Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DUT67_RS04085 Genome accession   NZ_CP161826
Coordinates   905959..906975 (+) Length   338 a.a.
NCBI ID   WP_048262112.1    Uniprot ID   -
Organism   Pectobacterium peruviense strain A350-S18-N16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 900959..911975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DUT67_RS04050 (DUT67_04050) - 901379..901633 (-) 255 WP_223254640.1 type II toxin-antitoxin system RelE/ParE family toxin -
  DUT67_RS04055 (DUT67_04055) hemW 901675..902814 (-) 1140 WP_113632851.1 radical SAM family heme chaperone HemW -
  DUT67_RS04060 (DUT67_04060) - 902807..903400 (-) 594 WP_113632805.1 XTP/dITP diphosphatase -
  DUT67_RS04065 (DUT67_04065) yggU 903418..903708 (-) 291 WP_005974847.1 DUF167 family protein YggU -
  DUT67_RS04070 (DUT67_04070) - 903705..904259 (-) 555 WP_010295003.1 YggT family protein -
  DUT67_RS04075 (DUT67_04075) proC 904309..905130 (-) 822 WP_048262138.1 pyrroline-5-carboxylate reductase -
  DUT67_RS04080 (DUT67_04080) - 905224..905937 (-) 714 WP_048262115.1 YggS family pyridoxal phosphate-dependent enzyme -
  DUT67_RS04085 (DUT67_04085) pilT 905959..906975 (+) 1017 WP_048262112.1 type IV pilus twitching motility protein PilT Machinery gene
  DUT67_RS04090 (DUT67_04090) - 906944..908776 (-) 1833 WP_048262110.1 diguanylate cyclase -
  DUT67_RS04095 (DUT67_04095) - 909318..910889 (-) 1572 WP_048262108.1 methyl-accepting chemotaxis protein -
  DUT67_RS04100 (DUT67_04100) nsrR 911185..911610 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36704.97 Da        Isoelectric Point: 6.4228

>NTDB_id=1022664 DUT67_RS04085 WP_048262112.1 905959..906975(+) (pilT) [Pectobacterium peruviense strain A350-S18-N16]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTPEQVAQWCDAWLEPAQKEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDELHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMVGALNDNSDRHVITLE
DPIEFIHVSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLSGAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCVLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1022664 DUT67_RS04085 WP_048262112.1 905959..906975(+) (pilT) [Pectobacterium peruviense strain A350-S18-N16]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCTTCGGATCTGCACCTTTGTAGCGGGCATCCTCCGGT
GTTGCGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTAGAACCGGCTCAGAAGGAGCAACTGCGGCAGGCAGGGCAGGTGGACGGCGCGTTAATGCTGCCTGATGGACAG
CGCCTGCGGGTAAATGTCTTTCGCCAGAGAGAGGGGCTGTCTGCGGCCTTACGTATTATCCCGTCTGTTCAGCCTTCATT
GGATGAATTACATGCGCCACCCGTGCTCTCGACGCTGCTGGAGAAGCCAAACGGACTGATCCTGATTACCGGCGCGACGG
GCAGCGGTAAATCCACGACGTTGGCAGCGATGGTCGGGGCGTTAAACGACAACAGCGATCGCCATGTGATTACGTTGGAG
GATCCGATCGAGTTTATTCATGTCAGCCGACGTTGCTTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCTTCTTTTGC
GCAGGCGCTACGGGCGGCATTGAGGGAAGATCCTGATGTCATTCTGCTGGGAGAGTTGCGCGATACGGAGACGATTCGGC
TGGCGTTGACAGCCGCGGAAACTGGCCACTTGGTGCTATCAACGCTACATACGCGCAGTGCATCACAGGCGGTAGACCGT
TTGATTGATGTCTTTCCCGGTGAAGAAAAAGCCTATGTCCGTAGCCAGCTAGCGACCTGCTTGCAGGCGGTGGTGACGCA
AAAGCTGTTGTCTGGAGCGCAAGGCGGTCGAATCGCGCTTTATGAAGTGCTGACCGCCACCGCGGCAGTCAGCAACCTGA
TTCGTGAGGGAAAAACACATCAGCTTCCCGGCCTGATTCAGACCGGAGCCGCTGCGGGAATGCAAACATTTGAACAAAGC
TACCAGCAGCGCTGCCGGGATGGGCTTATTTCACACAGTTGCGTCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Acinetobacter baylyi ADP1

48.225

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.64

97.929

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.64

97.929

0.476
  pilT Acinetobacter nosocomialis M2

48.338

97.929

0.473
  pilT Legionella pneumophila strain Lp02

48.338

97.929

0.473
  pilT Legionella pneumophila strain ERS1305867

48.338

97.929

0.473
  pilT Acinetobacter baumannii D1279779

48.338

97.929

0.473
  pilT Acinetobacter baumannii strain A118

48.338

97.929

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.342

98.521

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.763

99.704

0.396
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364

Multiple sequence alignment