Detailed information NTG1026412

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: AB3X10_RS05765
• Genome accession: NZ_CP162487
• Coordinates: 1355299..1357035 (+)
• Length: 578 a.a.
• NCBI ID: WP_369979840.1
• Uniprot ID: -
• Organism: Xanthomonas bundabergensis strain DAR 80977
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1350299..1362035

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3X10_RS05745 (AB3X10_05735)	coaE	1350896..1351516 (-)	621	WP_369979834.1	dephospho-CoA kinase	-
AB3X10_RS05750 (AB3X10_05740)	-	1351529..1352392 (-)	864	WP_179566660.1	prepilin peptidase	-
AB3X10_RS05755 (AB3X10_05745)	pilC	1352399..1353661 (-)	1263	WP_369979837.1	type II secretion system F family protein	Machinery gene
AB3X10_RS05760 (AB3X10_05750)	pilA	1354042..1354476 (+)	435	WP_369979839.1	pilin	Machinery gene
AB3X10_RS05765 (AB3X10_05755)	pilB	1355299..1357035 (+)	1737	WP_369979840.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AB3X10_RS05770 (AB3X10_05760)	pilR	1357200..1358588 (-)	1389	WP_369979842.1	sigma-54-dependent transcriptional regulator	Regulator
AB3X10_RS05775 (AB3X10_05765)	-	1358663..1360276 (-)	1614	WP_369979844.1	sensor histidine kinase	-
AB3X10_RS05780 (AB3X10_05770)	sucC	1360576..1361739 (+)	1164	WP_369979846.1	ADP-forming succinate--CoA ligase subunit beta	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62793.91 Da
• Isoelectric Point: 5.7881

>NTDB_id=1026412 AB3X10_RS05765 WP_369979840.1 1355299..1357035(+) (pilB) [Xanthomonas bundabergensis strain DAR 80977]
MNALTSANLVGITGIARRLVQDGVLDESLARTAMAHAAEAKIPLPQWFSEKKLVTAAQLAAANAVEFGMPLLDVSVFDAN
QSAIKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGNSLG
GDDEGMDNLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
NNALLAEGFTEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDDIGAIVLQGGNAMDIAEAAQKIGVKDLRQSALL
KARNGITSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=1026412 AB3X10_RS05765 WP_369979840.1 1355299..1357035(+) (pilB) [Xanthomonas bundabergensis strain DAR 80977]
ATGAATGCTCTGACATCCGCCAACCTGGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGTGCTGGACGA
AAGTCTCGCGCGCACCGCCATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAATGGTTTTCCGAGAAGAAGCTGG
TCACCGCGGCGCAGCTGGCTGCCGCCAATGCGGTGGAGTTCGGGATGCCGCTGCTGGACGTGTCGGTGTTCGATGCCAAC
CAGAGCGCAATCAAGCTGGTCAGCGAGGAGCTGCTGCAGAAGCACCAGGTGCTGCCGTTGTTCAAGCGCGGCAACCGGCT
GTTCGTGGGGGTCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCCAGCAACGATGCGATCGGCAACAGCCTGGGC
GGCGACGACGAGGGCATGGACAACCTCGAGGTCGGGGCCGGCGACGAGGACATGGGCAGCGGCGGCGATACCGGTGTCGA
TGCCAAGGGCGACGACACGCCCGTGGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCGTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTACTCAAGAGCGTGGCCAAGGCGCCG
GTCAAGCTCAACCAGCGCATCGCGGCGCGGTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
TGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACGGCCGAGGACCCGGTCGAAATCCGCTTGCCCG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCCGCCTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGCTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCTCGGCGGCTGTGCAACAACTGCAAGCGCCCCACCCAGCTGCCG
AACAATGCGCTGCTGGCCGAAGGCTTCACCGAGGCCGAAGTCGCCGCCGGCATCCAACTGTACGAAGCGGTTGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGATATCGGAGCGATCGTGC
TGCAGGGCGGCAACGCGATGGATATCGCCGAGGCGGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTGCTC
AAGGCGCGCAACGGCATCACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (50-152)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	54.483	100	0.547
pilB	Acinetobacter baumannii D1279779	54.816	98.789	0.542
pilB	Legionella pneumophila strain ERS1305867	52.9	98.443	0.521
pilB	Vibrio cholerae strain A1552	48.696	99.481	0.484
pilF	Neisseria gonorrhoeae MS11	48.944	98.27	0.481
pilB	Vibrio campbellii strain DS40M4	46.749	98.443	0.46
pilB	Vibrio parahaemolyticus RIMD 2210633	46.316	98.616	0.457