Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABW387_RS09070 Genome accession   NZ_CP159911
Coordinates   2075019..2076062 (+) Length   347 a.a.
NCBI ID   WP_084552601.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Amel02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2070019..2081062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABW387_RS09055 (ABW387_18340) - 2070819..2072552 (+) 1734 WP_370385774.1 von Willebrand factor type A domain-containing protein -
  ABW387_RS09060 (ABW387_01000) pdxY 2073197..2074063 (+) 867 WP_037392375.1 pyridoxal kinase PdxY -
  ABW387_RS09065 (ABW387_00995) - 2074140..2074859 (-) 720 WP_370385775.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABW387_RS09070 (ABW387_00990) pilT 2075019..2076062 (+) 1044 WP_084552601.1 type IV pilus twitching motility protein PilT Machinery gene
  ABW387_RS09075 (ABW387_00985) - 2076464..2077693 (+) 1230 WP_370386273.1 PilT/PilU family type 4a pilus ATPase -
  ABW387_RS09080 (ABW387_00980) murU 2077825..2078526 (+) 702 WP_370385776.1 N-acetylmuramate alpha-1-phosphate uridylyltransferase MurU -
  ABW387_RS09085 (ABW387_00975) ruvC 2078657..2079208 (+) 552 WP_370385777.1 crossover junction endodeoxyribonuclease RuvC -
  ABW387_RS09090 (ABW387_00970) - 2079306..2080091 (-) 786 WP_367465514.1 DUF2071 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38205.83 Da        Isoelectric Point: 6.9840

>NTDB_id=1016699 ABW387_RS09070 WP_084552601.1 2075019..2076062(+) (pilT) [Snodgrassella alvi strain Amel02]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVNGDIRRINLPELSAEEVGKMIASIMNDYQRKEYQQNLETDFSFELPNVSR
FRVNAFNTERGPAAVMRTIPSKVLTLEELRAPAIFQKIAELPRGLVLVTGPTGSGKSTTLAAMIDYINNNDAGHILTIED
PIEFVHQSKKSLVNQRELHQHTHSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLQAVISQTLLKTKDGNGRVAAHEIMISNPAVRNLIRENKIAQINSTLQTGQKFGMQTLDQC
LQQLVRENRISIDVARAKAVSPDQMVG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1016699 ABW387_RS09070 WP_084552601.1 2075019..2076062(+) (pilT) [Snodgrassella alvi strain Amel02]
ATGCAGATAACCGATTTATTGGCCTTCGGCGTTAAAAATAAAGCTTCCGACTTGCATCTGAGTGCTGGTCTGCCTCCCAT
GATACGCGTTAATGGTGATATCCGCCGTATCAATCTGCCTGAACTGAGTGCAGAAGAAGTAGGCAAAATGATTGCTTCTA
TCATGAACGATTATCAGCGCAAGGAATATCAGCAAAACCTAGAAACTGACTTTTCGTTCGAATTGCCCAATGTATCGCGG
TTCCGTGTGAATGCCTTTAATACTGAACGCGGTCCTGCAGCCGTTATGCGTACTATTCCGAGTAAAGTTCTTACACTTGA
AGAACTGAGGGCTCCGGCAATTTTTCAGAAAATTGCAGAACTGCCGCGCGGGCTGGTACTGGTAACCGGTCCTACTGGTT
CCGGTAAGTCAACTACACTGGCAGCAATGATCGACTACATCAATAATAATGATGCCGGTCATATTCTAACAATTGAAGAC
CCGATTGAGTTTGTACATCAAAGTAAAAAATCGTTGGTAAACCAGCGTGAACTGCATCAGCATACTCATAGTTTCGCCAA
TGCCCTGCGTTCGGCACTGCGTGAAGATCCAGATGTGATTCTGGTAGGCGAAATGCGTGACCCTGAAACGATTGGTTTGG
CCTTAACTGCTGCCGAAACAGGTCACTTGGTGTTTGGTACTTTGCATACCACAGGTGCTGCTAAAACCGTTGACCGTATT
GTGGATGTATTCCCTGCAGGCGAAAAAGAAATGGTTCGTTCTATGCTGTCAGAAAGTTTGCAGGCAGTGATTTCTCAGAC
TCTGCTTAAAACCAAAGATGGTAACGGCCGTGTGGCAGCACATGAAATTATGATCAGTAATCCGGCAGTACGTAACCTGA
TTCGGGAAAACAAAATTGCTCAGATCAACTCTACTTTACAAACTGGTCAGAAATTCGGAATGCAGACACTCGATCAGTGT
TTACAGCAATTAGTGCGTGAAAACCGTATTAGTATAGATGTTGCACGTGCCAAAGCAGTAAGTCCGGATCAGATGGTAGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

83.188

99.424

0.827
  pilT Neisseria gonorrhoeae MS11

82.899

99.424

0.824
  pilT Acinetobacter nosocomialis M2

70.809

99.712

0.706
  pilT Acinetobacter baumannii D1279779

70.809

99.712

0.706
  pilT Acinetobacter baumannii strain A118

70.809

99.712

0.706
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

70.435

99.424

0.7
  pilT Legionella pneumophila strain Lp02

68.622

98.271

0.674
  pilT Legionella pneumophila strain ERS1305867

68.622

98.271

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.769

93.66

0.625
  pilT Vibrio cholerae strain A1552

66.769

93.66

0.625
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.424

0.409
  pilU Vibrio cholerae strain A1552

42.296

95.389

0.403
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403

Multiple sequence alignment