Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABU615_RS07915 Genome accession   NZ_CP160328
Coordinates   1770647..1771690 (+) Length   347 a.a.
NCBI ID   WP_267392020.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer08     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1765647..1776690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU615_RS07900 (ABU615_15265) - 1766513..1768186 (+) 1674 WP_370388794.1 von Willebrand factor type A domain-containing protein -
  ABU615_RS07905 (ABU615_04085) pdxY 1768813..1769679 (+) 867 WP_370388795.1 pyridoxal kinase PdxY -
  ABU615_RS07910 (ABU615_04080) - 1769771..1770487 (-) 717 WP_267409006.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABU615_RS07915 (ABU615_04075) pilT 1770647..1771690 (+) 1044 WP_267392020.1 type IV pilus twitching motility protein PilT Machinery gene
  ABU615_RS07920 (ABU615_04065) - 1772078..1773286 (+) 1209 WP_370387142.1 PilT/PilU family type 4a pilus ATPase -
  ABU615_RS07925 (ABU615_04060) murU 1773392..1774096 (+) 705 WP_370388796.1 N-acetylmuramate alpha-1-phosphate uridylyltransferase MurU -
  ABU615_RS07930 (ABU615_04055) ruvC 1774217..1774768 (+) 552 WP_267392024.1 crossover junction endodeoxyribonuclease RuvC -
  ABU615_RS07935 (ABU615_04050) - 1774878..1775663 (-) 786 WP_370388797.1 DUF2071 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38166.79 Da        Isoelectric Point: 6.9838

>NTDB_id=1020497 ABU615_RS07915 WP_267392020.1 1770647..1771690(+) (pilT) [Snodgrassella alvi strain Acer08]
MQLTDLLAFGVKNKASDLHLSAGLPPMIRVNGDIRRINLPEQSAEEVGKMIASIMNDYQRKDYQQNLETDFSFELPNVSR
FRVNAFNTERGPAAVMRTIPSKVLTLEELKAPAIFQKIAEMPRGLVLVTGPTGSGKSTTLAAMIDYINNHDAGHILTIED
PIEFVHQSKKSLVNQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLQAVISQTLLKTKDGNGRVAAHEIMISTPAVRNLIRENKIAQINSTLQTGQKFGMQTLDQC
LQQLLRENRISLDVARSKAVSPDQLAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1020497 ABU615_RS07915 WP_267392020.1 1770647..1771690(+) (pilT) [Snodgrassella alvi strain Acer08]
ATGCAGCTAACCGATTTATTGGCCTTCGGCGTTAAAAACAAAGCTTCCGACTTGCATCTGAGTGCTGGTCTTCCTCCTAT
GATACGCGTTAATGGTGATATCCGCCGTATTAATCTGCCCGAACAGAGCGCAGAAGAAGTAGGTAAAATGATAGCTTCCA
TTATGAACGACTATCAACGTAAAGATTATCAGCAAAATCTGGAAACTGACTTTTCGTTCGAACTTCCCAATGTCTCTCGG
TTTCGTGTCAATGCCTTTAACACCGAGCGCGGCCCAGCTGCAGTTATGCGTACCATTCCCAGTAAGGTACTGACGCTGGA
AGAGCTCAAAGCGCCAGCAATTTTTCAAAAAATTGCTGAAATGCCACGTGGACTAGTGCTGGTAACAGGACCTACCGGCT
CCGGTAAATCAACCACTTTGGCTGCAATGATTGATTACATCAATAATCACGATGCCGGCCATATTCTAACCATTGAAGAT
CCGATTGAGTTTGTGCATCAGAGTAAAAAATCGTTGGTAAACCAGCGTGAACTTCATCAGCATACACTCAGCTTTGCTAA
TGCACTGCGTTCAGCTTTGCGCGAAGACCCTGACGTGATTCTGGTAGGGGAAATGCGAGACCCTGAAACAATAGGTCTGG
CACTGACAGCTGCTGAAACCGGTCACCTTGTGTTCGGTACTTTGCATACTACTGGTGCAGCAAAAACGGTAGACCGTATT
GTGGACGTATTTCCTGCTGGTGAAAAAGAAATGGTGCGTTCTATGTTGTCTGAAAGTCTTCAGGCAGTAATTTCTCAGAC
TTTACTGAAAACTAAGGATGGCAATGGCCGTGTTGCAGCTCATGAAATCATGATCAGTACTCCGGCAGTGCGCAACCTGA
TTCGTGAGAACAAAATTGCTCAGATTAACTCTACTTTGCAGACGGGGCAGAAATTTGGTATGCAAACGTTAGACCAGTGT
TTACAACAATTGCTGCGTGAAAATCGTATCAGTTTAGATGTAGCACGATCTAAAGCGGTCAGTCCTGATCAGCTGGCAGG
ATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

82.37

99.712

0.821
  pilT Neisseria gonorrhoeae MS11

82.081

99.712

0.818
  pilT Pseudomonas stutzeri DSM 10701

71.884

99.424

0.715
  pilT Acinetobacter baylyi ADP1

71.304

99.424

0.709
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter nosocomialis M2

69.855

99.424

0.695
  pilT Acinetobacter baumannii D1279779

69.855

99.424

0.695
  pilT Acinetobacter baumannii strain A118

69.855

99.424

0.695
  pilT Legionella pneumophila strain Lp02

68.035

98.271

0.669
  pilT Legionella pneumophila strain ERS1305867

68.035

98.271

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.497

97.406

0.628
  pilT Vibrio cholerae strain A1552

64.497

97.406

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.023

99.712

0.519
  pilU Vibrio cholerae strain A1552

41.739

99.424

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.449

99.424

0.412
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406

Multiple sequence alignment