetobacter baumannii strain A118

59.172

98.83

0.585
  pilT Acinetobacter nosocomialis M2

58.876

98.83

0.582
  pilT Neisseria meningitidis 8013

55.263

100

0.553
  pilT Neisseria gonorrhoeae MS11

54.971

100

0.55
  pilT Vibrio cholerae strain A1552

56.269

95.614

0.538
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.269

95.614

0.538
  pilT Legionella pneumophila strain ERS1305867

54.142

98.83

0.535
  pilT Legionella pneumophila strain Lp02

54.142

98.83

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.737

99.123

0.503
  pilU Pseudomonas stutzeri DSM 10701

40.606

96.491

0.392
  pilU Vibrio cholerae strain A1552

38.788

96.491

0.374

Multiple sequence alignment