Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABL848_RS07345 Genome accession   NZ_CP157611
Coordinates   1578124..1579167 (+) Length   347 a.a.
NCBI ID   WP_093206426.1    Uniprot ID   A0A7Y7IBT6
Organism   Variovorax sp. NFACC28     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1573124..1584167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL848_RS07315 (ABL848_07320) - 1573614..1574276 (-) 663 WP_093306956.1 nucleoside/nucleotide kinase family protein -
  ABL848_RS07320 (ABL848_07325) - 1574313..1575587 (-) 1275 WP_093206430.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL848_RS07325 (ABL848_07330) - 1575820..1576146 (+) 327 WP_093206429.1 DUF4148 domain-containing protein -
  ABL848_RS07330 (ABL848_07335) - 1576282..1576746 (+) 465 WP_093306955.1 hypothetical protein -
  ABL848_RS07335 (ABL848_07340) - 1576885..1577352 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL848_RS07340 (ABL848_07345) - 1577368..1578072 (-) 705 WP_093306954.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL848_RS07345 (ABL848_07350) pilT 1578124..1579167 (+) 1044 WP_093206426.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL848_RS07350 (ABL848_07355) pilU 1579304..1580440 (+) 1137 WP_093206425.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL848_RS07355 (ABL848_07360) - 1580446..1581999 (-) 1554 WP_093306953.1 MFS transporter -
  ABL848_RS07360 (ABL848_07365) - 1581996..1582478 (-) 483 WP_093306952.1 MerR family transcriptional regulator -
  ABL848_RS07365 (ABL848_07370) - 1582566..1583474 (+) 909 WP_093306951.1 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.83 Da        Isoelectric Point: 6.8356

>NTDB_id=1007671 ABL848_RS07345 WP_093206426.1 1578124..1579167(+) (pilT) [Variovorax sp. NFACC28]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1007671 ABL848_RS07345 WP_093206426.1 1578124..1579167(+) (pilT) [Variovorax sp. NFACC28]
GTGGACATCACCCAATTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGATCTGCACCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAACGTCGACGCGCTCGACCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCCGTGTTCCGGACCATTCCATCGAAGATCCTCACGCTCGA
GCAGCTCAATGCGCCGAAGATTTTCGGCGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACAGTGGAGGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTCTCCAA
CGCCCTACGCTCCGCGCTGCGCGAAGACCCCGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCCGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGGCGAAGAGAAGGAAATGATCCGCGCGATGCTCTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGATGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGGAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACGGAACTGGTGCGCCGCAATACCATCTCGGCAGCAGAGGCCCGCGGCAAGGCCAAGATCCCAGAGAATTTCCCAGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7IBT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment