Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNK30_RS17975 Genome accession   NZ_CP159509
Coordinates   3902356..3903483 (+) Length   375 a.a.
NCBI ID   WP_004141526.1    Uniprot ID   A0A854E1G1
Organism   Stenotrophomonas maltophilia strain SM911     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3883812..3909653 3902356..3903483 within 0


Gene organization within MGE regions


Location: 3883812..3909653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNK30_RS17895 - 3883812..3885719 (-) 1908 WP_111188254.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  ABNK30_RS17900 - 3885716..3886450 (-) 735 WP_049402806.1 NTP transferase domain-containing protein -
  ABNK30_RS17905 - 3886458..3887252 (-) 795 WP_033835588.1 glycosyltransferase family 2 protein -
  ABNK30_RS17910 - 3887242..3887676 (-) 435 WP_033835587.1 GtrA family protein -
  ABNK30_RS17915 - 3887676..3889538 (-) 1863 WP_049402807.1 hypothetical protein -
  ABNK30_RS17920 - 3889717..3890085 (-) 369 WP_051188019.1 hypothetical protein -
  ABNK30_RS17925 - 3890139..3891431 (-) 1293 WP_033835934.1 glycosyltransferase family 4 protein -
  ABNK30_RS17930 - 3891428..3894592 (-) 3165 WP_053091559.1 class I SAM-dependent methyltransferase -
  ABNK30_RS17935 - 3894589..3895167 (-) 579 WP_033835933.1 CatB-related O-acetyltransferase -
  ABNK30_RS17940 - 3895164..3896567 (-) 1404 WP_049397550.1 ABC transporter ATP-binding protein -
  ABNK30_RS17945 - 3896557..3897387 (-) 831 WP_080240930.1 ABC transporter permease -
  ABNK30_RS17950 - 3897460..3898632 (-) 1173 WP_111188255.1 cystathionine gamma-synthase -
  ABNK30_RS17955 - 3898756..3900126 (-) 1371 WP_005407965.1 pyridoxal-phosphate dependent enzyme -
  ABNK30_RS17960 - 3900427..3900663 (-) 237 WP_005407964.1 YdcH family protein -
  ABNK30_RS17965 - 3900988..3901872 (-) 885 WP_080240928.1 hypothetical protein -
  ABNK30_RS17970 - 3901869..3902234 (-) 366 WP_005407961.1 DUF4398 domain-containing protein -
  ABNK30_RS17975 pilU 3902356..3903483 (+) 1128 WP_004141526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNK30_RS17980 - 3903614..3904897 (+) 1284 WP_095378724.1 putative DNA modification/repair radical SAM protein -
  ABNK30_RS17985 - 3904878..3906338 (+) 1461 WP_111188256.1 UdgX family uracil-DNA binding protein -
  ABNK30_RS17990 maiA 3906384..3907052 (-) 669 WP_111188257.1 maleylacetoacetate isomerase -
  ABNK30_RS17995 - 3907067..3908056 (-) 990 WP_049430901.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41398.70 Da        Isoelectric Point: 6.3741

>NTDB_id=1013937 ABNK30_RS17975 WP_004141526.1 3902356..3903483(+) (pilU) [Stenotrophomonas maltophilia strain SM911]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLLNTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1013937 ABNK30_RS17975 WP_004141526.1 3902356..3903483(+) (pilU) [Stenotrophomonas maltophilia strain SM911]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGATGCCGGTCGC
TTCCGCGTCAATGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGTGCCATCCGCAGCCGGATTCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTACTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAAGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAAAACGCGATGCGTGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCAGAGACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAAGTACTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCATCGCTGGAAGAGGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAACAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCATGACCCGTACGCGGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854E1G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

95.733

0.488
  pilU Vibrio cholerae strain A1552

49.435

94.4

0.467
  pilU Acinetobacter baylyi ADP1

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

41.141

88.8

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.8

0.365
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36

Multiple sequence alignment