Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABN702_RS03550 Genome accession   NZ_CP158164
Coordinates   747465..748505 (+) Length   346 a.a.
NCBI ID   WP_417899421.1    Uniprot ID   -
Organism   Bacillus sp. CSS-39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742465..753505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABN702_RS03525 (ABN702_03505) - 742469..743125 (-) 657 WP_417899416.1 PulJ/GspJ family protein -
  ABN702_RS03530 (ABN702_03510) - 743109..743807 (-) 699 WP_417899417.1 type IV pilus modification PilV family protein -
  ABN702_RS03535 (ABN702_03515) - 743910..744299 (+) 390 WP_417899418.1 hypothetical protein -
  ABN702_RS03540 (ABN702_03520) - 744325..745764 (+) 1440 WP_417899419.1 hypothetical protein -
  ABN702_RS03545 (ABN702_03525) - 745785..747452 (+) 1668 WP_417899420.1 GspE/PulE family protein -
  ABN702_RS03550 (ABN702_03530) pilT 747465..748505 (+) 1041 WP_417899421.1 type IV pilus twitching motility protein PilT Machinery gene
  ABN702_RS03555 (ABN702_03535) - 748506..749714 (+) 1209 WP_417899422.1 type II secretion system F family protein -
  ABN702_RS03560 (ABN702_03540) - 749811..750227 (+) 417 WP_417899423.1 type IV pilin protein -
  ABN702_RS03565 (ABN702_03545) - 750231..750977 (+) 747 WP_417899424.1 prepilin peptidase -
  ABN702_RS03570 (ABN702_03550) pilM 751002..751982 (+) 981 WP_417899425.1 type IV pilus biogenesis protein PilM -
  ABN702_RS03575 (ABN702_03555) - 751985..752659 (+) 675 WP_417899426.1 PilN domain-containing protein -
  ABN702_RS03580 (ABN702_03560) - 752656..753381 (+) 726 WP_417899427.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38794.59 Da        Isoelectric Point: 6.8379

>NTDB_id=1010018 ABN702_RS03550 WP_417899421.1 747465..748505(+) (pilT) [Bacillus sp. CSS-39]
MLDKMNDWLHAAYEYKASDIHLSVGVPPVFRINGELKSYGKDILTPEDTHTIAKQMIPEFMWQTFDEKGELDFSYSVPGI
SRFRVNAYRQRTSVAIAIRIIPSRVPSLEELSLPPILNSLVDKPQGLILVTGPTGSGKSTTLASMIDYMNRTMKKHIITL
EDPIEYLHRHNQCMIDQREVGFDTKSFATGLRASLRQDPDVILVGELRDLETIQTAITAAETGHLVLGTLHTTSAPSTID
RIIDVFPGPQQAQIRIQLASVLVSIISQRLFQSLDRQGRKVATEILVNTPAVANLIRNEKIHQIQNVMQTSRALGMHTLE
MSIRQLIQNSEISRVSAESFLQETVN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1010018 ABN702_RS03550 WP_417899421.1 747465..748505(+) (pilT) [Bacillus sp. CSS-39]
ATGCTGGATAAAATGAACGACTGGCTCCATGCAGCCTATGAATATAAAGCTTCGGATATCCATCTGTCCGTTGGTGTACC
ACCGGTTTTTCGCATAAACGGGGAGCTCAAAAGCTATGGAAAAGATATTCTCACCCCGGAGGATACACATACGATTGCCA
AACAGATGATACCTGAATTTATGTGGCAGACATTTGATGAAAAAGGGGAACTTGACTTTTCATACAGTGTCCCCGGGATA
TCAAGGTTCCGTGTGAACGCCTATCGCCAAAGAACATCCGTTGCAATTGCCATACGTATCATCCCTAGCCGGGTACCATC
TCTTGAAGAGCTTTCCTTGCCGCCTATTTTGAATAGTCTTGTAGATAAGCCACAGGGACTGATTCTGGTTACCGGGCCTA
CCGGAAGCGGAAAGTCGACAACACTTGCTTCCATGATTGATTACATGAACCGCACAATGAAGAAGCACATCATTACCTTG
GAAGATCCAATCGAGTACTTACATCGCCATAATCAGTGCATGATCGATCAACGTGAGGTCGGATTTGATACAAAGAGCTT
TGCAACAGGCCTGAGAGCATCTCTAAGACAGGATCCGGATGTTATTCTTGTAGGGGAACTTCGTGACCTGGAGACCATCC
AGACTGCTATCACTGCAGCAGAAACCGGACATCTTGTATTGGGAACACTGCATACTACCAGCGCCCCATCCACCATTGAC
CGGATCATTGACGTATTTCCTGGACCACAGCAGGCGCAAATTCGTATACAGCTTGCTTCGGTGCTCGTATCCATCATCTC
CCAAAGGCTTTTCCAGTCATTGGACAGACAAGGCAGGAAAGTGGCGACGGAAATCCTGGTCAACACACCGGCAGTTGCCA
ACCTGATTCGGAACGAGAAAATACATCAGATTCAAAACGTGATGCAAACTAGCAGAGCCCTCGGGATGCACACCTTGGAA
ATGAGCATCAGGCAGCTGATTCAAAATAGTGAAATCAGCAGAGTATCAGCAGAAAGCTTTCTGCAGGAGACGGTGAATTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.16

93.642

0.488
  pilT Acinetobacter nosocomialis M2

48.193

95.954

0.462
  pilT Acinetobacter baumannii D1279779

48.193

95.954

0.462
  pilT Acinetobacter baumannii strain A118

48.193

95.954

0.462
  pilT Vibrio cholerae strain A1552

49.533

92.775

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.533

92.775

0.46
  pilT Pseudomonas aeruginosa PAK

47.748

96.243

0.46
  pilT Acinetobacter baylyi ADP1

46.866

96.821

0.454
  pilT Neisseria meningitidis 8013

45.882

98.266

0.451
  pilT Pseudomonas stutzeri DSM 10701

46.407

96.532

0.448
  pilT Legionella pneumophila strain ERS1305867

46.224

95.665

0.442
  pilT Legionella pneumophila strain Lp02

46.224

95.665

0.442
  pilT Neisseria gonorrhoeae MS11

46.364

95.376

0.442
  pilU Acinetobacter baylyi ADP1

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

41.875

92.486

0.387
  pilU Vibrio cholerae strain A1552

39.82

96.532

0.384

Multiple sequence alignment