Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AB3X10_RS05760 Genome accession   NZ_CP162487
Coordinates   1354042..1354476 (+) Length   144 a.a.
NCBI ID   WP_369979839.1    Uniprot ID   -
Organism   Xanthomonas bundabergensis strain DAR 80977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1349042..1359476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3X10_RS05740 (AB3X10_05730) - 1349449..1350783 (+) 1335 WP_369979833.1 sensor histidine kinase -
  AB3X10_RS05745 (AB3X10_05735) coaE 1350896..1351516 (-) 621 WP_369979834.1 dephospho-CoA kinase -
  AB3X10_RS05750 (AB3X10_05740) - 1351529..1352392 (-) 864 WP_179566660.1 prepilin peptidase -
  AB3X10_RS05755 (AB3X10_05745) pilC 1352399..1353661 (-) 1263 WP_369979837.1 type II secretion system F family protein Machinery gene
  AB3X10_RS05760 (AB3X10_05750) pilA 1354042..1354476 (+) 435 WP_369979839.1 pilin Machinery gene
  AB3X10_RS05765 (AB3X10_05755) pilB 1355299..1357035 (+) 1737 WP_369979840.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3X10_RS05770 (AB3X10_05760) pilR 1357200..1358588 (-) 1389 WP_369979842.1 sigma-54-dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14813.05 Da        Isoelectric Point: 9.4152

>NTDB_id=1026411 AB3X10_RS05760 WP_369979839.1 1354042..1354476(+) (pilA) [Xanthomonas bundabergensis strain DAR 80977]
MKKQQGFTLIELMIVVAIIAILAAIAIPAYNDYVTRAQVSEAVSLAGGLKAPLAEYGANANAWPTLVAPTATATSTQIPA
TLTGKYATISNAITGTYPAGTITATMTSGRANGTTILFVTPDGGATWTCNTGTALSKYRPQACR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1026411 AB3X10_RS05760 WP_369979839.1 1354042..1354476(+) (pilA) [Xanthomonas bundabergensis strain DAR 80977]
ATGAAAAAGCAGCAGGGCTTCACGCTGATTGAACTTATGATCGTGGTCGCGATCATCGCCATTCTGGCCGCTATCGCTAT
TCCGGCGTACAACGACTATGTAACCAGGGCGCAAGTGTCTGAGGCAGTTTCGCTTGCCGGTGGACTCAAGGCTCCGCTTG
CCGAGTATGGTGCCAATGCCAATGCTTGGCCGACTCTGGTAGCGCCTACGGCAACCGCAACGTCGACGCAGATTCCAGCT
ACTCTCACCGGCAAGTACGCCACGATCTCCAATGCGATCACTGGCACGTATCCGGCAGGAACCATTACCGCTACTATGAC
TAGCGGCCGCGCAAATGGTACTACGATCCTGTTTGTAACCCCGGATGGCGGCGCTACCTGGACCTGCAATACCGGCACCG
CTCTCTCCAAGTACCGTCCGCAGGCTTGTCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

41.42

100

0.486
  comP Acinetobacter baylyi ADP1

45.033

100

0.472
  pilA2 Legionella pneumophila str. Paris

47.222

100

0.472
  pilA2 Legionella pneumophila strain ERS1305867

45.139

100

0.451
  pilA/pilA1 Eikenella corrodens VA1

38.816

100

0.41
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.889

100

0.389
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.095

100

0.389
  pilA Pseudomonas aeruginosa PAK

36.424

100

0.382
  pilA Acinetobacter baumannii strain A118

38.194

100

0.382
  pilA Acinetobacter nosocomialis M2

46.552

80.556

0.375
  pilE Neisseria gonorrhoeae MS11

40.909

91.667

0.375
  pilE Neisseria gonorrhoeae strain FA1090

40.909

91.667

0.375
  pilA Vibrio parahaemolyticus RIMD 2210633

41.085

89.583

0.368
  comF Acinetobacter baylyi ADP1

38.519

93.75

0.361

Multiple sequence alignment