Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABNP41_RS20075 Genome accession   NZ_CP157869
Coordinates   4215696..4216718 (-) Length   340 a.a.
NCBI ID   WP_049300548.1    Uniprot ID   -
Organism   Serratia marcescens strain KS10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4210696..4221718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP41_RS20045 (ABNP41_20035) - 4211496..4212008 (+) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -
  ABNP41_RS20050 (ABNP41_20040) endA 4212110..4212805 (+) 696 WP_049300546.1 deoxyribonuclease I -
  ABNP41_RS20055 (ABNP41_20045) rsmE 4212875..4213606 (+) 732 WP_049300547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ABNP41_RS20060 (ABNP41_20050) gshB 4213617..4214567 (+) 951 WP_016930084.1 glutathione synthase -
  ABNP41_RS20065 (ABNP41_20055) - 4214714..4215277 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  ABNP41_RS20070 (ABNP41_20060) ruvX 4215277..4215699 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  ABNP41_RS20075 (ABNP41_20065) pilT 4215696..4216718 (-) 1023 WP_049300548.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNP41_RS20080 (ABNP41_20070) - 4216739..4217446 (+) 708 WP_049300549.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNP41_RS20085 (ABNP41_20075) proC 4217466..4218287 (+) 822 WP_033639391.1 pyrroline-5-carboxylate reductase -
  ABNP41_RS20090 (ABNP41_20080) - 4218319..4218873 (+) 555 WP_004937436.1 YggT family protein -
  ABNP41_RS20095 (ABNP41_20085) yggU 4218870..4219163 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  ABNP41_RS20100 (ABNP41_20090) - 4219304..4219897 (+) 594 WP_350024338.1 XTP/dITP diphosphatase -
  ABNP41_RS20105 (ABNP41_20095) hemW 4219890..4221032 (+) 1143 WP_044030492.1 radical SAM family heme chaperone HemW -
  ABNP41_RS20110 (ABNP41_20100) - 4221070..4221504 (-) 435 WP_277496611.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36405.64 Da        Isoelectric Point: 7.0670

>NTDB_id=1008620 ABNP41_RS20075 WP_049300548.1 4215696..4216718(-) (pilT) [Serratia marcescens strain KS10]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAAQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1008620 ABNP41_RS20075 WP_049300548.1 4215696..4216718(-) (pilT) [Serratia marcescens strain KS10]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGCGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATTGTCCCGGCGTTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGTTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCCGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

39.815

95.294

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment