Detailed information NTG1024194

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: AB1S56_RS10295
• Genome accession: NZ_CP162129
• Coordinates: 2457153..2458196 (+)
• Length: 347 a.a.
• NCBI ID: WP_340870725.1
• Uniprot ID: -
• Organism: Paenibacillus sp. PL2-23
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2452153..2463196

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB1S56_RS10290	gspE	2455437..2457101 (+)	1665	WP_340870724.1	type II secretion system ATPase GspE	-
AB1S56_RS10295	pilT	2457153..2458196 (+)	1044	WP_340870725.1	type IV pilus twitching motility protein PilT	Machinery gene
AB1S56_RS10300	-	2458196..2459404 (+)	1209	WP_340870726.1	type II secretion system F family protein	-
AB1S56_RS10305	-	2459479..2460000 (+)	522	WP_340870727.1	type II secretion system protein	-
AB1S56_RS10310	-	2460077..2460823 (+)	747	WP_340870728.1	prepilin peptidase	-
AB1S56_RS10315	tnpB	2460980..2462068 (+)	1089	WP_367903461.1	IS200/IS605 family element RNA-guided endonuclease TnpB	-

Sequence

Protein

• Length: 347 a.a.
• Molecular weight: 37447.86 Da
• Isoelectric Point: 6.7761

>NTDB_id=1024194 AB1S56_RS10295 WP_340870725.1 2457153..2458196(+) (pilT) [Paenibacillus sp. PL2-23]
MSSIETIIGLLQGAREQKASDLHISVGSPPSYRIDGALKSAADNKVTAEEAEGYAAALVTEAQRLQLEERGEVDFSYSLP
DCRFRMNIYRQQGYVSIAARLIAQSIPTLEQLQMPAVIAPLADKAQGLILVTGPTGSGKSSTLAAFIHHINRSQKKHIIT
LEDPIEYVHRSEASIIDQREVGQDTRSFSNGLRAALRQDPDVILVGEMRDLETISAAITAAETGHLVLGTLHTTDAPQTI
DRIIDAFPGPQQAQVRAQLAAVLLTVISQRLLPRSSGQGRIAATEILMNTPAAANLIRSEKVHQIRSLMQTGKALGMHTL
DMSIKELLQKGIVTPAAAKPYLTEGGL

Nucleotide

• Length: 1044 bp

>NTDB_id=1024194 AB1S56_RS10295 WP_340870725.1 2457153..2458196(+) (pilT) [Paenibacillus sp. PL2-23]
ATGTCGTCAATAGAAACGATTATTGGATTATTGCAAGGCGCGAGGGAGCAGAAGGCGTCTGACCTTCATATTTCGGTAGG
GTCGCCGCCTTCCTATCGGATTGACGGCGCGTTGAAGTCGGCGGCGGACAACAAGGTTACCGCGGAGGAAGCGGAAGGCT
ATGCGGCCGCTCTCGTGACGGAGGCTCAGCGTTTGCAGCTGGAGGAGCGGGGAGAGGTAGATTTTTCTTATTCACTTCCG
GACTGCAGATTCCGAATGAATATATATAGGCAGCAAGGCTATGTGAGCATCGCCGCAAGGCTGATTGCCCAGAGCATCCC
GACGCTGGAGCAGCTGCAGATGCCTGCAGTTATCGCTCCTCTTGCCGATAAGGCGCAAGGGCTTATTCTTGTGACGGGTC
CAACAGGCAGCGGGAAGTCTTCCACGCTTGCGGCGTTTATCCATCATATTAACCGCTCGCAGAAGAAGCATATCATTACG
CTGGAGGACCCCATCGAGTACGTTCACCGCAGCGAGGCGTCCATTATTGACCAGCGGGAGGTCGGCCAGGATACCAGAAG
CTTCTCGAACGGCTTGCGAGCCGCGCTGAGGCAGGATCCCGATGTTATTCTTGTAGGCGAGATGAGGGATCTGGAGACGA
TTTCTGCCGCTATAACAGCTGCTGAGACGGGGCATCTGGTGCTTGGAACACTGCATACGACCGACGCGCCGCAGACGATC
GACCGCATTATTGACGCTTTCCCGGGCCCGCAGCAGGCTCAGGTTCGCGCACAGCTTGCCGCCGTCCTGCTGACCGTTAT
ATCGCAGAGGCTGCTGCCGAGGTCATCCGGACAGGGACGGATTGCCGCAACCGAAATCCTGATGAACACGCCGGCTGCGG
CCAACCTGATCCGCTCCGAGAAGGTCCATCAGATCCGAAGTCTTATGCAGACGGGCAAAGCGCTGGGCATGCATACGCTG
GATATGTCCATCAAGGAGCTGCTGCAGAAAGGAATCGTGACACCGGCAGCCGCTAAGCCGTATCTGACGGAAGGAGGTCT
GTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (16-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	51.524	94.524	0.487
pilT	Vibrio cholerae strain A1552	51.524	94.524	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.552	96.542	0.478
pilT	Acinetobacter baylyi ADP1	48.802	96.254	0.47
pilT	Pseudomonas stutzeri DSM 10701	48.802	96.254	0.47
pilT	Pseudomonas aeruginosa PAK	48.503	96.254	0.467
pilT	Neisseria gonorrhoeae MS11	48.503	96.254	0.467
pilT	Neisseria meningitidis 8013	48.503	96.254	0.467
pilT	Legionella pneumophila strain Lp02	47.748	95.965	0.458
pilT	Legionella pneumophila strain ERS1305867	47.748	95.965	0.458
pilT	Acinetobacter baumannii strain A118	47.605	96.254	0.458
pilT	Acinetobacter nosocomialis M2	47.605	96.254	0.458
pilT	Acinetobacter baumannii D1279779	47.605	96.254	0.458
pilU	Pseudomonas stutzeri DSM 10701	41.411	93.948	0.389
pilU	Acinetobacter baylyi ADP1	39.275	95.389	0.375
pilU	Vibrio cholerae strain A1552	39.308	91.643	0.36