Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB1748_RS17060 Genome accession   NZ_CP160631
Coordinates   3636813..3637805 (-) Length   330 a.a.
NCBI ID   WP_367395816.1    Uniprot ID   -
Organism   Pantoea sp. Ep11b     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3631813..3642805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1748_RS17030 (AB1748_17030) - 3632590..3633102 (+) 513 WP_111140654.1 SprT family zinc-dependent metalloprotease -
  AB1748_RS17035 (AB1748_17035) endA 3633179..3633883 (+) 705 WP_111140655.1 deoxyribonuclease I -
  AB1748_RS17040 (AB1748_17040) rsmE 3633955..3634686 (+) 732 WP_367395815.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB1748_RS17045 (AB1748_17045) gshB 3634793..3635737 (+) 945 WP_293774585.1 glutathione synthase -
  AB1748_RS17050 (AB1748_17050) - 3635824..3636387 (+) 564 WP_111142281.1 YqgE/AlgH family protein -
  AB1748_RS17055 (AB1748_17055) ruvX 3636387..3636806 (+) 420 WP_111142280.1 Holliday junction resolvase RuvX -
  AB1748_RS17060 (AB1748_17060) pilT 3636813..3637805 (-) 993 WP_367395816.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1748_RS17065 (AB1748_17065) - 3637820..3638527 (+) 708 WP_293774579.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1748_RS17070 (AB1748_17070) - 3638540..3639094 (+) 555 WP_111140730.1 YggT family protein -
  AB1748_RS17075 (AB1748_17075) - 3639116..3639709 (+) 594 WP_111140731.1 XTP/dITP diphosphatase -
  AB1748_RS17080 (AB1748_17080) hemW 3639702..3640841 (+) 1140 WP_367395817.1 radical SAM family heme chaperone HemW -
  AB1748_RS17085 (AB1748_17085) - 3640878..3641594 (-) 717 WP_367395818.1 DUF2884 domain-containing protein -
  AB1748_RS17090 (AB1748_17090) - 3641699..3642025 (-) 327 WP_128086614.1 YggL family protein -
  AB1748_RS17095 (AB1748_17095) trmB 3642025..3642744 (-) 720 WP_367395819.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35612.12 Da        Isoelectric Point: 7.1858

>NTDB_id=1021719 AB1748_RS17060 WP_367395816.1 3636813..3637805(-) (pilT) [Pantoea sp. Ep11b]
MDLDEMVALSVKHNAADLHLCSGHLPCWRRQGRLETMPQQPRITTEGIAQFMAQWLSDSQRQQLAQAGQIDFALSMADGS
RLRANLFRQREGLSLALRIIATTCPTLAELQLPASVPPLLAQQDGLILVTGATGSGKSTTLAALVDALNRQPACHIITLE
DPIEFIHQSRQSLIQQREIGLHCASFALGIRAALREDPDVILLGELRDSDTIRQALTAAETGHLVLATLHTRGAAQAVDR
LVDVFPAEEKPLVRSQLAGSLKAVIAQRLVPSAAGGRIGLFEILIATPGITNLIREGKMHQIPALLQTGAQAGMQTFEQS
RLARQAAGLI

Nucleotide


Download         Length: 993 bp        

>NTDB_id=1021719 AB1748_RS17060 WP_367395816.1 3636813..3637805(-) (pilT) [Pantoea sp. Ep11b]
ATGGATCTGGATGAAATGGTGGCGCTTAGTGTAAAGCATAATGCCGCCGATCTGCACCTTTGCAGCGGACATTTGCCCTG
CTGGCGGCGGCAGGGAAGGCTGGAGACGATGCCGCAGCAGCCCCGCATCACCACGGAGGGCATCGCGCAGTTTATGGCAC
AGTGGCTCAGTGACTCACAGCGCCAGCAGCTGGCGCAGGCGGGCCAGATCGATTTTGCCCTGAGTATGGCGGACGGCTCA
CGGCTGCGTGCAAATCTCTTCAGGCAGCGCGAGGGTCTGTCGCTGGCGCTGCGGATTATCGCCACGACATGCCCGACACT
GGCAGAACTGCAACTGCCCGCCAGCGTACCCCCACTGCTGGCGCAGCAGGATGGGCTGATTCTGGTGACCGGTGCGACGG
GCAGCGGCAAATCCACCACGCTGGCGGCGCTGGTGGATGCGCTGAACCGCCAGCCGGCGTGTCATATCATCACGCTGGAA
GATCCGATTGAGTTTATCCATCAGAGCCGGCAGTCGCTGATTCAGCAGCGGGAGATCGGTCTGCACTGCGCGTCGTTCGC
GCTGGGCATCAGGGCGGCACTGCGTGAAGACCCGGACGTTATCCTGCTGGGTGAGCTTCGCGACAGCGACACCATTCGTC
AGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGCTGGCAACGCTGCATACCCGGGGGGCGGCGCAGGCGGTGGATCGG
CTGGTGGATGTCTTTCCGGCGGAGGAGAAGCCGCTGGTGCGGAGCCAGCTGGCGGGCAGCCTGAAGGCGGTGATCGCGCA
GCGTCTGGTGCCGTCAGCGGCGGGCGGGCGCATTGGACTGTTTGAGATACTGATCGCCACGCCCGGCATCACCAATCTGA
TCCGCGAGGGGAAGATGCACCAGATCCCCGCGCTGTTGCAGACCGGCGCGCAGGCCGGTATGCAGACGTTTGAGCAGAGC
CGTCTGGCGCGGCAGGCCGCCGGTCTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.649

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.348

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.438

96.97

0.47
  pilT Vibrio cholerae strain A1552

48.438

96.97

0.47
  pilT Acinetobacter baylyi ADP1

45.758

100

0.458
  pilT Pseudomonas stutzeri DSM 10701

45.758

100

0.458
  pilT Acinetobacter baumannii D1279779

45.455

100

0.455
  pilT Acinetobacter baumannii strain A118

45.455

100

0.455
  pilT Pseudomonas aeruginosa PAK

45.455

100

0.455
  pilT Acinetobacter nosocomialis M2

45.152

100

0.452
  pilT Legionella pneumophila strain ERS1305867

45.455

96.667

0.439
  pilT Legionella pneumophila strain Lp02

45.455

96.667

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.574

96.667

0.421
  pilU Pseudomonas stutzeri DSM 10701

39.198

98.182

0.385
  pilU Vibrio cholerae strain A1552

38.006

97.273

0.37
  pilU Acinetobacter baylyi ADP1

37.578

97.576

0.367

Multiple sequence alignment