Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4P95_RS24135 Genome accession   NZ_CP162583
Coordinates   5224339..5225373 (+) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas sp. A1437     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5219339..5230373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P95_RS24100 (AB4P95_24060) - 5219456..5219890 (-) 435 WP_042561549.1 DUF4426 domain-containing protein -
  AB4P95_RS24105 (AB4P95_24065) metW 5219909..5220529 (-) 621 WP_042561548.1 methionine biosynthesis protein MetW -
  AB4P95_RS24110 (AB4P95_24070) - 5220537..5221676 (-) 1140 WP_042561547.1 homoserine O-acetyltransferase -
  AB4P95_RS24115 (AB4P95_24075) - 5221863..5222156 (-) 294 WP_042561546.1 DUF167 domain-containing protein -
  AB4P95_RS24120 (AB4P95_24080) - 5222153..5222743 (-) 591 WP_042561545.1 YggT family protein -
  AB4P95_RS24125 (AB4P95_24085) proC 5222754..5223572 (-) 819 WP_042561544.1 pyrroline-5-carboxylate reductase -
  AB4P95_RS24130 (AB4P95_24090) - 5223596..5224282 (-) 687 WP_395597941.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4P95_RS24135 (AB4P95_24095) pilT 5224339..5225373 (+) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4P95_RS24140 (AB4P95_24100) - 5225481..5226098 (-) 618 WP_042561542.1 C40 family peptidase -
  AB4P95_RS24145 (AB4P95_24105) - 5226347..5226781 (+) 435 WP_042561541.1 TM2 domain-containing protein -
  AB4P95_RS24150 (AB4P95_24110) - 5226965..5228236 (-) 1272 WP_395597942.1 dihydroorotase -
  AB4P95_RS24155 (AB4P95_24115) - 5228233..5229237 (-) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  AB4P95_RS24160 (AB4P95_24120) pyrR 5229265..5229771 (-) 507 WP_042561538.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AB4P95_RS24165 (AB4P95_24125) ruvX 5229854..5230291 (-) 438 WP_042561537.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=1027215 AB4P95_RS24135 WP_007953348.1 5224339..5225373(+) (pilT) [Pseudomonas sp. A1437]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1027215 AB4P95_RS24135 WP_007953348.1 5224339..5225373(+) (pilT) [Pseudomonas sp. A1437]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCACTGGACCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAATGCCTTCAATCAGAATCGTGGCGCGGGTGCCGTGTTTCGCACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTCCGCAAGATCACCGATGCTCCGCGCGGGTTGGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACCTCAACACCCATCGCCACCATCACATCCTGACCATCGAAGAC
CCGATCGAGTTTGTTCACGAGTCGCGCAAATGCCTGATCAATCAGCGTGAAGTTCACCGCGATACCCGAAGTTTCGCCAC
GGCTCTGCGTTCGGCATTGCGAGAAGACCCGGACGTGATTCTGGTGGGCGAGATGCGCGACCTGGAGACCATTCGCCTGG
CGCTGACTGCCGCCGAGACCGGGCACCTGGTATTCGGCACGCTGCACACGACGTCTGCCGCGAAAACCATCGACCGTGTG
GTGGACGTGTTCCCCGGTGACGAGAAATCCATGGTGCGTTCGATGCTGTCGGAGTCACTGCTGGCAGTGGTCTCGCAGAC
GTTGATCAAGAAGATCGGCGGCGGGCGTGTGGCGGCGCATGAGATCATGCTGGGAACGTCAGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGTGGGTCGCTGGGGATGCAGACACTGGATATGTGCCTG
AAAGATCTGGTGACCAAGGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment