Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4P96_RS03785 Genome accession   NZ_CP162589
Coordinates   849396..850430 (+) Length   344 a.a.
NCBI ID   WP_017139451.1    Uniprot ID   -
Organism   Pseudomonas sp. B19125     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 844396..855430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P96_RS03755 (AB4P96_03755) - 844766..845203 (-) 438 WP_017139456.1 DUF4426 domain-containing protein -
  AB4P96_RS03760 (AB4P96_03760) metW 845225..845845 (-) 621 WP_016972957.1 methionine biosynthesis protein MetW -
  AB4P96_RS03765 (AB4P96_03765) - 845853..846992 (-) 1140 WP_170036803.1 homoserine O-acetyltransferase -
  AB4P96_RS03770 (AB4P96_03770) - 847187..847774 (-) 588 WP_017139454.1 YggT family protein -
  AB4P96_RS03775 (AB4P96_03775) proC 847800..848618 (-) 819 WP_395600514.1 pyrroline-5-carboxylate reductase -
  AB4P96_RS03780 (AB4P96_03780) - 848652..849338 (-) 687 WP_169987578.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4P96_RS03785 (AB4P96_03785) pilT 849396..850430 (+) 1035 WP_017139451.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4P96_RS03790 (AB4P96_03790) - 850561..851196 (-) 636 WP_017139450.1 C40 family peptidase -
  AB4P96_RS03795 (AB4P96_03795) - 851446..851874 (+) 429 WP_138454259.1 TM2 domain-containing protein -
  AB4P96_RS03800 (AB4P96_03800) - 852020..853291 (-) 1272 WP_099550338.1 dihydroorotase -
  AB4P96_RS03805 (AB4P96_03805) - 853288..854292 (-) 1005 WP_056857699.1 aspartate carbamoyltransferase catalytic subunit -
  AB4P96_RS03810 (AB4P96_03810) pyrR 854317..854823 (-) 507 WP_017139446.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AB4P96_RS03815 (AB4P96_03815) ruvX 854906..855343 (-) 438 WP_003195067.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37274.15 Da        Isoelectric Point: 7.7199

>NTDB_id=1027255 AB4P96_RS03785 WP_017139451.1 849396..850430(+) (pilT) [Pseudomonas sp. B19125]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEIWPLEGPVLSPTQVADLLSPLLNQHQQKDFETSLETDFAFALPGLAR
FRANVFRQDRGMGAVFRTIPTEVQSLESLGLGEVFRRIAQLPRGLVLVTGATGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKSVDRL
VDVFPAGEKAMVRAMLSESLQAVVSQVLVKKLGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVSAGLISREDARGLARVPTDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1027255 AB4P96_RS03785 WP_017139451.1 849396..850430(+) (pilT) [Pseudomonas sp. B19125]
ATGGATATCACTGAACTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCGGCTGGCCTGGCGCCGAT
GCTGCGTGTCGATGGCGAAATCTGGCCATTGGAGGGGCCGGTGCTTTCGCCTACGCAAGTTGCGGACTTATTAAGCCCTT
TGCTCAACCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCGTTCGCACTGCCGGGCCTGGCGCGG
TTCCGCGCGAATGTGTTCCGCCAGGATCGCGGAATGGGCGCGGTGTTTCGCACCATCCCGACCGAAGTGCAAAGCCTGGA
GAGCCTCGGGTTAGGTGAGGTATTCCGCCGTATCGCACAGCTGCCGCGTGGCCTGGTACTGGTGACCGGGGCGACCGGCT
CCGGCAAGTCGACGACCCTGGCGGCGATGATCGACCACCTCAACCAGCACCGGCGCCAGCACATCCTCACGCTGGAAGAC
CCGATCGAATTTATCCACAGGCCGAAAATGGCCCTGATCAACCAGCGCCAGGTACACCGCGACACTCATAGTTTTTCGGC
GGCGCTGCGCTCGGCCCTGCGTGAAGACCCGGATGTGATTCTGGTGGGCGAGCTGCGTGACCTGGAAACCATCCGCCTGG
CGTTGACGGCCGCAGAGACCGGGCACTTGGTATTGGGCACGCTGCACACCACCTCAGCGGCAAAGAGCGTGGATCGGCTG
GTGGACGTGTTCCCGGCGGGGGAAAAAGCCATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCGGTGGTGTCCCAGGT
GCTGGTCAAGAAGCTCGGCGGCGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACGCCAGCCATCCGTAACCTGATCC
GCGAGGACAAAGTGGCGCAGATGGTCTCGGCGATTCAAACCGGCGGGGCGTTGGGGATGAAAACGCTGGATATGAGCTTG
AAGGCGTTGGTCAGCGCAGGGCTGATCAGCCGGGAGGATGCGCGGGGGCTGGCGCGGGTGCCGACAGATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.221

100

0.712
  pilT Pseudomonas stutzeri DSM 10701

69.477

100

0.695
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baumannii D1279779

68.622

99.128

0.68
  pilT Acinetobacter baumannii strain A118

68.622

99.128

0.68
  pilT Acinetobacter baylyi ADP1

68.622

99.128

0.68
  pilT Legionella pneumophila strain Lp02

63.473

97.093

0.616
  pilT Legionella pneumophila strain ERS1305867

63.473

97.093

0.616
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.202

97.674

0.608
  pilT Vibrio cholerae strain A1552

62.202

97.674

0.608
  pilT Neisseria meningitidis 8013

61.012

97.674

0.596
  pilT Neisseria gonorrhoeae MS11

60.714

97.674

0.593
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.176

98.837

0.506
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment