Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4P94_RS08365 Genome accession   NZ_CP162592
Coordinates   1840510..1841544 (+) Length   344 a.a.
NCBI ID   WP_395610161.1    Uniprot ID   -
Organism   Pseudomonas sp. B22129     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1835510..1846544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P94_RS08335 (AB4P94_08320) - 1835872..1836306 (-) 435 WP_395610157.1 DUF4426 domain-containing protein -
  AB4P94_RS08340 (AB4P94_08325) metW 1836328..1836948 (-) 621 WP_016972957.1 methionine biosynthesis protein MetW -
  AB4P94_RS08345 (AB4P94_08330) - 1836956..1838095 (-) 1140 WP_395610158.1 homoserine O-acetyltransferase -
  AB4P94_RS08350 (AB4P94_08335) - 1838302..1838889 (-) 588 WP_078739571.1 YggT family protein -
  AB4P94_RS08355 (AB4P94_08340) proC 1838914..1839732 (-) 819 WP_395610159.1 pyrroline-5-carboxylate reductase -
  AB4P94_RS08360 (AB4P94_08345) - 1839766..1840452 (-) 687 WP_395610160.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4P94_RS08365 (AB4P94_08350) pilT 1840510..1841544 (+) 1035 WP_395610161.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4P94_RS08370 (AB4P94_08355) - 1841691..1842329 (-) 639 WP_016972951.1 C40 family peptidase -
  AB4P94_RS08375 (AB4P94_08360) - 1842579..1843007 (+) 429 WP_395610162.1 NINE protein -
  AB4P94_RS08380 (AB4P94_08365) - 1843143..1844414 (-) 1272 WP_395610163.1 dihydroorotase -
  AB4P94_RS08385 (AB4P94_08370) - 1844411..1845415 (-) 1005 WP_395610164.1 aspartate carbamoyltransferase catalytic subunit -
  AB4P94_RS08390 (AB4P94_08375) pyrR 1845440..1845946 (-) 507 WP_395610165.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AB4P94_RS08395 (AB4P94_08380) ruvX 1846020..1846457 (-) 438 WP_003195067.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37395.14 Da        Isoelectric Point: 6.8430

>NTDB_id=1027314 AB4P94_RS08365 WP_395610161.1 1840510..1841544(+) (pilT) [Pseudomonas sp. B22129]
MDITELLTTGVRRGASDLHLSAGLVPMLRVDGEVWPLDGPILSPTQVADLLSPLLNLHQQKDFETSLETDFAFELPGVGR
FRANVFQQDRGMGAVFRTIPAQVQSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNRHRRYHILTLED
PIEFIHRPKTALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLEAVVSQVLVKKIGGGRVAAHEIMLSTPAIRNLIREDKGAQMVSAIQTGGALGMKTLDMSL
KALVGEGLISREDARDKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1027314 AB4P94_RS08365 WP_395610161.1 1840510..1841544(+) (pilT) [Pseudomonas sp. B22129]
ATGGATATCACTGAATTGCTGACGACAGGCGTGCGCCGTGGCGCGTCCGACCTGCATTTGTCGGCTGGCCTGGTACCGAT
GCTGAGAGTCGATGGCGAGGTCTGGCCACTGGACGGCCCGATACTTTCACCTACGCAAGTTGCGGATTTATTGAGCCCAT
TGCTCAATCTGCACCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGCTGCCCGGAGTGGGGCGG
TTTCGCGCCAATGTGTTTCAGCAGGATCGTGGCATGGGAGCGGTGTTTCGCACCATACCGGCGCAAGTGCAGAGCCTGGA
AAGCCTCGGGCTGGGCGAGGTTTTCCAACGGATCGCCCAGTTGCCGCGCGGGCTGGTATTGGTCACCGGGCCTACCGGCT
CCGGCAAGTCCACGACGCTGGCGGCGATGATCGATTTTCTCAATCGGCATCGGCGTTATCACATCCTCACACTGGAAGAC
CCCATCGAGTTTATCCACAGGCCCAAAACGGCGCTGATCAACCAGCGCCAGGTGCATCGGGATACCCACAGTTTTTCAGC
GGCTCTGCGTTCGGCCTTGCGTGAAGATCCGGATGTAATCCTGGTGGGTGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCTGAGACCGGGCACCTGGTATTTGGCACCCTGCACACCTCGTCGGCCGCAAAGACTGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAAAAAGCCATGGTGCGCTCGATGCTGTCGGAGTCGCTAGAGGCGGTGGTGTCTCAGGT
ATTGGTGAAGAAGATTGGCGGCGGGCGCGTGGCGGCCCATGAAATCATGCTGAGCACGCCGGCGATTCGGAATTTGATCC
GCGAGGACAAGGGCGCGCAGATGGTCTCGGCGATCCAGACCGGTGGGGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGCTGGTGGGCGAGGGGCTGATCAGCCGGGAAGATGCGCGGGATAAGGCGAGGGTGCCCGCAGATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

69.767

100

0.698
  pilT Acinetobacter baumannii D1279779

68.328

99.128

0.677
  pilT Acinetobacter baumannii strain A118

68.328

99.128

0.677
  pilT Acinetobacter nosocomialis M2

68.328

99.128

0.677
  pilT Acinetobacter baylyi ADP1

68.035

99.128

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.095

97.965

0.628
  pilT Vibrio cholerae strain A1552

64.095

97.965

0.628
  pilT Legionella pneumophila strain Lp02

63.018

98.256

0.619
  pilT Legionella pneumophila strain ERS1305867

63.018

98.256

0.619
  pilT Neisseria meningitidis 8013

62.353

98.837

0.616
  pilT Neisseria gonorrhoeae MS11

62.059

98.837

0.613
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment