Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4P97_RS21715 Genome accession   NZ_CP162582
Coordinates   4637024..4638058 (-) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas sp. A1230     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4632024..4643058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P97_RS21695 (AB4P97_21640) - 4632486..4633757 (+) 1272 WP_273878335.1 dihydroorotase -
  AB4P97_RS21700 (AB4P97_21645) - 4634114..4635100 (+) 987 WP_395601600.1 lipid II-degrading bacteriocin -
  AB4P97_RS21705 (AB4P97_21650) - 4635613..4636047 (-) 435 WP_150601974.1 TM2 domain-containing protein -
  AB4P97_RS21710 (AB4P97_21655) - 4636296..4636913 (+) 618 WP_042561542.1 C40 family peptidase -
  AB4P97_RS21715 (AB4P97_21660) pilT 4637024..4638058 (-) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4P97_RS21720 (AB4P97_21665) - 4638115..4638801 (+) 687 WP_042561543.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4P97_RS21725 (AB4P97_21670) proC 4638825..4639643 (+) 819 WP_395601601.1 pyrroline-5-carboxylate reductase -
  AB4P97_RS21730 (AB4P97_21675) - 4639654..4640244 (+) 591 WP_042561545.1 YggT family protein -
  AB4P97_RS21735 (AB4P97_21680) - 4640241..4640534 (+) 294 WP_042561546.1 DUF167 domain-containing protein -
  AB4P97_RS21740 (AB4P97_21685) - 4640721..4641860 (+) 1140 WP_042561547.1 homoserine O-acetyltransferase -
  AB4P97_RS21745 (AB4P97_21690) metW 4641868..4642488 (+) 621 WP_042561548.1 methionine biosynthesis protein MetW -
  AB4P97_RS21750 (AB4P97_21695) - 4642507..4642941 (+) 435 WP_042561549.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=1027192 AB4P97_RS21715 WP_007953348.1 4637024..4638058(-) (pilT) [Pseudomonas sp. A1230]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1027192 AB4P97_RS21715 WP_007953348.1 4637024..4638058(-) (pilT) [Pseudomonas sp. A1230]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCACTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAATGCCTTCAATCAGAATCGTGGCGCGGGTGCCGTGTTCCGCACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTCCGCAAGATCACCGATGCTCCGCGCGGGTTGGTGCTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACCTCAACACCCATCGCCACCATCACATCCTGACCATCGAAGAC
CCGATCGAGTTTGTTCACGAGTCGCGCAAATGCTTGATCAATCAGCGTGAAGTCCACCGCGATACCCGTAGTTTTGCCAC
GGCTCTGCGTTCAGCGCTGCGTGAAGACCCGGACGTGATTCTGGTGGGCGAGATGCGCGACCTGGAGACCATTCGCCTGG
CGTTGACTGCCGCCGAGACCGGGCACCTGGTATTCGGCACGCTGCACACGACGTCTGCCGCGAAAACCATCGACCGTGTG
GTGGACGTGTTCCCCGGTGACGAGAAATCCATGGTGCGTTCGATGCTGTCGGAGTCACTGCTGGCAGTGGTCTCGCAGAC
GTTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCACGAGATCATGCTGGGAACGTCAGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGTGGGTCGCTGGGGATGCAGACACTGGATATGTGCCTG
AAAGATCTGGTGACCAAGGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment