Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAFM42_RS08110 Genome accession   NZ_CP160329
Coordinates   1574269..1575303 (-) Length   344 a.a.
NCBI ID   WP_053258478.1    Uniprot ID   A0A1E4XEA2
Organism   Pseudomonas fluorescens strain PFX1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1569269..1580303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFM42_RS08075 (AAFM42_08030) - 1569308..1569877 (+) 570 WP_053258472.1 YqgE/AlgH family protein -
  AAFM42_RS08080 (AAFM42_08035) ruvX 1569877..1570314 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  AAFM42_RS08085 (AAFM42_08040) pyrR 1570315..1570821 (+) 507 WP_053258473.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AAFM42_RS08090 (AAFM42_08045) - 1570835..1571839 (+) 1005 WP_053258474.1 aspartate carbamoyltransferase catalytic subunit -
  AAFM42_RS08095 (AAFM42_08050) - 1571836..1573107 (+) 1272 WP_053258475.1 dihydroorotase -
  AAFM42_RS08100 (AAFM42_08055) - 1573131..1573559 (-) 429 WP_053258476.1 NINE protein -
  AAFM42_RS08105 (AAFM42_08060) - 1573612..1574247 (+) 636 WP_053258477.1 C40 family peptidase -
  AAFM42_RS08110 (AAFM42_08065) pilT 1574269..1575303 (-) 1035 WP_053258478.1 type IV pilus twitching motility protein PilT Machinery gene
  AAFM42_RS08115 (AAFM42_08070) - 1575318..1576013 (+) 696 WP_053258479.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAFM42_RS08120 (AAFM42_08075) proC 1576039..1576857 (+) 819 WP_053258480.1 pyrroline-5-carboxylate reductase -
  AAFM42_RS08125 (AAFM42_08080) - 1576904..1577491 (+) 588 WP_053258481.1 YggT family protein -
  AAFM42_RS08130 (AAFM42_08085) - 1577541..1578680 (+) 1140 WP_053258482.1 homoserine O-acetyltransferase -
  AAFM42_RS08135 (AAFM42_08090) metW 1578683..1579303 (+) 621 WP_003213768.1 methionine biosynthesis protein MetW -
  AAFM42_RS08140 (AAFM42_08095) - 1579326..1579760 (+) 435 WP_053258483.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37418.10 Da        Isoelectric Point: 6.7811

>NTDB_id=1020510 AAFM42_RS08110 WP_053258478.1 1574269..1575303(-) (pilT) [Pseudomonas fluorescens strain PFX1]
MDITELLTASVRRGASDLHLSAGVAPMLRVDGEVWPLDGPALSPPQVADLLSPLLNQYQEKDFETSLETDFAFELPGVAR
FRANVFQQARGMGAVFRTIATEVQSLESLGLGEVFQRITRFPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKMALINQRQVQRDTHSFSTALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTVHTTSAAKTVDRL
VDVFPAGEKAMVRTMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVNEGLISREEARGQARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1020510 AAFM42_RS08110 WP_053258478.1 1574269..1575303(-) (pilT) [Pseudomonas fluorescens strain PFX1]
ATGGATATCACTGAATTGCTGACGGCCAGCGTGCGTCGTGGCGCCTCTGACCTGCATTTGTCGGCTGGCGTGGCGCCGAT
GCTGCGTGTGGATGGCGAGGTCTGGCCCCTGGACGGCCCGGCGCTGTCGCCCCCGCAAGTGGCGGATTTATTGAGCCCTT
TGCTCAACCAGTACCAAGAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGCTGCCGGGCGTGGCGCGG
TTCCGGGCGAATGTGTTCCAGCAGGCGCGCGGCATGGGCGCGGTGTTTCGCACCATTGCGACCGAGGTGCAAAGCCTGGA
AAGCCTCGGGCTGGGAGAAGTGTTCCAACGGATCACCCGGTTTCCGCGCGGCCTGGTGTTGGTGACCGGGCCAACCGGGT
CCGGCAAGTCCACGACGCTGGCAGCGATGATCGACCATCTTAATCAGCACCGGCGCCAGCACATTCTTACGCTGGAAGAC
CCCATCGAATTTATCCACAGGCCGAAAATGGCCTTGATCAACCAGCGGCAGGTACAACGCGATACACACAGTTTTTCCAC
GGCGCTGCGCTCGGCGCTACGGGAAGACCCGGATGTCATTCTGGTGGGGGAGTTGCGTGACCTGGAAACCATACGGCTGG
CGCTGACGGCGGCTGAGACCGGGCACCTGGTGTTTGGCACCGTGCACACCACGTCGGCGGCAAAGACCGTGGACAGGCTG
GTGGATGTGTTCCCGGCCGGGGAAAAGGCCATGGTTCGCACGATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAGGT
GCTGGTCAAGAAGATCGGCGGCGGGCGGGTGGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATCCGTAACTTGATCC
GTGAAGACAAGGTGGCGCAAATGGTCTCCGCGATACAGACCGGTGGGGCGTTGGGCATGAAAACGTTGGATATGAGCTTG
AAGGCGTTGGTCAATGAGGGGTTGATCAGCCGGGAGGAAGCGCGGGGGCAGGCGCGGGTGCCGGGGGATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(84-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E4XEA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.727

99.128

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.968

99.128

0.703
  pilT Acinetobacter nosocomialis M2

69.501

99.128

0.689
  pilT Acinetobacter baumannii D1279779

69.501

99.128

0.689
  pilT Acinetobacter baumannii strain A118

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

69.208

99.128

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.798

97.965

0.625
  pilT Vibrio cholerae strain A1552

63.798

97.965

0.625
  pilT Legionella pneumophila strain Lp02

63.018

98.256

0.619
  pilT Legionella pneumophila strain ERS1305867

63.018

98.256

0.619
  pilT Neisseria meningitidis 8013

60.882

98.837

0.602
  pilT Neisseria gonorrhoeae MS11

60.588

98.837

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.254

97.384

0.48
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Vibrio cholerae strain A1552

39.13

100

0.392

Multiple sequence alignment