Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABV589_RS12895 Genome accession   NZ_CP160398
Coordinates   2860584..2861618 (-) Length   344 a.a.
NCBI ID   WP_027610581.1    Uniprot ID   A0A854X3V5
Organism   Pseudomonas sp. HOU2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2855584..2866618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV589_RS12870 (ABV589_12870) - 2856057..2857328 (+) 1272 WP_367086072.1 dihydroorotase -
  ABV589_RS12875 (ABV589_12875) - 2857651..2858637 (+) 987 WP_367086073.1 lipid II-degrading bacteriocin -
  ABV589_RS12880 (ABV589_12880) - 2858678..2859082 (+) 405 WP_367086074.1 hypothetical protein -
  ABV589_RS12885 (ABV589_12885) - 2859156..2859587 (-) 432 WP_047596579.1 TM2 domain-containing protein -
  ABV589_RS12890 (ABV589_12890) - 2859836..2860462 (+) 627 WP_108591856.1 C40 family peptidase -
  ABV589_RS12895 (ABV589_12895) pilT 2860584..2861618 (-) 1035 WP_027610581.1 type IV pilus twitching motility protein PilT Machinery gene
  ABV589_RS12900 (ABV589_12900) - 2861675..2862361 (+) 687 WP_367086075.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABV589_RS12905 (ABV589_12905) proC 2862390..2863208 (+) 819 WP_367086076.1 pyrroline-5-carboxylate reductase -
  ABV589_RS12910 (ABV589_12910) - 2863219..2863809 (+) 591 WP_367086077.1 YggT family protein -
  ABV589_RS12915 (ABV589_12915) - 2863806..2864102 (+) 297 WP_115985948.1 DUF167 domain-containing protein -
  ABV589_RS12920 (ABV589_12920) - 2864332..2865471 (+) 1140 WP_258676957.1 homoserine O-acetyltransferase -
  ABV589_RS12925 (ABV589_12925) metW 2865479..2866099 (+) 621 WP_367086078.1 methionine biosynthesis protein MetW -
  ABV589_RS12930 (ABV589_12930) - 2866126..2866560 (+) 435 WP_329697136.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38083.79 Da        Isoelectric Point: 7.0158

>NTDB_id=1020864 ABV589_RS12895 WP_027610581.1 2860584..2861618(-) (pilT) [Pseudomonas sp. HOU2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNIHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KELVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1020864 ABV589_RS12895 WP_027610581.1 2860584..2861618(-) (pilT) [Pseudomonas sp. HOU2]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCGGGGCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGCGTCGACTTCGAGAAACACCTGGAAACGGATTTCTCGTTCGAAGTCCCCGGCGTGGCGCGC
TTCCGGGTCAATGCGTTCAACCAGAACCGGGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGAAAGTGCTTAGCATGGA
AGACCTCGGCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGCAAATCGACCACGCTGGCGGCGATGATCGATTACCTCAACATCCATCGCCATCACCACATTCTCACCATCGAAGAC
CCGATCGAGTTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGCTTCGCCAC
CGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACGTAATTCTGGTGGGCGAGATGCGTGATCTGGAAACCATCCGTCTGG
CGCTGACCGCTGCCGAGACCGGGCATCTGGTGTTCGGCACGCTGCACACCACATCGGCGGCGAAAACCATCGACCGTGTT
GTCGATGTGTTCCCGGGTGACGAGAAGTCCATGGTGCGTTCGATGTTGTCCGAGTCGCTGCTGGCAGTGGTATCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGGCGGGTGGCAGCCCACGAGATCATGCTGGGAACGTCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAAATGTATTCGGCGATTCAGACCGGTGGGTCGTTGGGTATGCAGACACTCGACATGTGCCTG
AAGGAGTTGGTGACCAAAGGCTTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854X3V5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment